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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
28/11/1994 |
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Data da última atualização: |
17/04/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MAGALHÃES, B. P.; HUMBER, R. A.; SHIELDS, E. J.; ROBERTS, D. W. |
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Afiliação: |
BONIFACIO PEIXOTO MAGALHAES, CNPAF. |
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Título: |
Effects of environment and nutrition on conidium germination and appressorium formation by Zoophthora radicans (Zygomycetes: entomophthorales): a pathogen of the potato leafhopper (Homoptera: cicadellidae). |
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Ano de publicação: |
1991 |
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Fonte/Imprenta: |
Environmental Entomology, v. 20, n. 5, p. 1460-1468, Oct. 1991. |
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DOI: |
10.1093/ee/20.5.1460 |
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Idioma: |
Inglês |
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Conteúdo: |
Conidium germination and differentiation of Zoophthora radicans on water agar were investigated to identify requirements for the production of appressoria, the structures needed for virulence. Appressorium formation was more sensitive to environmental and nutritional changes than was conidium germination. Temperature affected both formation of germ tubes and appressoria, and the best temperature-nutrient interaction was found for appressorium formation at 25-30°C and 1% yeast extract. At 15°C, conidium germination was depressed independent of the nutrient concentration, and appressoria did not form. The volume of liquid overlaying the conidia and the yeast extract concentration individually affected appressorium formation. The best interaction between the volume of liquid and yeast extract concentration was the combination of a large volume of liquid medium (3 ml/962 mm 2 surface area) and 1% yeast extract. Of the 12 nitrogen sources tested, the best for germination and appressorium formation were yeast extract and Bacto-Soytone. Glucose, maltose, and starch were the best of the 12 carbon sources tested. Increasing osmotic pressure caused by different concentrations of maltose and polyethylene glycol adversely affected formation of germ tubes and appressoria. Subculturing did not affect formation of germ tubes, but the frequency of appressorium formation decreased after 12 transfers. The best pH for appressorium formation was between 7.0 and 7.2. Z. radicans produced appressoria on cuticles of dead Empoasca fabae (Harris) nymphs at 25°C in the dark. MenosConidium germination and differentiation of Zoophthora radicans on water agar were investigated to identify requirements for the production of appressoria, the structures needed for virulence. Appressorium formation was more sensitive to environmental and nutritional changes than was conidium germination. Temperature affected both formation of germ tubes and appressoria, and the best temperature-nutrient interaction was found for appressorium formation at 25-30°C and 1% yeast extract. At 15°C, conidium germination was depressed independent of the nutrient concentration, and appressoria did not form. The volume of liquid overlaying the conidia and the yeast extract concentration individually affected appressorium formation. The best interaction between the volume of liquid and yeast extract concentration was the combination of a large volume of liquid medium (3 ml/962 mm 2 surface area) and 1% yeast extract. Of the 12 nitrogen sources tested, the best for germination and appressorium formation were yeast extract and Bacto-Soytone. Glucose, maltose, and starch were the best of the 12 carbon sources tested. Increasing osmotic pressure caused by different concentrations of maltose and polyethylene glycol adversely affected formation of germ tubes and appressoria. Subculturing did not affect formation of germ tubes, but the frequency of appressorium formation decreased after 12 transfers. The best pH for appressorium formation was between 7.0 and 7.2. Z. radicans produced appress... Mostrar Tudo |
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Palavras-Chave: |
Conidium; Grasshopper. |
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Thesagro: |
Conídio; Empoasca Fabae; Fungo; Gafanhoto; Germinação; Zoophthora Radicans. |
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Thesaurus Nal: |
Empoasca; germination; Insect pathology; Insects. |
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Categoria do assunto: |
--
O Insetos e Entomologia |
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Marc: |
LEADER 02564naa a2200313 a 4500
001 1198110
005 2020-04-17
008 1991 bl uuuu u00u1 u #d
024 7 $a10.1093/ee/20.5.1460$2DOI
100 1 $aMAGALHÃES, B. P.
245 $aEffects of environment and nutrition on conidium germination and appressorium formation by Zoophthora radicans (Zygomycetes$bentomophthorales): a pathogen of the potato leafhopper (Homoptera: cicadellidae).$h[electronic resource]
260 $c1991
520 $aConidium germination and differentiation of Zoophthora radicans on water agar were investigated to identify requirements for the production of appressoria, the structures needed for virulence. Appressorium formation was more sensitive to environmental and nutritional changes than was conidium germination. Temperature affected both formation of germ tubes and appressoria, and the best temperature-nutrient interaction was found for appressorium formation at 25-30°C and 1% yeast extract. At 15°C, conidium germination was depressed independent of the nutrient concentration, and appressoria did not form. The volume of liquid overlaying the conidia and the yeast extract concentration individually affected appressorium formation. The best interaction between the volume of liquid and yeast extract concentration was the combination of a large volume of liquid medium (3 ml/962 mm 2 surface area) and 1% yeast extract. Of the 12 nitrogen sources tested, the best for germination and appressorium formation were yeast extract and Bacto-Soytone. Glucose, maltose, and starch were the best of the 12 carbon sources tested. Increasing osmotic pressure caused by different concentrations of maltose and polyethylene glycol adversely affected formation of germ tubes and appressoria. Subculturing did not affect formation of germ tubes, but the frequency of appressorium formation decreased after 12 transfers. The best pH for appressorium formation was between 7.0 and 7.2. Z. radicans produced appressoria on cuticles of dead Empoasca fabae (Harris) nymphs at 25°C in the dark.
650 $aEmpoasca
650 $agermination
650 $aInsect pathology
650 $aInsects
650 $aConídio
650 $aEmpoasca Fabae
650 $aFungo
650 $aGafanhoto
650 $aGerminação
650 $aZoophthora Radicans
653 $aConidium
653 $aGrasshopper
700 1 $aHUMBER, R. A.
700 1 $aSHIELDS, E. J.
700 1 $aROBERTS, D. W.
773 $tEnvironmental Entomology$gv. 20, n. 5, p. 1460-1468, Oct. 1991.
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Embrapa Arroz e Feijão (CNPAF) |
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| 16. |  | GOMES, J. C. C.; AQUINI, D.; GOMES, F. R. C.; STUMPF JUNIOR, W. Da difusão de tecnologia ao desenvolvimento sustentável: trajetória da transferência de tecnologia na Embrapa Clima Temperado. Cadernos de Ciência & Tecnologia, Brasília, DF, v. 28, n. 1, p. 159-188, jan./abr. 2011.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
| Biblioteca(s): Embrapa Unidades Centrais. |
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| 19. | | ZANELA, M. B.; RIBEIRO, M. E. R.; FISCHER, V.; GOMES, J. F.; STUMPF JUNIOR, W. Ocorrência do leite instável não ácido no noroeste de Rio Grande do Sul. Arquivo Brasileiro de Medicina Veterinaria e Zootecnia, Belo Horizonte, v.61, n.4, p.1009-1013, 2009| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Clima Temperado. |
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| Registros recuperados : 48 | |
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