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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
08/01/1998 |
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Data da última atualização: |
13/07/2018 |
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Autoria: |
GEETHA, K. B.; LENDING, C. R.; LOPES, M. A.; WALLACE, J. C.; LARKINS, B. A. |
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Título: |
Opaque-2 modifiers increase y-zein synthesis and alter its spatial distribution in maize endosperm. |
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Ano de publicação: |
1991 |
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Fonte/Imprenta: |
Plant Cell, Rockville, v. 3, n. 11, p. 1207-1219, Nov. 1991. |
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Idioma: |
Inglês |
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Conteúdo: |
Through the action of opaque-2 modifier genes, the soft, floury of opaque-2 mutants is converted to a vitreous phenotype. This change in endosperm texture is associated with a twofold to threefold increase in y-zein content. To investigate the effect of opaque-2 modifiers on the expression of y-zein genes, we analyzed the synthesis and distribution of y-zein protein and the level of y-zein mRNAs in developing endosperms of the inbreds W644 and W644o2, a modified opaque-2 mutant Poll 34 QP,, and their reciprocal F1 hybrids. We also characterized the number and organization of y-zein genes in these and related maize genotypes. Our studies show that opaque-2 modifiers are semidominant genes, resulting in a twofold to threefold increase in y-zein gene expression in both opaque-2 and normal genetic backgrounds. The increase in gene expression appears to e a conseguence of copies in modified as well as nonmodified genetic backgrounds. Ultrastructural studies showed that y-zein occurs in high concentration in the first few subaleurone cells of nonmodified endosperm, but high concentrations of y-zein occur in the subaleurone and central endosperm cells of modified opaque-2 mutants. The increased concentration and distribution of y-zein in modified endosperm are highly correlated with the activity of opaque-2 modifier genes. |
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Palavras-Chave: |
Maize; Molecular; Opaco-2; Opaque 2; Protein; QPM. |
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Thesagro: |
Milho; Qualidade; Zea Mays. |
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Thesaurus Nal: |
molecular biology. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 02113naa a2200289 a 4500 001 1478285 005 2018-07-13 008 1991 bl uuuu u00u1 u #d 100 1 $aGEETHA, K. B. 245 $aOpaque-2 modifiers increase y-zein synthesis and alter its spatial distribution in maize endosperm.$h[electronic resource] 260 $c1991 520 $aThrough the action of opaque-2 modifier genes, the soft, floury of opaque-2 mutants is converted to a vitreous phenotype. This change in endosperm texture is associated with a twofold to threefold increase in y-zein content. To investigate the effect of opaque-2 modifiers on the expression of y-zein genes, we analyzed the synthesis and distribution of y-zein protein and the level of y-zein mRNAs in developing endosperms of the inbreds W644 and W644o2, a modified opaque-2 mutant Poll 34 QP,, and their reciprocal F1 hybrids. We also characterized the number and organization of y-zein genes in these and related maize genotypes. Our studies show that opaque-2 modifiers are semidominant genes, resulting in a twofold to threefold increase in y-zein gene expression in both opaque-2 and normal genetic backgrounds. The increase in gene expression appears to e a conseguence of copies in modified as well as nonmodified genetic backgrounds. Ultrastructural studies showed that y-zein occurs in high concentration in the first few subaleurone cells of nonmodified endosperm, but high concentrations of y-zein occur in the subaleurone and central endosperm cells of modified opaque-2 mutants. The increased concentration and distribution of y-zein in modified endosperm are highly correlated with the activity of opaque-2 modifier genes. 650 $amolecular biology 650 $aMilho 650 $aQualidade 650 $aZea Mays 653 $aMaize 653 $aMolecular 653 $aOpaco-2 653 $aOpaque 2 653 $aProtein 653 $aQPM 700 1 $aLENDING, C. R. 700 1 $aLOPES, M. A. 700 1 $aWALLACE, J. C. 700 1 $aLARKINS, B. A. 773 $tPlant Cell, Rockville$gv. 3, n. 11, p. 1207-1219, Nov. 1991.
