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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
25/11/2025 |
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Data da última atualização: |
25/11/2025 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
FONSECA, P. L. C.; SANTOS, M. S.; SILVA, J. DOS S.; ARMACHE, J. N.; NASCIMENTO, R. S.; SANTOS, J. P. N.; PIRES, G. R. R.; FARIA, I. J. DA S. DE; OCAMPOS, Y. M.; ASTUA, J. de F.; RAMOS-GONZALÉZ, P. L.; SANTANA, J. O.; FERNANDES, V. F.; MARQUES, J. P. R.; TASSI, A. D.; SODRÉ, G. A.; LUIZ, L. C. G. C.; LUIZ, W. B.; PIROVANI, C. P.; OLIVEIRA, A. R.; AGUIAR, E. R. G. R. |
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Afiliação: |
PAULA LUIZE CAMARGOS FONSECA, UNIVERSIDADE FEDERAL DE MINAS GERAIS; MIRIAN S. SANTOS, UNIVERSIDADE ESTADUAL DE SANTA CRUZ; JONATHA DOS SANTOS SILVA, UNIVERSIDADE ESTADUAL DE SANTA CRUZ; JULIANA N. ARMACHE, UNIVERSIDADE FEDERAL DE MINAS GERAIS; RENATA S. NASCIMENTO, UNIVERSIDADE ESTADUAL DE SANTA CRUZ; JOÃO PEDRO NUNES SANTOS, UNIVERSIDADE ESTADUAL DE SANTA CRUZ; GLEICE R. R. PIRES, UNIVERSIDADE DE SÃO PAULO; ISAQUE JOÃO DA SILVA DE FARIA, UNIVERSIDADE FEDERAL DE MINAS GERAIS; YULIANA MORA OCAMPOS, UNIVERSIDADE ESTADUAL DE SANTA CRUZ; JULIANA DE FREITAS ASTUA, CNPMF; PEDRO L. RAMOS-GONZALÉZ, INSTITUTO BIOLÓGICO DE SÃO PAULO; JULIANO O. SANTANA, UNIVERSIDADE ESTADUAL DE SANTA CRUZ; VALÉRIA F. FERNANDES, UNIVERSIDADE ESTADUAL DE SANTA CRUZ; JOÃO P. R. MARQUES, UNIVERSIDADE DE SÃO PAULO; ALINE D. TASSI; GEORGE A. SODRÉ, UNIVERSIDADE ESTADUAL DE SANTA CRUZ; LUCIENE CRISTINA GASTALHO CAMPOS LUIZ, UNIVERSIDADE ESTADUAL DE SANTA CRUZ; WILSON B. LUIZ, UNIVERSIDADE ESTADUAL DE SANTA CRUZ; CARLOS P. PIROVANI, UNIVERSIDADE ESTADUAL DE SANTA CRUZ; ANIBAL R. OLIVEIRA, UNIVERSIDADE ESTADUAL DE SANTA CRUZ; ERIC R. G. R. AGUIAR, UNIVERSIDADE ESTADUAL DE SANTA CRUZ. |
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Título: |
Integrative multi-omics approach identified emerging viral threat in Theobroma cacao plants. |
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Ano de publicação: |
2025 |
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Fonte/Imprenta: |
BMC Plant Biology, n.25, v. 1425, 2025. |
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ISSN: |
1471-2229 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract Background Theobroma cacao seeds are the source of chocolate and other valuable products. Pathogens can damage cocoa beans, reducing yield and quality. This study examined viruses linked to chlorosis and leaf deformation in cocoa plants in Bahia, Brazil, using an integrative approach. Results Macroscopic and microscopic analysis of symptomatic T. cacao leaves revealed chlorosis and histological alterations consistent with viral infection, including cell and nuclear hypertrophy. Deep sequencing of small RNA (sRNA) from these tissues identified virus-derived sRNA (vsiRNAs) profile, which suggests that viral transcripts activate the antiviral RNA interference pathways. The vsiRNAs were used to assemble two viral sequences: one related to geminivirids and the other resembling a strain of the recently described Cacao swollen shoot Ghana S virus, a putative member of the family Caulimoviridae commonly found as an Endogenous Viral Element (EVE) in symptomless plants. The geminivirid was tentatively designated Citlodavirus theobromae, while the badnavirus isolate was named Brazilian cacao swollen shoot Ghana S virus Ilhéus. Mass spectrometry detected multiple viral peptides from coding genes from both viral genomes, supporting the potential occurrence of viral translation and replication. Notably, symptoms were observed only in association with the geminivirids. Although viral DNA from both viruses was present in symptomatic and asymptomatic leaves of the same plant, geminivirid RNA was exclusively detected in symptomatic tissues, suggesting a correlation between the citlodavirustranscript accumulation and symptom expression. Conclusion Our findings highlight the emergence of a new viral geminivirid threat that could affect cocoa production in South America. MenosAbstract Background Theobroma cacao seeds are the source of chocolate and other valuable products. Pathogens can damage cocoa beans, reducing yield and