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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
20/02/2009 |
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Data da última atualização: |
25/07/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BARCELLOS, F. G.; BATISTA, J. S. da S.; MENNA, P.; HUNGRIA, M. |
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Afiliação: |
FERNANDO GOMES BARCELLOS, UEL; JESIANE STEFÂNIA DA SILVA BATISTA, UEL; PÂMELA MENNA, UEL; MARIANGELA HUNGRIA DA CUNHA, CNPSO. |
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Título: |
Genetic differences between Bradyrhizobium japonicum variant strains contrasting in N2-fixation efficiency revealed by representational difference analysis. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
Archives of Microbiology, v. 191, n. 2, p. 113-122, Feb. 2009. |
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ISSN: |
0302-8933 |
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DOI: |
10.1007/s00203-008-0432-0 |
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Idioma: |
Inglês |
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Conteúdo: |
Two variant strains of Bradyrhizobium japonicum, derived from SEMIA 566, adapted to the stressful environmetal conditions of the Brazilian Cerrados and characterized by contrasting capacities for N2 fixation, were compared by representational difference analysis (RDA). Twenty-four gene sequences that are unique to the highly effective strain S 370 were identified, eight showing high similarity to known genes, nine encoding putative proteins and seven representing conserved hypothetical or hypothetical proteins: they were classified in eight functional categories. Among those genes, some were highlighted for their known or potential funcitions in plant-microbe interactions. The nodulation outer protein P (nopP), related to the type-III secretion system (TTSS) and a major determinant of nodulation of some tropical legumes, was detected in the genome of strain S 370. Three coding sequences (CDS) identified by RDA were expressed in proteomics experiments with B. japonicum strain USDA 110 (ChvE and NopP). The use of the sequences identified by RDA in the highly effective strain S 370 might represent an important tool to speed up strain selection programs, accelerating prescreening procedures. Additionally, the conserved hypothetical and hypothetical proteins idenfied in strain S 370 might encode important but still unknown protein related to the symbiosis that deserve further study. |
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Thesagro: |
Bactéria; Bradyrhizobium Japonicum; Fixação de Nitrogênio; Nodulação; Soja. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02166naa a2200241 a 4500
001 1471311
005 2017-07-25
008 2009 bl uuuu u00u1 u #d
022 $a0302-8933
024 7 $a10.1007/s00203-008-0432-0$2DOI
100 1 $aBARCELLOS, F. G.
245 $aGenetic differences between Bradyrhizobium japonicum variant strains contrasting in N2-fixation efficiency revealed by representational difference analysis.
260 $c2009
520 $aTwo variant strains of Bradyrhizobium japonicum, derived from SEMIA 566, adapted to the stressful environmetal conditions of the Brazilian Cerrados and characterized by contrasting capacities for N2 fixation, were compared by representational difference analysis (RDA). Twenty-four gene sequences that are unique to the highly effective strain S 370 were identified, eight showing high similarity to known genes, nine encoding putative proteins and seven representing conserved hypothetical or hypothetical proteins: they were classified in eight functional categories. Among those genes, some were highlighted for their known or potential funcitions in plant-microbe interactions. The nodulation outer protein P (nopP), related to the type-III secretion system (TTSS) and a major determinant of nodulation of some tropical legumes, was detected in the genome of strain S 370. Three coding sequences (CDS) identified by RDA were expressed in proteomics experiments with B. japonicum strain USDA 110 (ChvE and NopP). The use of the sequences identified by RDA in the highly effective strain S 370 might represent an important tool to speed up strain selection programs, accelerating prescreening procedures. Additionally, the conserved hypothetical and hypothetical proteins idenfied in strain S 370 might encode important but still unknown protein related to the symbiosis that deserve further study.
650 $aBactéria
650 $aBradyrhizobium Japonicum
650 $aFixação de Nitrogênio
650 $aNodulação
650 $aSoja
700 1 $aBATISTA, J. S. da S.
700 1 $aMENNA, P.
700 1 $aHUNGRIA, M.
773 $tArchives of Microbiology$gv. 191, n. 2, p. 113-122, Feb. 2009.
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