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Registro Completo |
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
11/11/2025 |
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Data da última atualização: |
17/11/2025 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BETTENCOURT, G. M. de F.; DEGENHARDT, J.; SANTOS, G. D. dos; VICENTE, V. A.; SOCCOL, C. R. |
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Afiliação: |
GISELA MANUELA DE FRANÇA BETTENCOURT, UNIVERSIDADE FEDERAL DO PARANA; JULIANA DEGENHARDT GOLDBACH, CNPF; GERMANA DAVILA DOS SANTOS, UNIVERSIDADE FEDERAL DO PARANA; VÂNIA APARECIDA VICENTE, UNIVERSIDADE FEDERAL DO PARANA; CARLOS RICARDO SOCCOL, UNIVERSIDADE FEDERAL DO PARANA. |
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Título: |
Metagenomic analyses, isolation and characterization of endophytic bacteria associated with Eucalyptus urophylla BRS07-01 in vitro plants. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
World Journal of Microbiology and Biotechnology, v. 37, n. 164, p. 1-16, 2021. |
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ISSN: |
0959-3993 |
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DOI: |
10.1007/s11274-021-03127-x |
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Idioma: |
Inglês |
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Conteúdo: |
Eucalyptus is the main species for the forestry industry in Brazil. Biotechnology and, more recently, gene editing offer significant opportunities for rapid improvements in Eucalyptus breeding programs. However, the recalcitrance of Eucalyptus species to in vitro culture is also a major limitation for commercial deployment of biotechnology techniques in Eucalyptus improvement. We evaluated various clones of Eucalyptus urophylla for their in vitro regeneration potential identified a clone, BRS07-01, with considerably higher regeneration rate (85%) in organogenesis, and significantly higher than most works described in literature. Endophytic bacteria are widely reported to improve in vitro plant growth and development. Hence, we believe that inclusion of endophytic plant growth promoting bacteria enhanced was responsible for the improved plantlets growth and development of this clone under in vitro culture. Metagenomic analysis was performed to isolate and characterize the prominent endophytic bacteria on BRS07-01 leaf tissue in vitro micro-cultures, and evaluate their impact on plant growth promotion. The analysis revealed the presence of the phyla Firmicutes (35%), Proteobacteria (30%) and much smaller quantities of Actinobacteria, Bacteroidetes, Gemmatimonadetes, Crenarchaeota, Euryarchaeota and Acidobacteria. Of the thirty endophytic bacterial strains isolated, eleven produced indole-3-acetic acid. Two of the isolates were identified as Enterobacter sp. and Paenibacillus polymyxa, which are nitrogen-fixing and capable of phosphate and produce ammonium. These isolates also showed similar positive effects on the germination of common beans (Phaseolus spp.). The isolates will now be tested as a growth promoter in Eucalyptus in vitro cultures. Graphical abstract for the methodology using cultivation independent and dependent methodologies to investigate the endophytic bacteria community from in vitro Eucalyptus urophylla BRS07-01. MenosEucalyptus is the main species for the forestry industry in Brazil. Biotechnology and, more recently, gene editing offer significant opportunities for rapid improvements in Eucalyptus breeding programs. However, the recalcitrance of Eucalyptus species to in vitro culture is also a major limitation for commercial deployment of biotechnology techniques in Eucalyptus improvement. We evaluated various clones of Eucalyptus urophylla for their in vitro regeneration potential identified a clone, BRS07-01, with considerably higher regeneration rate (85%) in organogenesis, and significantly higher than most works described in literature. Endophytic bacteria are widely reported to improve in vitro plant growth and development. Hence, we believe that inclusion of endophytic plant growth promoting bacteria enhanced was responsible for the improved plantlets growth and development of this clone under in vitro culture. Metagenomic analysis was performed to isolate and characterize the prominent endophytic bacteria on BRS07-01 leaf tissue in vitro micro-cultures, and evaluate their impact on plant growth promotion. The analysis revealed the presence of the phyla Firmicutes (35%), Proteobacteria (30%) and much smaller quantities of Actinobacteria, Bacteroidetes, Gemmatimonadetes, Crenarchaeota, Euryarchaeota and Acidobacteria. Of the thirty endophytic bacterial strains isolated, eleven produced indole-3-acetic acid. Two of the isolates were identified as Enterobacter sp. and Paenibacillus p... Mostrar Tudo |
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Palavras-Chave: |
Bactéria endofítica; Bioinoculant; Bioinoculante; Cultivar BRS07-01; IAA production. |
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Thesagro: |
Cultura In Vitro; Estimulante de Crescimento Vegetal; Eucalyptus Urophylla; Inoculante. |
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Thesaurus Nal: |
Bacillus endophyticus; Paenibacillus polymyxa; Plant growth. |
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Categoria do assunto: |
K Ciência Florestal e Produtos de Origem Vegetal |
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Marc: |
LEADER 03052naa a2200337 a 4500
001 2181385
005 2025-11-17
008 2021 bl uuuu u00u1 u #d
022 $a0959-3993
024 7 $a10.1007/s11274-021-03127-x$2DOI
100 1 $aBETTENCOURT, G. M. de F.
