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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
26/03/1999 |
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Data da última atualização: |
09/05/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FARIA, J. C.; SOUZA-DIAS, J. A. C.; SLACK, S. A.; MAXWELL, D. P. |
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Afiliação: |
JOSIAS CORREA DE FARIA, CNPAF; J. A. C. SOUZA-DIAS, IAC; S. A. SLACK, CORNELL UNIVERSITY; D. P. MAXWELL, UNIVERSITY OF WISCONSIN. |
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Título: |
A new geminivirus associated with tomato in the state of Sao Paulo, Brazil. |
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Ano de publicação: |
1997 |
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Fonte/Imprenta: |
Plant Disease, v. 81, n. 4, p. 423, Apr. 1997. |
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DOI: |
https://doi.org/10.1094/PDIS.1997.81.4.423B |
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Idioma: |
Inglês |
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Notas: |
Disease notes. |
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Conteúdo: |
The apical growth of about 20% of young tomato plants in observed fields near Campinas, State of São Paulo, Brazil, had yellow streaking of veins. Leaf symptoms developed into patches of yellow mosaic and the leaves became wavy. The whitefly Bemisia tabaci Genn. transmitted a pathogen from the infected tomato plants to healthy tomato and potato plants, reproducing the original symptoms in tomato. The apical leaves of infected potatoes showed yellow or green mottle that developed into leaf distortion with yellow blotches, symptoms indistinguishable from potato-deforming mosaic disease (2). DNA was extracted from these tomato and potato plants (1). Using DNA from the infected tomato plant, polymerase chain reaction (PCR) with the degenerate primer pair PAC1v1978/ PAV1c715 (1), which amplifies part of the rep gene (AC1 ORF), the common region (CR), and part of the cp gene (AV1 ORF), and with the primer pair PBC1v2039/PBV1c800, which amplifies part of BC1 ORF, CR, and part of BV1 ORF, gave virus-specific DNA fragments of the sizes expected from a whitefly-transmitted geminivirus. These were cloned and the complete nucleotide (sequences for DNA-A (pToYA, GenBank accession no. U79998) and DNA-B (pToYB, GenBank accession no. U80042) fragments obtained. Nucleotide identity between the CRs (184 nucleotides) was 90%, strongly indicating that those fragments correspond to a bipartite subgroup III geminivirus. PCR with the DNA from infected potato gave the expected size fragment for DNA-A. The partial sequence of the rep gene was 100% identical to the homologous sequence from the PCR fragment from the infected tomato. A search in the GenBank, EMBL, DDBJ, and PDB databases, using the BLAST program, found no identical geminivirus. The highest identity for the CR was 75% to tomato mottle geminivirus-Florida (ToMoV) and 74% to bean golden mosaic virus-Brazil. For the rep gene, the highest identity was 73% to tomato yellow leaf curl virus-Israel, an Old World geminivi-rus, followed by 71% to tomato golden mosaic virus-Brazil (TGMV) and ToMoV. For the cp gene, the highest identity was 86% to TGMV, followed by 83% to squash leaf curl geminivirus. Therefore, we propose the name tomato yellow vein streak geminivirus (ToYVSV) for this distinct virus (2). MenosThe apical growth of about 20% of young tomato plants in observed fields near Campinas, State of São Paulo, Brazil, had yellow streaking of veins. Leaf symptoms developed into patches of yellow mosaic and the leaves became wavy. The whitefly Bemisia tabaci Genn. transmitted a pathogen from the infected tomato plants to healthy tomato and potato plants, reproducing the original symptoms in tomato. The apical leaves of infected potatoes showed yellow or green mottle that developed into leaf distortion with yellow blotches, symptoms indistinguishable from potato-deforming mosaic disease (2). DNA was extracted from these tomato and potato plants (1). Using DNA from the infected tomato plant, polymerase chain reaction (PCR) with the degenerate primer pair PAC1v1978/ PAV1c715 (1), which amplifies part of the rep gene (AC1 ORF), the common region (CR), and part of the cp gene (AV1 ORF), and with the primer pair PBC1v2039/PBV1c800, which amplifies part of BC1 ORF, CR, and part of BV1 ORF, gave virus-specific DNA fragments of the sizes expected from a whitefly-transmitted geminivirus. These were cloned and the complete nucleotide (sequences for DNA-A (pToYA, GenBank accession no. U79998) and DNA-B (pToYB, GenBank accession no. U80042) fragments obtained. Nucleotide identity between the CRs (184 nucleotides) was 90%, strongly indicating that those fragments correspond to a bipartite subgroup III geminivirus. PCR with the DNA from infected potato gave the expected size fragment for DNA... Mostrar Tudo |
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Palavras-Chave: |
Geminivirus; São Paulo. |
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Thesagro: |
Bemisia tabaci; Doença de planta; Tomate. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 02953naa a2200241 a 4500
001 1205669
005 2022-05-09
008 1997 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1094/PDIS.1997.81.4.423B$2DOI
100 1 $aFARIA, J. C.
