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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
16/02/2005 |
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Data da última atualização: |
11/05/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FUNES-HUACCA, M.; REGITANO, L. C. de A.; MURLLER, O.; CARILLHO, E. |
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Afiliação: |
MARIBEL FUNES-HUACCA, USP; LUCIANA CORREIA DE ALMEIDA REGITANO, CPPSE; ODILO MUELLER, Agilent Technologies, Waldbronn, Germany; EMANUEL CARRILHO, USP. |
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Título: |
Semiquantitative determination of Alicyclobacillus acidoterrestris in orange juice by reverse-transcriptase polymerase chain reaction and capillary electrophoresis: laser induced fluorescence using microchip technology. |
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Ano de publicação: |
2004 |
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Fonte/Imprenta: |
Electrophoresis, v.25, n.21-22, p.3860-3864, 2004. |
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DOI: |
https://doi.org/10.1002/elps.200406105 |
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Idioma: |
Inglês |
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Conteúdo: |
The semiquantitative detection ofAlicyclobacillus acidoterrrestrisin orange juice byreverse-transcriptase polymerase chain reaction (RT-PCR) with a linear dynamic rangeof 26105– 2 colony forming units (CFU)/mL in terms of cell count is described.Separation, detection, and quantification of the RT-PCR products were accomplishedusing the Agilent 2100 bioanalyzer in conjunction with the DNA 1000 LabChip kit. After0 and 12 h of enrichment, it was possible to generate a linear standard curve betweenthe amount of cells and amplicon concentration of RT-PCR and PCR products. Usingthis method, cell diminution was verified in samples of orange juice treated with a nat-ural inhibitor (Sapindus saponaria), determining the persistence of viable cells. Semi-quantitative RT-PCR using the Agilent 2100 bioanalyzer is a potentially useful approachfor rapidin vitrodetermination ofA. acidoterrestrisand monitoring of inhibitor sus-ceptibility for the orange juice-producing industry. |
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Palavras-Chave: |
Miniaturization; Reversetranscriptase. |
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Thesaurus Nal: |
Alicyclobacillus; Alicyclobacillus acidoterrestris; Microchip technology; Orange juice; Reverse transcriptase polymerase chain reaction. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 01933naa a2200253 a 4500
001 1046990
005 2022-05-11
008 2004 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1002/elps.200406105$2DOI
100 1 $aFUNES-HUACCA, M.
245 $aSemiquantitative determination of Alicyclobacillus acidoterrestris in orange juice by reverse-transcriptase polymerase chain reaction and capillary electrophoresis$blaser induced fluorescence using microchip technology.$h[electronic resource]
260 $c2004
520 $aThe semiquantitative detection ofAlicyclobacillus acidoterrrestrisin orange juice byreverse-transcriptase polymerase chain reaction (RT-PCR) with a linear dynamic rangeof 26105– 2 colony forming units (CFU)/mL in terms of cell count is described.Separation, detection, and quantification of the RT-PCR products were accomplishedusing the Agilent 2100 bioanalyzer in conjunction with the DNA 1000 LabChip kit. After0 and 12 h of enrichment, it was possible to generate a linear standard curve betweenthe amount of cells and amplicon concentration of RT-PCR and PCR products. Usingthis method, cell diminution was verified in samples of orange juice treated with a nat-ural inhibitor (Sapindus saponaria), determining the persistence of viable cells. Semi-quantitative RT-PCR using the Agilent 2100 bioanalyzer is a potentially useful approachfor rapidin vitrodetermination ofA. acidoterrestrisand monitoring of inhibitor sus-ceptibility for the orange juice-producing industry.
650 $aAlicyclobacillus
650 $aAlicyclobacillus acidoterrestris
650 $aMicrochip technology
650 $aOrange juice
650 $aReverse transcriptase polymerase chain reaction
653 $aMiniaturization
653 $aReversetranscriptase
700 1 $aREGITANO, L. C. de A.
700 1 $aMURLLER, O.
700 1 $aCARILLHO, E.
773 $tElectrophoresis$gv.25, n.21-22, p.3860-3864, 2004.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
