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Registro Completo |
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Biblioteca(s): |
Embrapa Agricultura Digital. |
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Data corrente: |
28/08/2007 |
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Data da última atualização: |
17/01/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SIMÕES, M.; BAHIA, D.; ZERLOTINI, A.; TORRES, K.; ARTIGUENAVE, F.; NESHICH, G.; KUSER, P.; OLIVEIRA, G. |
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Afiliação: |
MARIANA SIMÕES, Fiocruz; DIANA BAHIA, Fiocruz; ADHEMAR ZERLOTINI, Fiocruz; KLEIDER TORRES, Fiocruz; FRANÇOIS ARTIGUENAVE, Inra; GORAN NESHICH, CNPTIA; PAULA REGINA KUSER FALCAO, CNPTIA; GUILHERME OLIVEIRA, Fiocruz, Santa Casa de Belo Horizonte. |
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Título: |
Single nucleotide polymorphisms identification in expressed genes of Schistosoma mansoni. |
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Ano de publicação: |
2007 |
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Fonte/Imprenta: |
Molecular and Biochemical Parasitology, v. 154, p. 134-140, 2007. |
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DOI: |
10.1016/j.molbiopara.2007.04.003 |
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Idioma: |
Inglês |
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Conteúdo: |
Single nucleotide polymorphism (SNP) markers have been shown to be useful in genetic investigations of medically important parasites and their hosts. In this paper, we describe the prediction and validation of SNPs in ESTs of Schistosoma mansoni. We used 107,417 public sequences of S. mansoni and identified 15,614 high-quality candidate SNPs in 12,184 contigs. The presence of predicted SNPs was observed in well characterized antigens and vaccine candidates such as those coding for myosin; Sm14 and Sm23; cathepsin B and triosephosphate isomerase (TPI). Additionally, SNPs were experimentally validated for the cathepsin B. A comparative model of the S. mansoni cathepsin B was built for predicting the possible consequences of amino acid substitutions on the protein structure. An analysis of the substitutions indicated that the amino acids were mostly located on the surface of the molecule, and we found no evidence for a significant conformational change of the enzyme. However, at least one of the substitutions could result in a structural modification of an epitope |
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Palavras-Chave: |
Bioinformática; Comparative modeling; Computational biology; Polimorfismo de nucleotídeo único. |
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Thesagro: |
Schistosoma Mansoni. |
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Thesaurus Nal: |
Bioinformatics; Cathepsin B; Models; Single nucleotide polymorphism. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02052naa a2200325 a 4500
001 1002699
005 2020-01-17
008 2007 bl uuuu u00u1 u #d
024 7 $a10.1016/j.molbiopara.2007.04.003$2DOI
100 1 $aSIMÕES, M.
245 $aSingle nucleotide polymorphisms identification in expressed genes of Schistosoma mansoni.$h[electronic resource]
260 $c2007
520 $aSingle nucleotide polymorphism (SNP) markers have been shown to be useful in genetic investigations of medically important parasites and their hosts. In this paper, we describe the prediction and validation of SNPs in ESTs of Schistosoma mansoni. We used 107,417 public sequences of S. mansoni and identified 15,614 high-quality candidate SNPs in 12,184 contigs. The presence of predicted SNPs was observed in well characterized antigens and vaccine candidates such as those coding for myosin; Sm14 and Sm23; cathepsin B and triosephosphate isomerase (TPI). Additionally, SNPs were experimentally validated for the cathepsin B. A comparative model of the S. mansoni cathepsin B was built for predicting the possible consequences of amino acid substitutions on the protein structure. An analysis of the substitutions indicated that the amino acids were mostly located on the surface of the molecule, and we found no evidence for a significant conformational change of the enzyme. However, at least one of the substitutions could result in a structural modification of an epitope
650 $aBioinformatics
650 $aCathepsin B
650 $aModels
650 $aSingle nucleotide polymorphism
650 $aSchistosoma Mansoni
653 $aBioinformática
653 $aComparative modeling
653 $aComputational biology
653 $aPolimorfismo de nucleotídeo único
700 1 $aBAHIA, D.
700 1 $aZERLOTINI, A.
700 1 $aTORRES, K.
700 1 $aARTIGUENAVE, F.
700 1 $aNESHICH, G.
700 1 $aKUSER, P.
700 1 $aOLIVEIRA, G.
773 $tMolecular and Biochemical Parasitology$gv. 154, p. 134-140, 2007.
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Embrapa Agricultura Digital (CNPTIA) |
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| Registros recuperados : 3 | |
| 2. |  | SIMÕES, M.; BAHIA, D.; ZERLOTINI, A.; TORRES, K.; ARTIGUENAVE, F.; NESHICH, G.; KUSER, P.; OLIVEIRA, G. Single nucleotide polymorphisms identification in expressed genes of Schistosoma mansoni. Molecular and Biochemical Parasitology, v. 154, p. 134-140, 2007.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Agricultura Digital. |
|    |
| 3. | | SIMÕES, M.; BAHIA, D.; TORRES, K.; ZERLOTINI NETO, A.; ARTIGUENAVE, F.; FALCAO, P. R. K.; NESHICH, G.; OLIVEIRA, G. SNP identification in Schistosoma mansoni expressed genes. In: X-MEETING; INTERNATIONAL CONFERENCE OF THE AB3C, 1., 2005, Caxambu. [Proceedings...]. [S.l.]: Associação Brasileira de Bioinformática e Biologia Computacional, 2005. p. 131. X-meeting 2005. Presented Posters. Na publicação: Paula Kuser.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Agricultura Digital. |
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| Registros recuperados : 3 | |
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