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 | Acesso ao texto completo restrito à biblioteca da Embrapa Meio Ambiente. Para informações adicionais entre em contato com cnpma.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
01/04/1998 |
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Data da última atualização: |
18/04/2017 |
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Autoria: |
SILVEIRA, F. Q. de P.; MELO, I. S. de; FERREIRA FILHO, E. X. |
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Afiliação: |
FABIANE QUIRINO DE PAULA SILVEIRA, UnB/DF; ITAMAR SOARES DE MELO, CNPMA; EDIVALDO XIMENES FERREIRA FILHO, UnB. |
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Título: |
Carbohydrate-hydrolysing enzyme activity production by solid-state cultures of Trichoderma harzianum strains. |
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Ano de publicação: |
1997 |
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Fonte/Imprenta: |
Revista de Microbiologia, v.28, n.3 p.152-156, 1997. |
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Idioma: |
Inglês |
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Conteúdo: |
Screening of nine Trichoderma harzianum strains grown by solid-state fermentation on media containing wheat bran as the carbon source demonstrated that different carbohydrase activities were produced by most of the strains investigated. B-Glucosidase, B-xylosidase, pectinase and a-amylase activities were produced by all strains. a-Amylase, B-xylanase and B-glucosidase activities were the best yields obtained. B-Mannosidase, a-glucuronidase, carboxymethyl-cellulase and FPase activities were poorly produced. The best balance of enzyme activities was obtained from cultures of T.harzianum strains 4 and 8. The procedure for enzyme extraction (25 mM sodium acetate buffer, pH 5.0) of T.harzianum strain C was compared with a second method using the above buffer sol ution containing 0.1% tween 80 plus blending in a homogenizer. The results indicated that the best yield of specific activity was obtained from the former extraction procedure. Furthermore, the protein content obtained by using this extraction procedure was very low. Carboxymethyl-cellulase activity was only detected when the second extraction procedure was used. |
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Palavras-Chave: |
Cell wall; Enzyme production; Protein determination; Solid-state cultures. |
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Thesaurus Nal: |
culture media; electrophoresis; extraction; strains. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01866naa a2200241 a 4500
001 1012785
005 2017-04-18
008 1997 bl uuuu u00u1 u #d
100 1 $aSILVEIRA, F. Q. de P.
245 $aCarbohydrate-hydrolysing enzyme activity production by solid-state cultures of Trichoderma harzianum strains.$h[electronic resource]
260 $c1997
520 $aScreening of nine Trichoderma harzianum strains grown by solid-state fermentation on media containing wheat bran as the carbon source demonstrated that different carbohydrase activities were produced by most of the strains investigated. B-Glucosidase, B-xylosidase, pectinase and a-amylase activities were produced by all strains. a-Amylase, B-xylanase and B-glucosidase activities were the best yields obtained. B-Mannosidase, a-glucuronidase, carboxymethyl-cellulase and FPase activities were poorly produced. The best balance of enzyme activities was obtained from cultures of T.harzianum strains 4 and 8. The procedure for enzyme extraction (25 mM sodium acetate buffer, pH 5.0) of T.harzianum strain C was compared with a second method using the above buffer sol ution containing 0.1% tween 80 plus blending in a homogenizer. The results indicated that the best yield of specific activity was obtained from the former extraction procedure. Furthermore, the protein content obtained by using this extraction procedure was very low. Carboxymethyl-cellulase activity was only detected when the second extraction procedure was used.
650 $aculture media
650 $aelectrophoresis
650 $aextraction
650 $astrains
653 $aCell wall
653 $aEnzyme production
653 $aProtein determination
653 $aSolid-state cultures
700 1 $aMELO, I. S. de
700 1 $aFERREIRA FILHO, E. X.
773 $tRevista de Microbiologia$gv.28, n.3 p.152-156, 1997.
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