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Registro Completo |
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
09/12/2025 |
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Data da última atualização: |
09/12/2025 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OLIVEIRA, K. M. G. de; MOURA, R. B. P.; TAVEIRA, S. F.; ZIELONKA, H. L.; BRUM, D. de O.; SILVA, E. Z. M.; TRINDADE, E. da S.; CESTARI, M. M.; CADEMARTORI, P. H. G. de; MAGALHAES, W. L. E.; LEME, D. M. |
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Afiliação: |
KETELEN MICHELE GUILHERME DE OLIVEIRA, UNIVERSIDADE FEDERAL DO PARANÁ; RAYSSA BARBARY PEDROZA MOURA, UNIVERSIDADE FEDERAL DE GOIÁS; STEPHÂNIA FLEURY TAVEIRA, UNIVERSIDADE FEDERAL DE GOIÁS; HELENA LOURENÇO ZIELONKA, UNIVERSIDADE FEDERAL DO PARANÁ; DARWIN DE OLIVEIRA BRUM, UNIVERSIDADE FEDERAL DO PARANÁ; ENZO ZINI MOREIRA SILVA, UNIVERSIDADE FEDERAL DO PARANÁ; EDVALDO DA SILVA TRINDADE, UNIVERSIDADE FEDERAL DO PARANÁ; MARTA MARGARETE CESTARI, UNIVERSIDADE FEDERAL DO PARANÁ; PEDRO HENRIQUE GONZALEZ DE CADEMARTORI, UNIVERSIDADE FEDERAL DO PARANÁ; WASHINGTON LUIZ ESTEVES MAGALHAES, CNPF; DANIELA MORAIS LEME, UNIVERSIDADE FEDERAL DO PARANÁ. |
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Título: |
Exploring the applicability of the RTgill-W1 and ZFL cell line assays in aquatic hazard assessment of mineral and organic nanomaterials. |
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Ano de publicação: |
2025 |
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Fonte/Imprenta: |
Chemosphere, v. 386, p. 144617, 2025. |
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ISSN: |
0045-6535 |
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DOI: |
https://doi.org/10.1016/j.chemosphere.2025.144617 |
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Idioma: |
Inglês |
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Conteúdo: |
Many fish have been used annually to evaluate chemical hazards to aquatic environments; thus, cell-based methods, such as the RTgill-W1 and ZFL cell line assays, have been proposed as alternatives to provide information on acute toxicity to fish, mostly in screening approaches and as part of a weight of evidence (WoE) approach. These methods have been developed using soluble chemicals, which can pose challenges for testing novel types of chemicals, such as nanomaterials. Cellulose nanofiber (CNF) kraft-bleached pulp, chitosan nanoparticles (CS-NP), and silica nanoparticles (SiO2-NP) were evaluated regarding their toxicity to fish cells using the RTgill-W1 and ZFL cell line assays (in vitro). Experiments were performed using the original protocols of each assay and modified versions to enhance the bioavailability of nanoparticles (NPs) during in vitro testing. NPs characterization in the exposure media (mean diameter, polydispersity index) was also performed. Both cell lines were exposed to CNF (39–1250 mg/L), CS-NP (4.88 mg/L to 156.25 mg/L), and SiO2-NP (7.81 mg/L to 250 mg/L) for 24 h under static culture (original protocols) and orbital shaking at 150 rpm (modified protocols). Fish cell acute toxicity was determined based on cell viability readouts. There was no effect on cell viability for CNF in both cell lines, while SiO2-NP and CS-NP presented toxicity. The toxic effect levels of SiO2-NP and CS-NP were higher in the static culture than in the orbital shaking. For SiO2-NP, ZFL cells showed higher sensitivity to SiO2-NP toxicity than RTgill-W1; however, the in vitro data did not correlate with the available acute fish toxicity data (in vivo), and if these data were used for classification purposes under the United Nations' Globally Harmonized System of Classification and Labelling of Chemicals (UN GHS), this could lead to overprotective measurements. The NP characterization data demonstrated that all tested NPs exhibit higher aggregation tendencies, which helps explain the observed biological effects. Overall, these results underscore the need for improved in vitro methods to assess the toxicity of NPs to fish cells as well as provide insights into the toxicity of NPs to fish cells. MenosMany fish have been used annually to evaluate chemical hazards to aquatic environments; thus, cell-based methods, such as the RTgill-W1 and ZFL cell line assays, have been proposed as alternatives to provide information on acute toxicity to fish, mostly in screening approaches and as part of a weight of evidence (WoE) approach. These methods have been developed using soluble chemicals, which can pose challenges for testing novel types of chemicals, such as nanomaterials. Cellulose nanofiber (CNF) kraft-bleached pulp, chitosan nanoparticles (CS-NP), and silica nanoparticles (SiO2-NP) were evaluated regarding their toxicity to fish cells using the RTgill-W1 and ZFL cell