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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
28/11/1994 |
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Data da última atualização: |
26/10/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MAGALHÃES, B. P.; WAYNE, R.; HUMBER, R. A.; SHIELDS, E. J.; ROBERTS, D. W. |
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Afiliação: |
BONIFACIO PEIXOTO MAGALHAES, CNPAF. |
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Título: |
Calcium-regulated appressorium formation of the entomopathogenic fungus Zoophthora radicans. |
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Ano de publicação: |
1991 |
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Fonte/Imprenta: |
Protoplasma, v. 160, p. 77-88, June 1991. |
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DOI: |
https://doi.org/10.1007/BF01539959 |
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Idioma: |
Inglês |
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Conteúdo: |
The fungus Zoophthora radicans (Zygomycetes: Entomophthorales) requires external Ca2+ for appressorium formation but not for conidial germination. The number of appressoria formed depends on the Ca2+ concentration of the medium. At low [Ca2+] (100 pM) nuclear division and germ tube growth are significantly reduced compared to higher Ca2+ concentrations (10 and 1,000 uM). By contrast, neither external K+ nor external Cl- is needed for germination or appressorium formation. Treatment of conidia with a Ca2+-antagonist, Nd3+, and a Ca2+-channel blocker, nifedipine, inhibits appressorium formation, showing that a Ca2+ influx is required for appressorium formation. Furthermore, the partial yet saturating inhibition by nifedipine and complete inhibition by Nd3+ indicates that at least two kinds of Ca2+ channels are involved in appressorium formation. A contribution of intracellular Ca2+ to the signal transduction chain for the formation of appressoria is demonstrated by the inhibitory effect of the intracellular Ca2+ antagonist TMB-8. The calmodulin antagonists R24571, TFP, W-7, and W-5 inhibit appressorium formation at concentrations which have no effect on germination. The data presented in this paper are consistent with the hypothesis that a Ca2+/calmodulin system is involved in regulating appressorium formation. However, since the direct effects of the drugs were not specifically tested on their proposed binding sites, we leave room for alternative hypotheses that have yet to be formulated. MenosThe fungus Zoophthora radicans (Zygomycetes: Entomophthorales) requires external Ca2+ for appressorium formation but not for conidial germination. The number of appressoria formed depends on the Ca2+ concentration of the medium. At low [Ca2+] (100 pM) nuclear division and germ tube growth are significantly reduced compared to higher Ca2+ concentrations (10 and 1,000 uM). By contrast, neither external K+ nor external Cl- is needed for germination or appressorium formation. Treatment of conidia with a Ca2+-antagonist, Nd3+, and a Ca2+-channel blocker, nifedipine, inhibits appressorium formation, showing that a Ca2+ influx is required for appressorium formation. Furthermore, the partial yet saturating inhibition by nifedipine and complete inhibition by Nd3+ indicates that at least two kinds of Ca2+ channels are involved in appressorium formation. A contribution of intracellular Ca2+ to the signal transduction chain for the formation of appressoria is demonstrated by the inhibitory effect of the intracellular Ca2+ antagonist TMB-8. The calmodulin antagonists R24571, TFP, W-7, and W-5 inhibit appressorium formation at concentrations which have no effect on germination. The data presented in this paper are consistent with the hypothesis that a Ca2+/calmodulin system is involved in regulating appressorium formation. However, since the direct effects of the drugs were not specifically tested on their proposed binding sites, we leave room for alternative hypotheses that have yet to b... Mostrar Tudo |
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Palavras-Chave: |
Appressorium formation; Entomopathogen. |
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Thesagro: |
Fungo; Fungo Entomógeno; Zoophthora Radicans. |
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Thesaurus Nal: |
Calcium; Calmodulin; Entomopathogenic fungi. |
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Categoria do assunto: |
O Insetos e Entomologia |
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Marc: |
LEADER 02322naa a2200277 a 4500
001 1198109
005 2021-10-26
008 1991 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/BF01539959$2DOI
100 1 $aMAGALHÃES, B. P.
