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 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
06/01/1998 |
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Data da última atualização: |
30/04/2025 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
LOPES, M. A.; COLEMAN, C. E.; KADRZYCKI, R.; LENDING, C. R.; LARKINS, B. A. |
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Afiliação: |
EMBRAPA/CNPMS. |
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Título: |
Synthesis of an unusual α-zein protein is correlated with the phenotypic effects of the floury2 mutation in maize. |
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Ano de publicação: |
1994 |
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Fonte/Imprenta: |
Molecular and General Genetics, New York, v. 245, p. 537-547, 1994. |
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Idioma: |
Inglês |
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Conteúdo: |
The soft, starchy endosperm of the maize (Zea mays L.) floury2 mutant is associated with a reduction in zein mRNA and protein synthesis, unique protein body morphology, and enhanced levels of a 70 kDa protein, that has been shown to be the maize homolog of a chaperonin found in the endoplasmic reticulum. We found as unusual x-zein protein of 24 kDa to be consistently associated with the zein fraction from floury2 mutants. Three additional x-zein proteins with molecular weights ranging from ca. 25 to 27 kDa are detected in the storage protein fraction of a high percentage of floury2 kernels and a low percentage of normal kernels in a genetically segregating population. The four proteins can be distinguished from one another by immunostaining on Western blots. Synthesis of the 24 kDa protein is regulated by Opaque2, since the 24 kDa protein is lacking in the storage protein fraction of opaque2/floury2 double mutants. The synthesis of an abnormal x-zein protein in floury2 could explain many features of the mutants, such as the abnormal protein body morphology, induction of the 70 kDa chaperonin, and hypostasis to opaque2 (o2). Although we cannot prove that the accumulation of this protein is responsible for the floury2 phenotype, we were able to detect a restriction fragment length polymorphism (RFLP) linked to the floury2 locus with a 22 kDa x-zein probe. We hypothesize that the unique characteristics of the floury2 mutant could be a response to the accumulation of a defective x-zein protein which impairs secretory protein synthesis. MenosThe soft, starchy endosperm of the maize (Zea mays L.) floury2 mutant is associated with a reduction in zein mRNA and protein synthesis, unique protein body morphology, and enhanced levels of a 70 kDa protein, that has been shown to be the maize homolog of a chaperonin found in the endoplasmic reticulum. We found as unusual x-zein protein of 24 kDa to be consistently associated with the zein fraction from floury2 mutants. Three additional x-zein proteins with molecular weights ranging from ca. 25 to 27 kDa are detected in the storage protein fraction of a high percentage of floury2 kernels and a low percentage of normal kernels in a genetically segregating population. The four proteins can be distinguished from one another by immunostaining on Western blots. Synthesis of the 24 kDa protein is regulated by Opaque2, since the 24 kDa protein is lacking in the storage protein fraction of opaque2/floury2 double mutants. The synthesis of an abnormal x-zein protein in floury2 could explain many features of the mutants, such as the abnormal protein body morphology, induction of the 70 kDa chaperonin, and hypostasis to opaque2 (o2). Although we cannot prove that the accumulation of this protein is responsible for the floury2 phenotype, we were able to detect a restriction fragment length polymorphism (RFLP) linked to the floury2 locus with a 22 kDa x-zein probe. We hypothesize that the unique characteristics of the floury2 mutant could be a response to the accumulation of a defective... Mostrar Tudo |
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Thesagro: |
Melhoramento genético vegetal; Milho. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02177naa a2200193 a 4500
001 1478272
005 2025-04-30
008 1994 bl uuuu u00u1 u #d
100 1 $aLOPES, M. A.
245 $aSynthesis of an unusual α-zein protein is correlated with the phenotypic effects of the floury2 mutation in maize.$h[electronic resource]
260 $c1994
520 $aThe soft, starchy endosperm of the maize (Zea mays L.) floury2 mutant is associated with a reduction in zein mRNA and protein synthesis, unique protein body morphology, and enhanced levels of a 70 kDa protein, that has been shown to be the maize homolog of a chaperonin found in the endoplasmic reticulum. We found as unusual x-zein protein of 24 kDa to be consistently associated with the zein fraction from floury2 mutants. Three additional x-zein proteins with molecular weights ranging from ca. 25 to 27 kDa are detected in the storage protein fraction of a high percentage of floury2 kernels and a low percentage of normal kernels in a genetically segregating population. The four proteins can be distinguished from one another by immunostaining on Western blots. Synthesis of the 24 kDa protein is regulated by Opaque2, since the 24 kDa protein is lacking in the storage protein fraction of opaque2/floury2 double mutants. The synthesis of an abnormal x-zein protein in floury2 could explain many features of the mutants, such as the abnormal protein body morphology, induction of the 70 kDa chaperonin, and hypostasis to opaque2 (o2). Although we cannot prove that the accumulation of this protein is responsible for the floury2 phenotype, we were able to detect a restriction fragment length polymorphism (RFLP) linked to the floury2 locus with a 22 kDa x-zein probe. We hypothesize that the unique characteristics of the floury2 mutant could be a response to the accumulation of a defective x-zein protein which impairs secretory protein synthesis.