quality. This study examined viruses linked to chlorosis and leaf deformation in cocoa plants in Bahia, Brazil, using an integrative approach. Results Macroscopic and microscopic analysis of symptomatic T. cacao leaves revealed chlorosis and histological alterations consistent with viral infection, including cell and nuclear hypertrophy. Deep sequencing of small RNA (sRNA) from these tissues identified virus-derived sRNA (vsiRNAs) profile, which suggests that viral transcripts activate the antiviral RNA interference pathways. The vsiRNAs were used to assemble two viral sequences: one related to geminivirids and the other resembling a strain of the recently described Cacao swollen shoot Ghana S virus, a putative member of the family Caulimoviridae commonly found as an Endogenous Viral Element (EVE) in symptomless plants. The geminivirid was tentatively designated Citlodavirus theobromae, while the badnavirus isolate was named Brazilian cacao swollen shoot Ghana S virus Ilhéus. Mass spectrometry detected multiple viral peptides from coding genes from both viral genomes, supporting the potential occurrence of viral translation and replication. Notably, symptoms were observed only in association with the geminivirids. Although viral DNA from both viruses was present in symptomatic and asymptomatic leaves of the same plant, gemini... Mostrar Tudo |
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Thesagro: |
Vírus. |
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Categoria do assunto: |
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Marc: |
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001 2181912
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022 $a1471-2229
100 1 $aFONSECA, P. L. C.
245 $aIntegrative multi-omics approach identified emerging viral threat in Theobroma cacao plants.$h[electronic resource]
260 $c2025
520 $aAbstract Background Theobroma cacao seeds are the source of chocolate and other valuable products. Pathogens can damage cocoa beans, reducing yield and quality. This study examined viruses linked to chlorosis and leaf deformation in cocoa plants in Bahia, Brazil, using an integrative approach. Results Macroscopic and microscopic analysis of symptomatic T. cacao leaves revealed chlorosis and histological alterations consistent with viral infection, including cell and nuclear hypertrophy. Deep sequencing of small RNA (sRNA) from these tissues identified virus-derived sRNA (vsiRNAs) profile, which suggests that viral transcripts activate the antiviral RNA interference pathways. The vsiRNAs were used to assemble two viral sequences: one related to geminivirids and the other resembling a strain of the recently described Cacao swollen shoot Ghana S virus, a putative member of the family Caulimoviridae commonly found as an Endogenous Viral Element (EVE) in symptomless plants. The geminivirid was tentatively designated Citlodavirus theobromae, while the badnavirus isolate was named Brazilian cacao swollen shoot Ghana S virus Ilhéus. Mass spectrometry detected multiple viral peptides from coding genes from both viral genomes, supporting the potential occurrence of viral translation and replication. Notably, symptoms were observed only in association with the geminivirids. Although viral DNA from both viruses was present in symptomatic and asymptomatic leaves of the same plant, geminivirid RNA was exclusively detected in symptomatic tissues, suggesting a correlation between the citlodavirustranscript accumulation and symptom expression. Conclusion Our findings highlight the emergence of a new viral geminivirid threat that could affect cocoa production in South America.
650 $aVírus
700 1 $aSANTOS, M. S.
700 1 $aSILVA, J. DOS S.
700 1 $aARMACHE, J. N.
700 1 $aNASCIMENTO, R. S.
700 1 $aSANTOS, J. P. N.
700 1 $aPIRES, G. R. R.
700 1 $aFARIA, I. J. DA S. DE
700 1 $aOCAMPOS, Y. M.
700 1 $aASTUA, J. de F.
700 1 $aRAMOS-GONZALÉZ, P. L.
700 1 $aSANTANA, J. O.
700 1 $aFERNANDES, V. F.
700 1 $aMARQUES, J. P. R.
700 1 $aTASSI, A. D.
700 1 $aSODRÉ, G. A.
700 1 $aLUIZ, L. C. G. C.
700 1 $aLUIZ, W. B.
700 1 $aPIROVANI, C. P.
700 1 $aOLIVEIRA, A. R.
700 1 $aAGUIAR, E. R. G. R.
773 $tBMC Plant Biology$gn.25
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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