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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
24/08/2005 |
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Data da última atualização: |
25/09/2024 |
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Autoria: |
SA, M. F. G. de; CHRISPEELS, M. J. |
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Título: |
Molecular cloning of bruchid (Zabrotes subfasciatus) alpha-amylase cDNA and interactions of the expressed enzyme with bean amylase inhibitors. |
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Ano de publicação: |
1997 |
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Fonte/Imprenta: |
Insect Biochemistry and Molecular Biology, v. 27, n. 4, p. 271-281, 1997. |
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Idioma: |
Inglês |
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Conteúdo: |
Introduction; Materials and methods: Insects, RNA extraction, Northern blot analysis, Larval extraction, Reverse transcript-polymerase chain reaction and cDNA cloning, Cells plasmid, recombinant virus and insect cell transfection, Analysis of expressed Zs alfa-amylase and amylase activity detection, Protein assay, Binding ability of the inhibitor (alfaAI-2) to expressed Zs alfa-amylase; Results: Cloning of the ZsAmy cDNA, Analysis of the transcript, ZsAmy encodes and active amylase, Inhibition of Zs-amylase by bean alfa-amylase inhibitiors, at 30°C, ZsAmy forms a complex with alfaAI-2, but not with alfaAI-1. Discussion. |
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Thesagro: |
Caruncho; Clonagem; Feijão; Phaseolus Vulgaris; Zabrotes Subfasciatus. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01273naa a2200193 a 4500
001 1185994
005 2024-09-25
008 1997 bl uuuu u00u1 u #d
100 1 $aSA, M. F. G. de
245 $aMolecular cloning of bruchid (Zabrotes subfasciatus) alpha-amylase cDNA and interactions of the expressed enzyme with bean amylase inhibitors.$h[electronic resource]
260 $c1997
520 $aIntroduction; Materials and methods: Insects, RNA extraction, Northern blot analysis, Larval extraction, Reverse transcript-polymerase chain reaction and cDNA cloning, Cells plasmid, recombinant virus and insect cell transfection, Analysis of expressed Zs alfa-amylase and amylase activity detection, Protein assay, Binding ability of the inhibitor (alfaAI-2) to expressed Zs alfa-amylase; Results: Cloning of the ZsAmy cDNA, Analysis of the transcript, ZsAmy encodes and active amylase, Inhibition of Zs-amylase by bean alfa-amylase inhibitiors, at 30°C, ZsAmy forms a complex with alfaAI-2, but not with alfaAI-1. Discussion.
650 $aCaruncho
650 $aClonagem
650 $aFeijão
650 $aPhaseolus Vulgaris
650 $aZabrotes Subfasciatus
700 1 $aCHRISPEELS, M. J.
773 $tInsect Biochemistry and Molecular Biology$gv. 27, n. 4, p. 271-281, 1997.
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