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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
16/08/2000 |
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Data da última atualização: |
08/09/2023 |
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Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
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Autoria: |
HUNGRIA, M.; ANDRADE, D. S.; CHUEIRE, L. M. de O.; MEGIAS, M. |
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Afiliação: |
MARIANGELA HUNGRIA DA CUNHA, CNPSO; D. S. ANDRADE, IAPAR; L. M. DE O. CHUEIRE; M. MEGÍAS, UNIV. DE SEVILLA. |
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Título: |
Characterization of new efficient and competitive strains for the bean (Phaseolus vulgaris L.) crop in Brazil. |
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Ano de publicação: |
1999 |
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Fonte/Imprenta: |
In: MARTINEZ, E.; HERNANDEZ, G. (ed.). Highlights of nitrogen fixation research. New York: Kluwer / Plemum, 1999. |
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Páginas: |
p. 251-254. |
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Idioma: |
Inglês |
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Conteúdo: |
A lack of response to bean inoculation in field experiments performed in Brazil has been frequently, but a long term program of selection of strains genetically stable and adpted to environmentally stressing conditions is missing. In the State of Parana, three strains were identified, PRF 35, PRF 81, which have shown high rates of N2 fixation, competitiveness, and tolerance to high temperatures under greenhouse conditions. The good performance, especially of PRF 81, was confirmed in several field trials and this strain has then been recommended, since 1996, for the use in Brazilian commercial inoculants. The three strains from Parana were better characterized trying to define parameters which could help the selection of bean rhizobia. The strains have shown the same Nod factor profile as Rhizobium tropici type IIA CFN 299 and type IIB CIAT 899. The colony morphology, ability to grow in vitro at low pH and high temperatures, and PCR product with Y1-Y2 primers were similar to CIAT 899, but the strains were not able to grow in LB or PY minus Ca media, similary to CFN 299. The results reported here simulate the search of new R. tropici strains for tropical and subtropical bean cropping areas. |
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Palavras-Chave: |
Bean; Brasil; Parana. |
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Thesagro: |
Feijão; Fixação de Nitrogênio. |
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Thesaurus Nal: |
Brazil; nitrogen fixation. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 01987naa a2200253 a 4500
001 1462109
005 2023-09-08
008 1999 bl uuuu u00u1 u #d
100 1 $aHUNGRIA, M.
245 $aCharacterization of new efficient and competitive strains for the bean (Phaseolus vulgaris L.) crop in Brazil.$h[electronic resource]
260 $c1999
300 $ap. 251-254.
520 $aA lack of response to bean inoculation in field experiments performed in Brazil has been frequently, but a long term program of selection of strains genetically stable and adpted to environmentally stressing conditions is missing. In the State of Parana, three strains were identified, PRF 35, PRF 81, which have shown high rates of N2 fixation, competitiveness, and tolerance to high temperatures under greenhouse conditions. The good performance, especially of PRF 81, was confirmed in several field trials and this strain has then been recommended, since 1996, for the use in Brazilian commercial inoculants. The three strains from Parana were better characterized trying to define parameters which could help the selection of bean rhizobia. The strains have shown the same Nod factor profile as Rhizobium tropici type IIA CFN 299 and type IIB CIAT 899. The colony morphology, ability to grow in vitro at low pH and high temperatures, and PCR product with Y1-Y2 primers were similar to CIAT 899, but the strains were not able to grow in LB or PY minus Ca media, similary to CFN 299. The results reported here simulate the search of new R. tropici strains for tropical and subtropical bean cropping areas.
650 $aBrazil
650 $anitrogen fixation
650 $aFeijão
650 $aFixação de Nitrogênio
653 $aBean
653 $aBrasil
653 $aParana
700 1 $aANDRADE, D. S.
700 1 $aCHUEIRE, L. M. de O.
700 1 $aMEGIAS, M.
773 $tIn: MARTINEZ, E.; HERNANDEZ, G. (ed.). Highlights of nitrogen fixation research. New York: Kluwer / Plemum, 1999.
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