650 $aMelhoramento genético vegetal
650 $aMilho
700 1 $aCOLEMAN, C. E.
700 1 $aKADRZYCKI, R.
700 1 $aLENDING, C. R.
700 1 $aLARKINS, B. A.
773 $tMolecular and General Genetics, New York$gv. 245, p. 537-547, 1994.
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Embrapa Milho e Sorgo (CNPMS) |
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| 12. |  | ALMEIDA, L. A.; KIIHL, R. A. S.; YORINORI, J. T.; HARADA, A.; FONSECA, N.; TERASAWA, F.; BOYE, R.; AGUIAR, C. Ensaio intermediario de avaliacao de linhagens. In: EMBRAPA. Centro Nacional de Pesquisa de Soja (Londrina, PR). Resultados de pesquisa de soja 1984/85. Londrina, 1985. p.243-247 (EMBRAPA-CNPSo. Documentos, 15).| Biblioteca(s): Embrapa Soja. |
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| 13. | | MENOSSO, O. G.; HARADA, A.; SIMONETTI, F.; TERASAWA, F.; GABE, H. L.; CARRARO, I. M.; GRODZKI, L. Avaliacao final de linhagem de soja para o Estado do Parana. In: REUNIAO DE PESQUISA DE SOJA DA REGIAO SUL, 9., 1981, Passo Fundo. Ata... Passo Fundo: EMBRAPA-CNPT, 1981. p.165.| Biblioteca(s): Embrapa Soja. |
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| 14. | | SUZUKI, S.; HARADA, A.; SILVEIRA, J. M.; MENOSSO, O. G.; TERASAWA, F.; BOYE, R.; HIGASHI, W. H. Avaliacao final de linhagens em semeadura de novembro. In: OCEPAR. Programa de Pesquisa (Cascavel, PR). Resultados de pesquisa verao 1987/88. Cascavel, 1989. p.62-70. (OCEPAR. Resultados de Pesquisa, 3).| Biblioteca(s): Embrapa Soja. |
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| 17. | | SILVA FILHO, P. M. da; KIIHL, R. A. S.; YORINORI, J. T.; FONSECA JUNIOR, N.; TERASAWA, F.; BOYE, R.; AGUIAR, C. Ensaio intermediario de avaliacao de linhagens. In: EMBRAPA. Centro Nacional de Pesquisa de Soja (Londrina, PR). Resultados de pesquisa de soja 1985/86. Londrina, 1987. p.261-265. (EMBRAPA-CNPSo. Documentos, 20).| Biblioteca(s): Embrapa Soja. |
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| 18. | | HARADA, A.; MENOSSO, O. G.; TERASAWA, F.; AGUIAR, C. G. de; KIIHL, R. A. S.; SILVEIRA, J. M.; BENI, O. A.; ROCHA, A.; BEGO, A. Ensaios finais em semeadura convencional. In: OCEPAR. Programa de Pesquisa (Cascavel, PR). Resultados de pesquisa na safra de verao 1986/87. Cascavel, 1988. p.46-57. (OCEPAR. Resultados de Pesquisa, 1).| Biblioteca(s): Embrapa Soja. |
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| 19. | | HARADA, A.; KIIHL, R. A. S.; SILVEIRA, J. M.; AGUIAR, C. G. de; TERASAWA, F.; IGASHI, W. E.; BENI, O. A.; ROCHA, A. Ensaios intermediarios de semeadura em epoca convencional. In: OCEPAR. Programa de Pesquisa (Cascavel, PR). Resultados de pesquisa na safra de verao 1986/87. Cascavel, 1988. p.36-43. (OCEPAR. Resultados de Pesquisa, 1).| Biblioteca(s): Embrapa Soja. |
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| 20. | | FONSECA JUNIOR, N. S.; MENOSSO, O. G.; HARADA, A.; TERASAWA, F.; AGUIAR, C. G.; BENI, O. A.; ROCHA, A.; BEGO, A. Introducao e competicao de cultivares e linhagens de soja: ensaios finais (semeadura em epoca convencional). In: OCEPAR. Programa de Pesquisa (Cascavel, PR). Resultados de pesquisa na safra de verao: 1985/86. Cascavel, 1986. p.24-34.| Biblioteca(s): Embrapa Soja. |
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| Registros recuperados : 34 | |
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