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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
02/04/2004 |
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Data da última atualização: |
04/05/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BRONDANI, C.; RANGEL, P. H. N.; BORBA, T. C. O.; BRONDANI, R. P. V. |
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Afiliação: |
CLAUDIO BRONDANI, CNPAF; PAULO HIDEO NAKANO RANGEL, CNPAF; TEREZA CRISTINA DE OLIVEIRA BORBA, CNPAF; ROSANA PEREIRA VIANELLO, CNPAF. |
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Título: |
Transferability of microsatellite and sequence tagged site markers in Oryza species. |
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Ano de publicação: |
2003 |
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Fonte/Imprenta: |
Hereditas, v. 138, p. 187-192, 2003. |
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DOI: |
10.1034/j.1601-5223.2003.01656.x |
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Idioma: |
Inglês |
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Conteúdo: |
The genus Oryza comprises 22 species which are potentially useful as a source of genetic variability that can be introgressed into the worldwide cultivated rice, Oryza sativa. Molecular markers are useful tools for monitoring gene introgressions and for detecting polymorphism among species. In this study, cross-amplification was estimated among 28 accessions of 16 Oryza species, representing the genomes AA, BB, CC, BBCC and CCDD, using 59 microsatellite (OG, OS and RM series) and 15 STS (Sequence Tagged Sites) markers. All markers amplified at least one Oryza species, indicating different levels of transferability across species. Markers based on microsatellite sequences amplified 37 % of the accessions, with an average of 6.58 alleles per locus and an average polymorphism information content (PIC) of 70 %. For STS markers, the amplification level was 53.3 %, and the average number of alleles and PIC values were 1.6 and 10 %, respectively. These Results showed that although the STS markers detected a reduced level of genetic diversity, the transferability was higher, indicating that they can be used for genetic analysis when evaluating less genetically related species of Oryza. Among the microsatellite markers, an analysis of species with an AA genome showed that the OG markers produced the highest level of polymorphic loci (54.6 %), followed by RM markers (48 %). Highly polymorphic and transferable molecular markers in Oryza can be useful for exploiting the genetic resources of this genus, for detecting allelic variants in loci associated with important agronomic traits, and for monitoring alleles introgressed from wild relatives to cultivated rice. MenosThe genus Oryza comprises 22 species which are potentially useful as a source of genetic variability that can be introgressed into the worldwide cultivated rice, Oryza sativa. Molecular markers are useful tools for monitoring gene introgressions and for detecting polymorphism among species. In this study, cross-amplification was estimated among 28 accessions of 16 Oryza species, representing the genomes AA, BB, CC, BBCC and CCDD, using 59 microsatellite (OG, OS and RM series) and 15 STS (Sequence Tagged Sites) markers. All markers amplified at least one Oryza species, indicating different levels of transferability across species. Markers based on microsatellite sequences amplified 37 % of the accessions, with an average of 6.58 alleles per locus and an average polymorphism information content (PIC) of 70 %. For STS markers, the amplification level was 53.3 %, and the average number of alleles and PIC values were 1.6 and 10 %, respectively. These Results showed that although the STS markers detected a reduced level of genetic diversity, the transferability was higher, indicating that they can be used for genetic analysis when evaluating less genetically related species of Oryza. Among the microsatellite markers, an analysis of species with an AA genome showed that the OG markers produced the highest level of polymorphic loci (54.6 %), followed by RM markers (48 %). Highly polymorphic and transferable molecular markers in Oryza can be useful for exploiting the genetic resource... Mostrar Tudo |
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Palavras-Chave: |
Microsatellite. |
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Thesagro: |
Arroz; Marcador Molecular; Oryza Sativa. |
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Thesaurus Nal: |
Genetic markers; Microsatellite repeats; Rice. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02407naa a2200253 a 4500
001 1205061
005 2022-05-04
008 2003 bl uuuu u00u1 u #d
024 7 $a10.1034/j.1601-5223.2003.01656.x$2DOI
100 1 $aBRONDANI, C.
245 $aTransferability of microsatellite and sequence tagged site markers in Oryza species.$h[electronic resource]
260 $c2003
520 $aThe genus Oryza comprises 22 species which are potentially useful as a source of genetic variability that can be introgressed into the worldwide cultivated rice, Oryza sativa. Molecular markers are useful tools for monitoring gene introgressions and for detecting polymorphism among species. In this study, cross-amplification was estimated among 28 accessions of 16 Oryza species, representing the genomes AA, BB, CC, BBCC and CCDD, using 59 microsatellite (OG, OS and RM series) and 15 STS (Sequence Tagged Sites) markers. All markers amplified at least one Oryza species, indicating different levels of transferability across species. Markers based on microsatellite sequences amplified 37 % of the accessions, with an average of 6.58 alleles per locus and an average polymorphism information content (PIC) of 70 %. For STS markers, the amplification level was 53.3 %, and the average number of alleles and PIC values were 1.6 and 10 %, respectively. These Results showed that although the STS markers detected a reduced level of genetic diversity, the transferability was higher, indicating that they can be used for genetic analysis when evaluating less genetically related species of Oryza. Among the microsatellite markers, an analysis of species with an AA genome showed that the OG markers produced the highest level of polymorphic loci (54.6 %), followed by RM markers (48 %). Highly polymorphic and transferable molecular markers in Oryza can be useful for exploiting the genetic resources of this genus, for detecting allelic variants in loci associated with important agronomic traits, and for monitoring alleles introgressed from wild relatives to cultivated rice.
650 $aGenetic markers
650 $aMicrosatellite repeats
650 $aRice
650 $aArroz
650 $aMarcador Molecular
650 $aOryza Sativa
653 $aMicrosatellite
700 1 $aRANGEL, P. H. N.
700 1 $aBORBA, T. C. O.
700 1 $aBRONDANI, R. P. V.
773 $tHereditas$gv. 138, p. 187-192, 2003.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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| 3. |  | STENIO, K.; VILLAS-BOAS, P. R.; MILORI, D. M. B. P. Projeto e construção de um protótipo de LIBS de bancada compacto para análises agroambientais. In: SIMPÓSIO NACIONAL DE INSTRUMENTAÇÃO AGROPECUÁRIA, 4., 2019, São Carlos, SP. Ciência, inovação e mercado: anais. São Carlos, SP: Embrapa Instrumentação, 2019. Editores: Paulino Ribeiro Villas-Boas, Maria Alice Martins, Débora Marcondes Bastos Pereira Milori, Ladislau Martin Neto. SIAGRO 2019. 185-189| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação. |
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| 4. | | CERNA, M.; BECKER, M.; MILORI, D. M. B. P.; STÊNIO, K.; CAMPOS, M.; VILLAS-BOAS, P. R.; MAGALHÃES, D. Plataforma robótica móvel para espectroscopia LIBS em ambiente agrícola. In: WORKSHOP SOBRE LASER EM QUÍMICA ANALÍTICA, 2., 2015, São Carlos. Livro de resumos... São Carlos: UFSCar; Embrapa Instrumentação, 2015. p. 39.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação. |
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| 5. | | GUEDES, W. N.; BABOS, D. V.; COSTA, V. C.; MORAIS, C. P de; FREITAS, V. S.; STENIO, K.; XAVIER, A. A. P.; BORDUCHI, L. C. L.; VILLAS-BOAS, P. R.; MILORI, D. M. B. P. Evaluation of univariate and multivariate calibration strategies for the direct determination of total carbon in soils by laser-induced breakdown spectroscopy: tutorial. Journal of the Optical Society of America B, v. 40, n. 5, 2023. 1319 - 1330| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 3 |
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| Registros recuperados : 5 | |
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