245 $aMetagenomic analyses, isolation and characterization of endophytic bacteria associated with Eucalyptus urophylla BRS07-01 in vitro plants.$h[electronic resource]
260 $c2021
520 $aEucalyptus is the main species for the forestry industry in Brazil. Biotechnology and, more recently, gene editing offer significant opportunities for rapid improvements in Eucalyptus breeding programs. However, the recalcitrance of Eucalyptus species to in vitro culture is also a major limitation for commercial deployment of biotechnology techniques in Eucalyptus improvement. We evaluated various clones of Eucalyptus urophylla for their in vitro regeneration potential identified a clone, BRS07-01, with considerably higher regeneration rate (85%) in organogenesis, and significantly higher than most works described in literature. Endophytic bacteria are widely reported to improve in vitro plant growth and development. Hence, we believe that inclusion of endophytic plant growth promoting bacteria enhanced was responsible for the improved plantlets growth and development of this clone under in vitro culture. Metagenomic analysis was performed to isolate and characterize the prominent endophytic bacteria on BRS07-01 leaf tissue in vitro micro-cultures, and evaluate their impact on plant growth promotion. The analysis revealed the presence of the phyla Firmicutes (35%), Proteobacteria (30%) and much smaller quantities of Actinobacteria, Bacteroidetes, Gemmatimonadetes, Crenarchaeota, Euryarchaeota and Acidobacteria. Of the thirty endophytic bacterial strains isolated, eleven produced indole-3-acetic acid. Two of the isolates were identified as Enterobacter sp. and Paenibacillus polymyxa, which are nitrogen-fixing and capable of phosphate and produce ammonium. These isolates also showed similar positive effects on the germination of common beans (Phaseolus spp.). The isolates will now be tested as a growth promoter in Eucalyptus in vitro cultures. Graphical abstract for the methodology using cultivation independent and dependent methodologies to investigate the endophytic bacteria community from in vitro Eucalyptus urophylla BRS07-01.
650 $aBacillus endophyticus
650 $aPaenibacillus polymyxa
650 $aPlant growth
650 $aCultura In Vitro
650 $aEstimulante de Crescimento Vegetal
650 $aEucalyptus Urophylla
650 $aInoculante
653 $aBactéria endofítica
653 $aBioinoculant
653 $aBioinoculante
653 $aCultivar BRS07-01
653 $aIAA production
700 1 $aDEGENHARDT, J.
700 1 $aSANTOS, G. D. dos
700 1 $aVICENTE, V. A.
700 1 $aSOCCOL, C. R.
773 $tWorld Journal of Microbiology and Biotechnology$gv. 37, n. 164, p. 1-16, 2021.
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| 1. |  | GUIMARÃES, L. A.; DUSI, D. M. A.; SILVEIRA, E. D.; DORNELAS, M. D.; CARNEIRO, V. T. C. Expression and phylogenetic analysis of two putative MADS-box like genes of Brachiaria brizantha (A. Rich.) Stapf. In: CONGRESSO INTERNACIONAL DE REPRODUÇÃO SEXUAL EM PLANTAS, 20., 2008, Brasília, DF. [Anais...]. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2008. 240 p. (Embrapa Recursos Genéticos e Biotecnologia. Documentos, 259). p. 202. Apresentação oral.| Tipo: Resumo em Anais de Congresso |
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