245 $aCalcium-regulated appressorium formation of the entomopathogenic fungus Zoophthora radicans.$h[electronic resource]
260 $c1991
520 $aThe fungus Zoophthora radicans (Zygomycetes: Entomophthorales) requires external Ca2+ for appressorium formation but not for conidial germination. The number of appressoria formed depends on the Ca2+ concentration of the medium. At low [Ca2+] (100 pM) nuclear division and germ tube growth are significantly reduced compared to higher Ca2+ concentrations (10 and 1,000 uM). By contrast, neither external K+ nor external Cl- is needed for germination or appressorium formation. Treatment of conidia with a Ca2+-antagonist, Nd3+, and a Ca2+-channel blocker, nifedipine, inhibits appressorium formation, showing that a Ca2+ influx is required for appressorium formation. Furthermore, the partial yet saturating inhibition by nifedipine and complete inhibition by Nd3+ indicates that at least two kinds of Ca2+ channels are involved in appressorium formation. A contribution of intracellular Ca2+ to the signal transduction chain for the formation of appressoria is demonstrated by the inhibitory effect of the intracellular Ca2+ antagonist TMB-8. The calmodulin antagonists R24571, TFP, W-7, and W-5 inhibit appressorium formation at concentrations which have no effect on germination. The data presented in this paper are consistent with the hypothesis that a Ca2+/calmodulin system is involved in regulating appressorium formation. However, since the direct effects of the drugs were not specifically tested on their proposed binding sites, we leave room for alternative hypotheses that have yet to be formulated.
650 $aCalcium
650 $aCalmodulin
650 $aEntomopathogenic fungi
650 $aFungo
650 $aFungo Entomógeno
650 $aZoophthora Radicans
653 $aAppressorium formation
653 $aEntomopathogen
700 1 $aWAYNE, R.
700 1 $aHUMBER, R. A.
700 1 $aSHIELDS, E. J.
700 1 $aROBERTS, D. W.
773 $tProtoplasma$gv. 160, p. 77-88, June 1991.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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| Registros recuperados : 23 | |
| 6. |  | BARROS, N. N.; KAWAS, J. R.; JOHNSON, W. L.; SHELTON, J. M.; SOUZA NETO, J. de. Influência da energia do desempenho de cordeiros Somalis alimentados com capim elefante (Pennisetum purpureum). In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 25., 1988, Viçosa, MG. Anais... Viçosa, MG: Sociedade Brasileira de Zootecnia, 1988. p. 114.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 8. | | KAWAS, J. R.; LOPES, J.; DANELON, D. L.; LU, C. D. Influence of forage-to-concentrate ratios on intake, digestibility, chewing and milk production of dairy goats. Small Ruminant Research, v. 4, n. 1, p. 11-18, 1991.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 9. | | SCHACHT, W. H.; KAWAS, J. R.; MALECHEK, J. C.; FREIRE, L. C. L. Efeito da suplementação de energia e nitrogênio no ganho de peso de caprinos em pastagem nativa (caatinga) na época seca, no nordeste do Brasil. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 22., 1985, Balneário Camboriu, SC. Anais... Balneário Camboriú: Sociedade Brasileira de Zootecnia, 1985. p. 62.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 10. | | SILVA, F. L. R. da; ARRUDA, F. de A. V.; KAWAS, J. R. Efeito do sombreamento sobre o consumo de alimento e água, em ovinos da raça Santa Inês Vermelha do Nordeste. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 25., 1988, Viçosa, MG. Anais... Viçosa, MG: Sociedade Brasileira de Zootecnia, 1988. p. 302.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 12. | | KAWAS, J. R.; GUIMARAES, W.; BARROS, N. N.; FREIRE, L. C. L.; FOOTE, W. C. Survival and growth of goat kids grazing native range from dams supplemented with incremental energy levels. In: INTERNATIONAL CONFERENCE ON GOATS, 4., 1987, Brasília, DF. Proceedings... Brasília, DF: EMBRAPA-DDT, 1987. p. 1399. Abstract 139.