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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
01/12/1992 |
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Data da última atualização: |
01/12/1992 |
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Autoria: |
CATSIMPOOLAS, N.; ROGERS, D. A.; MEYER, E. W. |
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Título: |
Immunochemical and disc electrophoresis study of soybean trypsin inhibitor SBTIA-2. |
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Ano de publicação: |
1969 |
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Fonte/Imprenta: |
Cereal Chemistry, v.46, p.136-144, mar. 1969. |
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Descrição Física: |
il. |
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Idioma: |
Inglês |
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Conteúdo: |
The immunochemical properties of the soybean trypsin inhibitor SBTIA-2 were studied by double gel diffusion, immunoelectrophoresis, single immunodiffusion, and complement fixation with the use of an antisoybean water-extract serum 106. As little as 0.03 microgram of the inhibitor can be detected by the micro complement fixation method with 400-fold dilution of antiserum. The inhibitor was found to be immunochemically identical with a corresponding protein in the total soybean water extract, and thus it is not an artifact of isolation. Stoichiometric addition of trypsin greatly reduces the antigenic sites of the inhibitor as examined by complement fixation. The single immunodiffusion technique can be used for quantitative estimation of inhibitor in the range of 0.02 to 1.00 mg. of the compound. By disc electrophoresis, impurities present in the commercial preparation of the inhibitor exhibit distinctly different patterns in presence and absence of persulfate catalyst, and in gels of various polyacrylamide concentrations. Microdensitometer tracings of the stained bands showed that the inhibitor contained the following amounts of apparent impurities in gels polymerized as indicated: 4% gels, ammonium persulfate, none; 7% riboflavin and light, 5%; 7%, ammonium persulfate, 43%; and 11%, riboflavin and light, 22%. The possibility of artifact formation caused by the persulfate catalyst is discussed. |
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Palavras-Chave: |
Disc electrophoresis; Disco; Imuno-difusao radial; Imunoeletroforese; Inibidor de tripsina; Radial immunodiffusion; SBTIA-2; Soybean; Trypsin inhibitor. |
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Thesagro: |
Eletroforese; Soja. |
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Thesaurus Nal: |
immunoelectrophoresis. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02225naa a2200301 a 4500
001 1457479
005 1992-12-01
008 1969 bl --- 0-- u #d
100 1 $aCATSIMPOOLAS, N.
245 $aImmunochemical and disc electrophoresis study of soybean trypsin inhibitor SBTIA-2.
260 $c1969
300 $cil.
520 $aThe immunochemical properties of the soybean trypsin inhibitor SBTIA-2 were studied by double gel diffusion, immunoelectrophoresis, single immunodiffusion, and complement fixation with the use of an antisoybean water-extract serum 106. As little as 0.03 microgram of the inhibitor can be detected by the micro complement fixation method with 400-fold dilution of antiserum. The inhibitor was found to be immunochemically identical with a corresponding protein in the total soybean water extract, and thus it is not an artifact of isolation. Stoichiometric addition of trypsin greatly reduces the antigenic sites of the inhibitor as examined by complement fixation. The single immunodiffusion technique can be used for quantitative estimation of inhibitor in the range of 0.02 to 1.00 mg. of the compound. By disc electrophoresis, impurities present in the commercial preparation of the inhibitor exhibit distinctly different patterns in presence and absence of persulfate catalyst, and in gels of various polyacrylamide concentrations. Microdensitometer tracings of the stained bands showed that the inhibitor contained the following amounts of apparent impurities in gels polymerized as indicated: 4% gels, ammonium persulfate, none; 7% riboflavin and light, 5%; 7%, ammonium persulfate, 43%; and 11%, riboflavin and light, 22%. The possibility of artifact formation caused by the persulfate catalyst is discussed.
650 $aimmunoelectrophoresis
650 $aEletroforese
650 $aSoja
653 $aDisc electrophoresis
653 $aDisco
653 $aImuno-difusao radial
653 $aImunoeletroforese
653 $aInibidor de tripsina
653 $aRadial immunodiffusion
653 $aSBTIA-2
653 $aSoybean
653 $aTrypsin inhibitor
700 1 $aROGERS, D. A.
700 1 $aMEYER, E. W.
773 $tCereal Chemistry$gv.46, p.136-144, mar. 1969.