245 $aA new geminivirus associated with tomato in the state of Sao Paulo, Brazil.
260 $c1997
500 $aDisease notes.
520 $aThe apical growth of about 20% of young tomato plants in observed fields near Campinas, State of São Paulo, Brazil, had yellow streaking of veins. Leaf symptoms developed into patches of yellow mosaic and the leaves became wavy. The whitefly Bemisia tabaci Genn. transmitted a pathogen from the infected tomato plants to healthy tomato and potato plants, reproducing the original symptoms in tomato. The apical leaves of infected potatoes showed yellow or green mottle that developed into leaf distortion with yellow blotches, symptoms indistinguishable from potato-deforming mosaic disease (2). DNA was extracted from these tomato and potato plants (1). Using DNA from the infected tomato plant, polymerase chain reaction (PCR) with the degenerate primer pair PAC1v1978/ PAV1c715 (1), which amplifies part of the rep gene (AC1 ORF), the common region (CR), and part of the cp gene (AV1 ORF), and with the primer pair PBC1v2039/PBV1c800, which amplifies part of BC1 ORF, CR, and part of BV1 ORF, gave virus-specific DNA fragments of the sizes expected from a whitefly-transmitted geminivirus. These were cloned and the complete nucleotide (sequences for DNA-A (pToYA, GenBank accession no. U79998) and DNA-B (pToYB, GenBank accession no. U80042) fragments obtained. Nucleotide identity between the CRs (184 nucleotides) was 90%, strongly indicating that those fragments correspond to a bipartite subgroup III geminivirus. PCR with the DNA from infected potato gave the expected size fragment for DNA-A. The partial sequence of the rep gene was 100% identical to the homologous sequence from the PCR fragment from the infected tomato. A search in the GenBank, EMBL, DDBJ, and PDB databases, using the BLAST program, found no identical geminivirus. The highest identity for the CR was 75% to tomato mottle geminivirus-Florida (ToMoV) and 74% to bean golden mosaic virus-Brazil. For the rep gene, the highest identity was 73% to tomato yellow leaf curl virus-Israel, an Old World geminivi-rus, followed by 71% to tomato golden mosaic virus-Brazil (TGMV) and ToMoV. For the cp gene, the highest identity was 86% to TGMV, followed by 83% to squash leaf curl geminivirus. Therefore, we propose the name tomato yellow vein streak geminivirus (ToYVSV) for this distinct virus (2).
650 $aBemisia tabaci
650 $aDoença de planta
650 $aTomate
653 $aGeminivirus
653 $aSão Paulo
700 1 $aSOUZA-DIAS, J. A. C.
700 1 $aSLACK, S. A.
700 1 $aMAXWELL, D. P.
773 $tPlant Disease$gv. 81, n. 4, p. 423, Apr. 1997.
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Embrapa Arroz e Feijão (CNPAF) |
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| 1. |  | COSTA, M. G. C.; GONÇALVES, L. P.; MARTINS, C. P. S.; PEDROSA, A. M.; PEREIRA, S. L. S.; OLIVEIRA, T. M.; CIDADE, L. C.; CAMILLO, L. R.; GESTEIRA, A. da S.; COELHO FILHO, M. A.; SOARES FILHO, W. dos S. Transcriptional regulation of drought response and tolerance in citrus and strategies for development of drought-tolerant transgenic rootstocks. In: INTERNATIONAL CITRUS CONGRESS, 2016, Foz do Iguaçu. Abstract book. Campinas: IAC; Londrina: IAPAR, 2016. p.149. S14-56.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 2. | | COSTA, M. G. C.; CIDADE, L. C.; OLIVEIRA, T. M. de; MARTINS, C. P. S.; MENDES, A. F. S.; PEREIRA, S. L. S.; PEDROSA, A. M.; GESTEIRA, A. da S.; GIRARDI, E. A.; COELHO FILHO, M. A.; SOARES FILHO, W. dos S.; MACHADO, M. A. Characterization of genes associated with two agronomically important traits in citrus: drought tolerance and fruit color. In: INTERNATIONAL CITRUS CONGRESS, 22., 2012.Valencia. Book of abstracts. Valencia: International Society of Citriculture; Instituto Valenciano de Investigaciones Agrarias; Fundación Agroalimed, 2012. Documento eletrônico.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| Registros recuperados : 2 | |
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