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 13. | | KAWAS, J. R.; GUIMARAES, W.; BARROS, N. N.; SANCHEZ, O.; SHELTON, J. M.; JOHNSON, W. L. Interrelação energia-proteína no crescimento de ovinos deslanados. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 23., 1986, Campo Grande. Anais... Campo Grande: Sociedade Brasileira de Zootecnia, 1986. p. 95.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 14. | | BARROS, N. N.; KAWAS, J. R.; LOPES, E. A.; JOHNSON, W. L. Estudo comparativo da digestibilidade de leguminosa nativa com caprinos e ovinos, no semi-árido do Estado do Ceará. II. Digestibilidade in vivo da silagem de mata-pasto (Cassia ssp). Pesquisa Agropecuária Brasileira, Brasília, DF, v. 27, n. 11, p. 1551-1555, nov., 1992.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 15. | | LU, C. D.; OSBORN, R. G.; KAWAS, J. R.; ARRUDA, F. Prediction of dry matter intake in lactating and growing dairy goats. In: INTERNATIONAL CONFERENCE ON GOATS, 4., 1987, Brasília, DF. Proceedings... Brasília, DF: EMBRAPA-DDT, 1987. p. 1386. Resumo 121.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 16. | | BARROS, N. N.; OLIVEIRA, E. R.; ROBB, T. W.; ARRUDA, F. A. V.; KAWAS, J. R.; JOHNSON, W. L. Acabamento de caprinos e ovinos durante a época seca, no Nordeste. In: REUNIÃO TÉCNICO-CIENTÍFICA DO PROGRAMA DE APOIO A PESQUISA COLABORATIVA DE PEQUENOS RUMINANTES, 1., 1986. Sobral. Anais... Sobral: EMBRAPA-CNPC; SR-CRSP, 1986. p. 285-295. (EMBRAPA-CNPC. Documentos, 6).| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 17. | | ARRUDA, F. A. V.; BEZERRA, R.; RIBEIRO, F. L.; SANTOS, J. W. dos; KAWAS, J. R.; SHELTON, J. M. The interaction of environmental variables (time of day and shade vs sun) and level of nutrition on animal response (body temperature, respiratory rate, water intake and weight gains) with hair sheep. In: REUNIÃO TÉCNICO-CIENTÍFICA DO PROGRAMA DE APOIO À PESQUISA COLABORATIVA DE PEQUENOS RUMINANTES, 1., 1986, Sobral. Caprinos e ovinos no Nordeste do Brasil: anais. Sobral: EMBRAPA-CNPC: SR-CRSP, 1986. p. 259-265. (EMBRAPA-CNPC. Documentos, 6).| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 18. | | BARROS, N. N.; KAWAS, J. R.; FREIRE, L. C. L.; JOHNSON, W. L.; SANTOS, J. W. dos. Nutritive value of total mixed rations with various energy sources fed to lambs, in Northeast Brazil. Journal of Animal Science, v. 61, suppl.1, p. 452, 1985. Abstract 592. Abstracts of the 77 ASAS Annual Meeting, Biloxi, Mississipi, 1985.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 19. | | KAWAS, J. R.; CARNEIRO, H.; BARROS, N. N.; FREIRE, L. C. L.; KAWAS, F. N.; SHELTON, M.; JOHNSON, W. L. Valor nutritivo para caprinos das silagens de sorgo forrageiro (Sorghum vulgare) e de cunhã (Clitoria ternatea) em dois estágios de maturidade. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 22., 1985, Balneário Camboriú, SC. Anais... Balneário Camboriú: Sociedade Brasileira de Zootecnia, 1985. p. 355.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 20. | | KAWAS, J. R.; CARNEIRO, H.; BEZERRA, M.; ARRUDA, F. de A. V.; FREIRE, L. C. L.; JOHNSON, W. C.; SHELTON, J. M. Influence of fiber component of Clitoria ternatea and sorghum silages on intake and digestion by goats in Northeast Brazil. Journal of Animal Science, v. 61, supl. 1, p. 475-476, 1985. Abstract 648. Abstracts of the 77 ASAS Annual Meeting, Biloxi, Mississipi, 1985.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| Registros recuperados : 23 | |
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