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Registro original: |
Embrapa Soja (CNPSO) |
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| Registros recuperados : 8 | |
| 1. |  | BERNARDES FILHO, R.; NAIME, J. de M.; VIDAL, R.; MALASPINA, O.; CHAUD NETTO, J. Ativímetro para estudos de comportamento em colonias de Apis mellifera. In: ENCONTRO BRASILEIRO SOBRE BIOLOGIA DE ABELHAS E OUTROS INSETOS SOCIAIS, set. 1992, Ribeirao Preto, SP. Anais... Naturalia, Sao Paulo, n.esp., p.266-267, 1992.| Biblioteca(s): Embrapa Instrumentação. |
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| 2. | | PIRES, C. S. S.; GAZZONI, D. L.; NOCELLI, R. C. F.; MALASPINA, O. Agentes estressores. In: DRUMOND, P. M.; CARVALHO-ZILSE, G. A.; WITTER, S.; ALVES, R. M. de O.; DRUMMOND, M. S. (ed.). Meliponicultura: o produtor pergunta, a Embrapa responde. Brasília, DF: Embrapa, 2024. Cap. 7. p. 75-84| Tipo: Capítulo em Livro Técnico-Científico |
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| 3. | | VIANA-SILVA, F.; PIRES, C.; TOREZANI, K.; BORGES, L.; CHAM, K.; DIAS, C.; TEIXEIRA, I.; TONELLI, C.; BELCHIOR, C.; MARCONDES, C.; NOCELLI, R.; MALASPINA, O.; CIONE, A.; SHIWA, A.; FERRAZ, A. Selection matrix for Brazilian bee species to risk assessment of pesticides. In: INTERNATIONAL SYMPOSIUM OF THE ICP-PR BEE PROTECTION GROUP, 13., 2017, Valencia. Proceedings. Quedlinburg: Julius Kühn-Institut, 2018. (Julius-Kühn-Archiv, 462). p. 56- 61| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 4. | | NOCELLI, R. C. F.; PIRES, C.; TOREZANI, K.; CIONE, A. P.; SHIWA, A.; TONELLI, C.; MARCONDES, C.; BELCHIOR, C.; VIANA-SILVA, F.; GUIMARÃES, G.; TEIXEIRA, I.; CHAM, K.; BORGES, L.; MALASPINA, O. Matrix of selection for Brazilian bee species to risk assessement. In: INTERNATIONAL SYMPOSIUM HAZARDS OF PESTICIDES TO BEES, 13., 2017, Valencia. [Abstracts...]Ontario: International Commission for Plant Pollinator Relationships (ICPPR), 2017.| Tipo: Resumo em Anais de Congresso |
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| 5. | | GUIMARÃES-CESTARO, L.; MARTINS, M. F.; LUÍS CARLOS MARTÍNEZ; MARIA LUISA TELES MARQUES FLORÊNCIO ALVES; KARINA ROSA GUIDUGLI-LAZZARINI; ROBERTA CORNÉLIO FERREIRA NOCELLI; MALASPINA, O.; SERRÃO, J. E.; TEIXEIRA, É. W. Occurrence of virus, microsporidia, and pesticide residues in three species of stingless bees (Apidae: Meliponini) in the field. NATURWISSENSCHAFTEN - The Science of Nature, v. 107, n. 16, 2020. 14 p.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 6. | | GUIMARÃES-CESTARO, L.; SERRÃO, J. E.; MARTINS, M. F.; MARTÍNEZ, L. C.; ALVES, M. L. T. M. F.; LAZZARINI, K. R. G.; NOCELLI, R. C. F.; MALASPINA, O.; EVANS, J. D.; TEIXEIRA, E. W. Viruses, microsporidia and glyphosate residues in Africanized honey bees Apis mellifera, under field conditions. Journal of Apicultural Research, v. 63, n. 2, p. 245-255, 2024.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 3 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 7. | | REBELO, R.; MÁXIMO, D.; CHAM, K.; DIAS, C.; VIANA-SILVA, F.; BORGES, L.; TEIXEIRA, I.; SOUZA, R.; MARCONDES, C.; BELCHIOR, C.; PIRES, C. S. S.; SCORZA JUNIOR, R.; WAICHMAN, A.; OLIVEIRA, R.; MALASPINA, O.; NOCELLI, R.; TORNISIELO, A.; CIONE, A.; SHIWA, A.; MURAKAMI, L.; PATINO, X.; FERRAZ, A.; GUIMARÃES, G.; TONELLI, C. Research needs to improve ecological risk assessment in Brazil. In: SETAC Latin America, 12., BiennalMeeting, 2017, Santos, São Paulo. Abstract Book... Santos, SP: Society of Environmental toxicology and chemistry (SETAC), 2017. resumo 107. 41| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 8. | | CHAM, K. O.; NOCELLI, R. C. F.; BORGES, L. O.; VIANA-SILVA, F. E. C.; TONELLI, C. A. M.; MALASPINA, O.; MENEZES, C.; ROSA-FONTANA, A. S.; BLOCHTEIN, B.; FREITAS, B. M.; PIRES, C. S. S.; OLIVEIRA, F. F.; CONTRERA, F. A. L.; TOREZANI, K. R. S.; RIBEIRO, M. de F.; SIQUEIRA, M. A. L.; ROCHA, M. C. L. S. A. Pesticide exposure assessment paradigm for stingless bees. Environmental Entomology, v. 48, n. 1, p. 36-48, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Meio Ambiente; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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| Registros recuperados : 8 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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