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| Registros recuperados : 7 | |
| 1. |  | TAMBASCO-STUDART, M.; TAMBASCO, D. D.; ALENCAR, M. M. de; COUTINHO, L. L.; SURIANO, M.; VASCONCELLOS, L. P. M. K.; REGITANO, L. C. de A. Caracterização de animais da raça Simental com base em 10 marcadores moleculares. In: CONGRESSO NACIONAL DE GENÉTICA, 47., 2001. Águas de Lindóia, SP. Anais... Águas de Lindóia: SBG, 2001.| Tipo: Resumo em Anais de Congresso |
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| 2. |  | REGITANO, L. C. de A.; JORGE, E. C.; PACKER, I. U.; BARBOSA, P. F.; VASCONCELLOS, L. P. M. K.; TAMBASCO-STUDART, M.; COUTINHO, L. L. A study of genetic variability in Canchim (5/8 Charolais, 3/8 Zebu) based on 8 molecular markers. In: GLOBAL CONFERENCE ON CONSERVATION OF DOMESTIC ANIMAL GENETIC RESOURCES, 15., 2000, Brasília, DF. Conservation and biotecnology: a balaced appoach for the new millennium - proceedings. Brasília: Embrapa Genetic Resourches Genetic and Biotecnology, 2000.| Tipo: Artigo em Anais de Congresso |
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| 3. |  | TAMBASCO, D. D.; ALENCAR, M. M. de; COUTINHO, L. L.; PACKER, I. U.; TAMBASCO-STUDART, M.; REGITANO, L. C. de A. A survey of Nellore breed based on nine molecular markers. In: GLOBAL CONFERENCE ON CONSERVATION OF DOMESTIC ANIMAL GENETIC RESOURCES, 15., 2000, Brasilia, DF. Conservation and biotecnology: A balaced appoach for the new millennium - proceedings. Brasilia: Embrapa Genetic Resourches Genetic and Biotecnology, 2000. p. Resumo| Tipo: Artigo em Anais de Congresso |
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| 4. |  | TAMBASCO, D. D.; PAZ, C. C. P.; TAMBASCO-STUDART, M.; PEREIRA, A. P.; ALENCAR, M. M. de; FREITAS, A. R. de; COUTINHO, L. L.; PACKER, I. U.; REGITANO, L. C. de A. Candidate genes for growth traits in beef cattle crosses Bos taurus X Bos indicus. In: WORLD CONGRESS APPLIED TO LIVESCTOCK PRODUCTION, 7., 2002, Montpellier, France. Papers... Montpellier: INRA, 2002. 4 p.| Tipo: Artigo em Anais de Congresso |
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| 5. |  | TAMBASCO, D. D.; PAZ, C. C. P.; TAMBASCO-STUDART, M.; PEREIRA, A. P.; ALENCAR, M. M. de; FREITAS, A. R. de; COUTINHO, L. L.; PACKER, I. U.; REGITANO, L. C. de A. Candidate genes for growth traits in beef cattle crosses Bos taurus X Bos indicus. Journal of Animal Breeding and Genetics, v.120, n.1, p.51-56, 2003.| Tipo: Artigo em Periódico Indexado |
| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 6. |  | TAMBASCO, D. D.; PAZ, C. C. P.; TAMBASCO-STUDART, M.; PEREIRA, A. P.; ALENCAR, M. M. de; FREITAS, A. R. de; COUTINHO, L. L.; PACKER, I. U.; REGITANO, L. C. de A. Candidate genes for growth traits in beff cattle crosses Bos taurus X Bos indicus. Journal of Animal Breeding and Genetics, v. 120, n. 1, p. 51-56, Fev. 2003.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
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| 7. |  | REGITANO, L. C. de A.; VASCONCELLOS, L. P. M. K.; JACINTO, E.; TAMBASCO-STUDART, M.; TAMBASCO, D. D.; EUCLIDES FILHO, K.; BARBOSA, P. F.; PACKER, I. U.; COUTINHO, L. L. Genetic distances among Aberdeen Angus, Canchim, Caracu, Nellore and Simmental beef cattle breeds. In: GLOBAL CONFERENCE ON CONSERVATION OF DOMESTIC ANIMAL GENETIC RESOURCES, 15., 2000, Brasilia, DF. Conservation and biotecnology: A balaced appoach for the new millennium - proceedings. Brasilia: Embrapa Genetic Resourches Genetic and Biotecnology, 2000.| Tipo: Artigo em Anais de Congresso |
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| Registros recuperados : 7 | |
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