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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
30/04/1997 |
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Data da última atualização: |
19/05/2014 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ARAGÃO, F. J. L.; BARROS, L. M. G.; BRASILEIRO, A. C. M.; RIBEIRO, S. G.; SMITH, F. D.; SANFORD, J. C.; FARIA, J. C.; RECH, E. L. |
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Afiliação: |
FRANCISCO JOSE LIMA ARAGAO, CENARGEN; LEILA MARIA GOMES BARROS, CENARGEN; ANA CRISTINA MIRANDA BRASILEIRO, CENARGEN; SIMONE DA GRACA RIBEIRO, CENARGEN; F. D. SMITH, CORNELL UNIVERSITY; J. C. SANFORD, CORNELL UNIVERSITY; JOSIAS CORREA DE FARIA, CNPAF; ELIBIO LEOPOLDO RECH FILHO, CENARGEN. |
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Título: |
Inheritance of foreign genes in transgenic bean (Phaseolus vulgaris L.) co-transformed via particle bombardment. |
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Ano de publicação: |
1996 |
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Fonte/Imprenta: |
Theoretical and Applied Genetics, Berlin, v. 93, n. 1/2, p. 142-150, July 1996. |
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Idioma: |
Inglês |
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Conteúdo: |
Exploiting the biolistic process we have generated stable transgenic bean (Phaseolus vulgaris L.) plants with unlinked and linked foreign genes. Co-transformation was conducted using plasmid constructions containing a fusion of the gus and neo genes, which were co-introduced with the methionine-rich 2S albumin gene isolated from the Brazil nut and the antisense sequence of AC1, AC2, AC3 and BC1 genes from the bean golden mosaic geminivirus. The results revealed a co-transformation frequency ranging from 40% to 50% when using unlinked genes and 100% for linked genes. The introduced foreign genes were inherited in a Mendelian fashion in most of the transgenic bean lines. PCR and Southern blot hybridization confirmed the integration of the foreign genes in the plant genome. |
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Palavras-Chave: |
Bean; Biolistica; Electrical; Feijão transgênico; Particle; Transgenic bean. |
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Thesagro: |
Feijão; Phaseolus Vulgaris. |
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Thesaurus Nal: |
biolistics. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01696naa a2200313 a 4500
001 1212184
005 2014-05-19
008 1996 bl uuuu u00u1 u #d
100 1 $aARAGÃO, F. J. L.
245 $aInheritance of foreign genes in transgenic bean (Phaseolus vulgaris L.) co-transformed via particle bombardment.$h[electronic resource]
260 $c1996
520 $aExploiting the biolistic process we have generated stable transgenic bean (Phaseolus vulgaris L.) plants with unlinked and linked foreign genes. Co-transformation was conducted using plasmid constructions containing a fusion of the gus and neo genes, which were co-introduced with the methionine-rich 2S albumin gene isolated from the Brazil nut and the antisense sequence of AC1, AC2, AC3 and BC1 genes from the bean golden mosaic geminivirus. The results revealed a co-transformation frequency ranging from 40% to 50% when using unlinked genes and 100% for linked genes. The introduced foreign genes were inherited in a Mendelian fashion in most of the transgenic bean lines. PCR and Southern blot hybridization confirmed the integration of the foreign genes in the plant genome.
650 $abiolistics
650 $aFeijão
650 $aPhaseolus Vulgaris
653 $aBean
653 $aBiolistica
653 $aElectrical
653 $aFeijão transgênico
653 $aParticle
653 $aTransgenic bean
700 1 $aBARROS, L. M. G.
700 1 $aBRASILEIRO, A. C. M.
700 1 $aRIBEIRO, S. G.
700 1 $aSMITH, F. D.
700 1 $aSANFORD, J. C.
700 1 $aFARIA, J. C.
700 1 $aRECH, E. L.
773 $tTheoretical and Applied Genetics, Berlin$gv. 93, n. 1/2, p. 142-150, July 1996.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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