line assays (in vitro). Experiments were performed using the original protocols of each assay and modified versions to enhance the bioavailability of nanoparticles (NPs) during in vitro testing. NPs characterization in the exposure media (mean diameter, polydispersity index) was also performed. Both cell lines were exposed to CNF (39–1250 mg/L), CS-NP (4.88 mg/L to 156.25 mg/L), and SiO2-NP (7.81 mg/L to 250 mg/L) for 24 h under static culture (original protocols) and orbital shaking at 150 rpm (modified protocols). Fish cell acute toxicity was determined based on cell viability readouts. There was no effect on cell viability for CNF in both cell lines, while SiO2-NP and CS-NP presented toxicity. The toxic effect levels of SiO2-NP and CS-NP were higher in the static culture than in the orbital shaking. For SiO2... Mostrar Tudo |
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Palavras-Chave: |
Material inorgânico; Material orgânico; Nanotecnologia; Risco químico. |
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Thesagro: |
Peixe; Toxidez. |
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Thesaurus Nal: |
Chemical hazards; Fish; Toxicity testing. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 03349naa a2200373 a 4500
001 2182580
005 2025-12-09
008 2025 bl uuuu u00u1 u #d
022 $a0045-6535
024 7 $ahttps://doi.org/10.1016/j.chemosphere.2025.144617$2DOI
100 1 $aOLIVEIRA, K. M. G. de
245 $aExploring the applicability of the RTgill-W1 and ZFL cell line assays in aquatic hazard assessment of mineral and organic nanomaterials.$h[electronic resource]
260 $c2025
520 $aMany fish have been used annually to evaluate chemical hazards to aquatic environments; thus, cell-based methods, such as the RTgill-W1 and ZFL cell line assays, have been proposed as alternatives to provide information on acute toxicity to fish, mostly in screening approaches and as part of a weight of evidence (WoE) approach. These methods have been developed using soluble chemicals, which can pose challenges for testing novel types of chemicals, such as nanomaterials. Cellulose nanofiber (CNF) kraft-bleached pulp, chitosan nanoparticles (CS-NP), and silica nanoparticles (SiO2-NP) were evaluated regarding their toxicity to fish cells using the RTgill-W1 and ZFL cell line assays (in vitro). Experiments were performed using the original protocols of each assay and modified versions to enhance the bioavailability of nanoparticles (NPs) during in vitro testing. NPs characterization in the exposure media (mean diameter, polydispersity index) was also performed. Both cell lines were exposed to CNF (39–1250 mg/L), CS-NP (4.88 mg/L to 156.25 mg/L), and SiO2-NP (7.81 mg/L to 250 mg/L) for 24 h under static culture (original protocols) and orbital shaking at 150 rpm (modified protocols). Fish cell acute toxicity was determined based on cell viability readouts. There was no effect on cell viability for CNF in both cell lines, while SiO2-NP and CS-NP presented toxicity. The toxic effect levels of SiO2-NP and CS-NP were higher in the static culture than in the orbital shaking. For SiO2-NP, ZFL cells showed higher sensitivity to SiO2-NP toxicity than RTgill-W1; however, the in vitro data did not correlate with the available acute fish toxicity data (in vivo), and if these data were used for classification purposes under the United Nations' Globally Harmonized System of Classification and Labelling of Chemicals (UN GHS), this could lead to overprotective measurements. The NP characterization data demonstrated that all tested NPs exhibit higher aggregation tendencies, which helps explain the observed biological effects. Overall, these results underscore the need for improved in vitro methods to assess the toxicity of NPs to fish cells as well as provide insights into the toxicity of NPs to fish cells.
650 $aChemical hazards
650 $aFish
650 $aToxicity testing
650 $aPeixe
650 $aToxidez
653 $aMaterial inorgânico
653 $aMaterial orgânico
653 $aNanotecnologia
653 $aRisco químico
700 1 $aMOURA, R. B. P.
700 1 $aTAVEIRA, S. F.
700 1 $aZIELONKA, H. L.
700 1 $aBRUM, D. de O.
700 1 $aSILVA, E. Z. M.
700 1 $aTRINDADE, E. da S.
700 1 $aCESTARI, M. M.
700 1 $aCADEMARTORI, P. H. G. de
700 1 $aMAGALHAES, W. L. E.
700 1 $aLEME, D. M.
773 $tChemosphere$gv. 386, p. 144617, 2025.
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| 2. |  | ZANUNCIO, J. C.; MOLINA-RUGAMA, A.J.; SANTOS, G. P.; RAMALHO, F. de S. Effect of body weight on fecundity and longevity of the stinkbug predator Podisus rostralis. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 37, n. 9, p. 1225-1230, set. 2002 Título em português: Efeito do peso corporal na fecundidade e na longevidade do percevejo predador Podisus rostralis.| Biblioteca(s): Embrapa Unidades Centrais. |
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