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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
08/01/1998 |
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Data da última atualização: |
13/07/2018 |
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Autoria: |
GEETHA, K. B.; LENDING, C. R.; LOPES, M. A.; WALLACE, J. C.; LARKINS, B. A. |
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Título: |
Opaque-2 modifiers increase y-zein synthesis and alter its spatial distribution in maize endosperm. |
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Ano de publicação: |
1991 |
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Fonte/Imprenta: |
Plant Cell, Rockville, v. 3, n. 11, p. 1207-1219, Nov. 1991. |
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Idioma: |
Inglês |
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Conteúdo: |
Through the action of opaque-2 modifier genes, the soft, floury of opaque-2 mutants is converted to a vitreous phenotype. This change in endosperm texture is associated with a twofold to threefold increase in y-zein content. To investigate the effect of opaque-2 modifiers on the expression of y-zein genes, we analyzed the synthesis and distribution of y-zein protein and the level of y-zein mRNAs in developing endosperms of the inbreds W644 and W644o2, a modified opaque-2 mutant Poll 34 QP,, and their reciprocal F1 hybrids. We also characterized the number and organization of y-zein genes in these and related maize genotypes. Our studies show that opaque-2 modifiers are semidominant genes, resulting in a twofold to threefold increase in y-zein gene expression in both opaque-2 and normal genetic backgrounds. The increase in gene expression appears to e a conseguence of copies in modified as well as nonmodified genetic backgrounds. Ultrastructural studies showed that y-zein occurs in high concentration in the first few subaleurone cells of nonmodified endosperm, but high concentrations of y-zein occur in the subaleurone and central endosperm cells of modified opaque-2 mutants. The increased concentration and distribution of y-zein in modified endosperm are highly correlated with the activity of opaque-2 modifier genes. |
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Palavras-Chave: |
Maize; Molecular; Opaco-2; Opaque 2; Protein; QPM. |
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Thesagro: |
Milho; Qualidade; Zea Mays. |
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Thesaurus Nal: |
molecular biology. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 02113naa a2200289 a 4500
001 1478285
005 2018-07-13
008 1991 bl uuuu u00u1 u #d
100 1 $aGEETHA, K. B.
245 $aOpaque-2 modifiers increase y-zein synthesis and alter its spatial distribution in maize endosperm.$h[electronic resource]
260 $c1991
520 $aThrough the action of opaque-2 modifier genes, the soft, floury of opaque-2 mutants is converted to a vitreous phenotype. This change in endosperm texture is associated with a twofold to threefold increase in y-zein content. To investigate the effect of opaque-2 modifiers on the expression of y-zein genes, we analyzed the synthesis and distribution of y-zein protein and the level of y-zein mRNAs in developing endosperms of the inbreds W644 and W644o2, a modified opaque-2 mutant Poll 34 QP,, and their reciprocal F1 hybrids. We also characterized the number and organization of y-zein genes in these and related maize genotypes. Our studies show that opaque-2 modifiers are semidominant genes, resulting in a twofold to threefold increase in y-zein gene expression in both opaque-2 and normal genetic backgrounds. The increase in gene expression appears to e a conseguence of copies in modified as well as nonmodified genetic backgrounds. Ultrastructural studies showed that y-zein occurs in high concentration in the first few subaleurone cells of nonmodified endosperm, but high concentrations of y-zein occur in the subaleurone and central endosperm cells of modified opaque-2 mutants. The increased concentration and distribution of y-zein in modified endosperm are highly correlated with the activity of opaque-2 modifier genes.
650 $amolecular biology
650 $aMilho
650 $aQualidade
650 $aZea Mays
653 $aMaize
653 $aMolecular
653 $aOpaco-2
653 $aOpaque 2
653 $aProtein
653 $aQPM
700 1 $aLENDING, C. R.
700 1 $aLOPES, M. A.
700 1 $aWALLACE, J. C.
700 1 $aLARKINS, B. A.
773 $tPlant Cell, Rockville$gv. 3, n. 11, p. 1207-1219, Nov. 1991.
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| 1. |  | MEDEIROS, R. L. S.; SOUZA, V. C. de; BARBOSA NETO, M. A.; ARAÚJO, L. de; BARBOSA, A. de S.; MEDEIROS, R. L. S. de. Estrutura da regeneração natural de Anadenanthera colubrina em fragmento de brejo de altitude em Bananeiras, PB. Pesquisa Florestal Brasileira, Colombo, v. 36, n. 86, p. 95-101, abr./jun. 2016.| Biblioteca(s): Embrapa Florestas. |
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