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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
08/06/2026 |
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Data da última atualização: |
08/06/2026 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SOUZA, N. B. de; MEIRA, R. O.; PEREIRA, J. E. S. |
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Afiliação: |
NATÁLIA BARROS DE SOUZA, UNIVERSITY OF BRASÍLIA; RENNAN OLIVEIRA MEIRA; JONNY EVERSON SCHERWINSKI PEREIRA, CENARGEN. |
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Título: |
Evidence of somatic cell reprogramming toward embryogenic competence revealed through morphoanatomical and biochemical transitions in Piper aduncum L. |
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Ano de publicação: |
2026 |
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Fonte/Imprenta: |
Protoplasma, 2026. |
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DOI: |
https://doi.org/10.1007/s00709-026-02221-x |
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Idioma: |
Inglês |
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Notas: |
Na publicação: Jonny Everson Scherwinski-Pereira. |
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Conteúdo: |
Somatic embryogenesis represents a well-established example of cellular plasticity in plants, allowing differentiated somatic cells to revert to a totipotent state and regenerate whole plants. In Piper aduncum L., a species of high economic value due to its dillapiole-rich essential oil, large-scale propagation remains limited by inefficient regeneration systems. This study provides integrative evidence of somatic cell reprogramming toward embryogenic competence from adult leaf explants cultured under controlled in vitro conditions. Morphoanatomical analyses revealed that reprogramming began within three days of culture, characterized by hypertrophic nuclei, dense cytoplasm, and perivascular clusters of small isodiametric cells acting as embryogenic niches. Sequential histological and histochemical changes accompanied the transition from callus proliferation to organized somatic embryos, culminating in cotyledonary structures exhibiting all primary meristems. The growth curve of embryogenic calli revealed five physiological phases, with the linear phase (28–49 days) corresponding to the optimal window for subculture and somatic embryo differentiation. Biochemical profiling indicated dynamic redox modulation, marked by transient peaks in antioxidant enzyme activity (SOD, CAT, APX, POD) and controlled lipid peroxidation, suggesting that oxidative signaling functions as a permissive factor during embryogenic induction and differentiation. Together, these findings demonstrate that somatic embryogenesis in P. aduncum arises from a coordinated sequence of cellular and physiological reprogramming events in adult tissues, providing new insights into the developmental plasticity and redox regulation underlying plant regeneration. MenosSomatic embryogenesis represents a well-established example of cellular plasticity in plants, allowing differentiated somatic cells to revert to a totipotent state and regenerate whole plants. In Piper aduncum L., a species of high economic value due to its dillapiole-rich essential oil, large-scale propagation remains limited by inefficient regeneration systems. This study provides integrative evidence of somatic cell reprogramming toward embryogenic competence from adult leaf explants cultured under controlled in vitro conditions. Morphoanatomical analyses revealed that reprogramming began within three days of culture, characterized by hypertrophic nuclei, dense cytoplasm, and perivascular clusters of small isodiametric cells acting as embryogenic niches. Sequential histological and histochemical changes accompanied the transition from callus proliferation to organized somatic embryos, culminating in cotyledonary structures exhibiting all primary meristems. The growth curve of embryogenic calli revealed five physiological phases, with the linear phase (28–49 days) corresponding to the optimal window for subculture and somatic embryo differentiation. Biochemical profiling indicated dynamic redox modulation, marked by transient peaks in antioxidant enzyme activity (SOD, CAT, APX, POD) and controlled lipid peroxidation, suggesting that oxidative signaling functions as a permissive factor during embryogenic induction and differentiation. Together, these findings demonstrate th... Mostrar Tudo |
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Palavras-Chave: |
Clonal propagation; Dillapiole; Embryogenic competence; Redox metabolism. |
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Thesagro: |
Piperaceae. |
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Thesaurus Nal: |
Somatic embryogenesis. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02563naa a2200241 a 4500
001 2187379
005 2026-06-08
008 2026 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s00709-026-02221-x$2DOI
100 1 $aSOUZA, N. B. de
245 $aEvidence of somatic cell reprogramming toward embryogenic competence revealed through morphoanatomical and biochemical transitions in Piper aduncum L.$h[electronic resource]
260 $c2026
500 $aNa publicação: Jonny Everson Scherwinski-Pereira.
520 $aSomatic embryogenesis represents a well-established example of cellular plasticity in plants, allowing differentiated somatic cells to revert to a totipotent state and regenerate whole plants. In Piper aduncum L., a species of high economic value due to its dillapiole-rich essential oil, large-scale propagation remains limited by inefficient regeneration systems. This study provides integrative evidence of somatic cell reprogramming toward embryogenic competence from adult leaf explants cultured under controlled in vitro conditions. Morphoanatomical analyses revealed that reprogramming began within three days of culture, characterized by hypertrophic nuclei, dense cytoplasm, and perivascular clusters of small isodiametric cells acting as embryogenic niches. Sequential histological and histochemical changes accompanied the transition from callus proliferation to organized somatic embryos, culminating in cotyledonary structures exhibiting all primary meristems. The growth curve of embryogenic calli revealed five physiological phases, with the linear phase (28–49 days) corresponding to the optimal window for subculture and somatic embryo differentiation. Biochemical profiling indicated dynamic redox modulation, marked by transient peaks in antioxidant enzyme activity (SOD, CAT, APX, POD) and controlled lipid peroxidation, suggesting that oxidative signaling functions as a permissive factor during embryogenic induction and differentiation. Together, these findings demonstrate that somatic embryogenesis in P. aduncum arises from a coordinated sequence of cellular and physiological reprogramming events in adult tissues, providing new insights into the developmental plasticity and redox regulation underlying plant regeneration.
650 $aSomatic embryogenesis
650 $aPiperaceae
653 $aClonal propagation
653 $aDillapiole
653 $aEmbryogenic competence
653 $aRedox metabolism
700 1 $aMEIRA, R. O.
700 1 $aPEREIRA, J. E. S.
773 $tProtoplasma, 2026.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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