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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
01/07/2004 |
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Data da última atualização: |
04/04/2025 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
PINEDO, F. J. R.; MOSCARDI, F.; LUQUE, T.; OLSZEWSKI, J. A.; RIBEIRO, B. M. |
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Afiliação: |
UNIVERSIDADE DE BRASÍLIA; FLAVIO MOSCARDI, CNPSO; IMPERIAL COLLEGE OF SCIENCE, TECHNOLOGY AND MEDICINE; IMPERIAL COLLEGE OF SCIENCE, TECHNOLOGY AND MEDICINE; UNIVERSIDADE DE BRASÍLIA. |
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Título: |
Inactivation of the ecdysteroid UDP-glucosyltransferase (egt) gene of Anticarsia gemmatalis nucleopolyhedrovirus (AgMNPV) improves its virulence towards its insect host. |
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Ano de publicação: |
2003 |
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Fonte/Imprenta: |
Biological Control, v. 27, n. 3, p. 336-344, July 2003. |
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DOI: |
10.1016/S1049-9644(03)00026-4 |
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Idioma: |
Inglês |
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Conteúdo: |
Some baculovirus have been genetically modified for the inactivation of their ecdysteroid glucosyltransferase (egt) gene, and these viruses were shown to kill infected larvae more rapidly when compared to wild-type virus infections. We have previously identified, cloned, and sequenced the egt gene of Anticarsia gemmatalis nucleopolyhedrovirus (AgMNPV). Here we present data regarding the construction of an egt minus (egt?x2212;) AgMNPV and its virulence towards its insect host. We have inserted an hsp70-lacZ (3.7 kb) gene cassette into the egt gene open reading frame (ORF) and purified a recombinant AgMNPV (vAgEGT?x394;-lacZ). Bioassays with third-instar A. gemmatalis larvae showed that viral occlusion body (OB) production were consistently lower from infections with vAgEGT?x394;-lacZ compared to the wild-type virus. A mean of 20.4Ã?108 OBs/g/larva and 40.7Ã?108 OBs/g/larva was produced from vAgEGT?x394;-lacZ and AgMNPV infections, respectively. The mean lethal concentration which killed 50% of insects in a treatment group (LC50) for the 10th day after virus treatment (DAT) was 3.9-fold higher for the wild-type virus compared to vAgEGT?x394;-lacZ. The recombinant virus killed A. gemmatalis larvae significantly faster (ca. 1?x2013;2.8 days), than the wild-type AgMNPV. Therefore, the vAgEGT?x394;-lacZ was more efficacious for the control of A. gemmatalis larvae (in bioassays) compared to wild-type AgMNPV. |
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Palavras-Chave: |
Ecdysteroid glucosyltransferase; Velvetbean caterpillar. |
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Thesagro: |
Anticarsia Gemmatalis; Baculovirus; Controle Biológico; Engenharia Genética. |
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Thesaurus Nal: |
Baculoviridae; Biological control; Genetic engineering. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02398naa a2200289 a 4500
001 1467192
005 2025-04-04
008 2003 bl uuuu u00u1 u #d
024 7 $a10.1016/S1049-9644(03)00026-4$2DOI
100 1 $aPINEDO, F. J. R.
245 $aInactivation of the ecdysteroid UDP-glucosyltransferase (egt) gene of Anticarsia gemmatalis nucleopolyhedrovirus (AgMNPV) improves its virulence towards its insect host.$h[electronic resource]
260 $c2003
520 $aSome baculovirus have been genetically modified for the inactivation of their ecdysteroid glucosyltransferase (egt) gene, and these viruses were shown to kill infected larvae more rapidly when compared to wild-type virus infections. We have previously identified, cloned, and sequenced the egt gene of Anticarsia gemmatalis nucleopolyhedrovirus (AgMNPV). Here we present data regarding the construction of an egt minus (egt?x2212;) AgMNPV and its virulence towards its insect host. We have inserted an hsp70-lacZ (3.7 kb) gene cassette into the egt gene open reading frame (ORF) and purified a recombinant AgMNPV (vAgEGT?x394;-lacZ). Bioassays with third-instar A. gemmatalis larvae showed that viral occlusion body (OB) production were consistently lower from infections with vAgEGT?x394;-lacZ compared to the wild-type virus. A mean of 20.4Ã?108 OBs/g/larva and 40.7Ã?108 OBs/g/larva was produced from vAgEGT?x394;-lacZ and AgMNPV infections, respectively. The mean lethal concentration which killed 50% of insects in a treatment group (LC50) for the 10th day after virus treatment (DAT) was 3.9-fold higher for the wild-type virus compared to vAgEGT?x394;-lacZ. The recombinant virus killed A. gemmatalis larvae significantly faster (ca. 1?x2013;2.8 days), than the wild-type AgMNPV. Therefore, the vAgEGT?x394;-lacZ was more efficacious for the control of A. gemmatalis larvae (in bioassays) compared to wild-type AgMNPV.
650 $aBaculoviridae
650 $aBiological control
650 $aGenetic engineering
650 $aAnticarsia Gemmatalis
650 $aBaculovirus
650 $aControle Biológico
650 $aEngenharia Genética
653 $aEcdysteroid glucosyltransferase
653 $aVelvetbean caterpillar
700 1 $aMOSCARDI, F.
700 1 $aLUQUE, T.
700 1 $aOLSZEWSKI, J. A.
700 1 $aRIBEIRO, B. M.
773 $tBiological Control$gv. 27, n. 3, p. 336-344, July 2003.
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