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 | Acesso ao texto completo restrito à biblioteca da Embrapa Recursos Genéticos e Biotecnologia. Para informações adicionais entre em contato com cenargen.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
17/09/2025 |
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Data da última atualização: |
17/09/2025 |
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Autoria: |
FARIA, O. A. C.; KUSSANO, N. R.; MOURA, A. O.; FARIA, L. C.; MARTINS, L. P.; SPRÍCIGO, J. F. W.; FRANCO, M. M.; DODE, M. A. N. |
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Afiliação: |
O. A. C. FARIA, UNIVERSITY OF BRASILIA; A. O. MOURA, FEDERAL UNIVERSITY OF UBERLÂNDIA; L. C. FARIA, UNIVERSITY OF BRASILIA; L. P. MARTINS, UNIVERSITY OF BRASILIA; J. F. W. SPRÍCIGO, FEDERAL UNIVERSITY OF GOIÁS; MAURICIO MACHAIM FRANCO, CENARGEN; MARGOT ALVES NUNES DODE, CENARGEN. |
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Título: |
Physiological oocyte maturation via intrafollicular transfer improves metabolic and molecular quality of bovine embryos. |
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Ano de publicação: |
2026 |
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Fonte/Imprenta: |
Theriogenology, v. 249, 117642, 2026. |
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DOI: |
https://doi.org/10.1016/j.theriogenology.2025.117642 |
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Idioma: |
Inglês |
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Conteúdo: |
In vitro maturation (IVM) has been widely used in bovine embryo production but is often associated with increased lipid accumulation and reduced cryotolerance. This study evaluated the intrafollicular transfer of immature oocytes (IFIOT) as an alternative oocyte maturation strategy, comparing it with conventional IVM in terms of embryo development, lipid metabolism, gene expression, cryosurvival, and DNA methylation. After maturation, oocytes were fertilized and cultured in vitro. Embryos were assessed for cleavage and blastocyst rates, lipid and mitochondrial content, total cell number, and survival after cryopreservation. Expanded blastocysts at Day 7 were transferred to synchronized recipients, and Day 14 embryos were collected for gene expression and methylation analyses. Cleavage and blastocyst rates were similar between groups (P > 0.05), but lipid content was significantly higher in IVM embryos (P < 0.05). Expression of PPARγ, a key transcription factor regulating lipid metabolism, was significantly higher in IVM-derived embryos (P < 0.0001), suggesting a more unbalanced metabolic profile compared to IFIOT. Mitochondrial activity and cell number did not differ. Overall cryosurvival was comparable; however, Day 8 IVM embryos showed reduced post-thaw survival relative to IFIOT (P < 0.05). DNA methylation levels in Satellite I and α-Satellite regions remained low in both groups, with no significant differences. These findings indicate that IFIOT supports oocyte maturation under more physiological conditions, leading to embryos with improved metabolic balance, reduced lipid accumulation, and enhanced cryotolerance. IFIOT may represent a valuable tool to optimize oocyte quality and increase the robustness of bovine in vitro embryo production systems. MenosIn vitro maturation (IVM) has been widely used in bovine embryo production but is often associated with increased lipid accumulation and reduced cryotolerance. This study evaluated the intrafollicular transfer of immature oocytes (IFIOT) as an alternative oocyte maturation strategy, comparing it with conventional IVM in terms of embryo development, lipid metabolism, gene expression, cryosurvival, and DNA methylation. After maturation, oocytes were fertilized and cultured in vitro. Embryos were assessed for cleavage and blastocyst rates, lipid and mitochondrial content, total cell number, and survival after cryopreservation. Expanded blastocysts at Day 7 were transferred to synchronized recipients, and Day 14 embryos were collected for gene expression and methylation analyses. Cleavage and blastocyst rates were similar between groups (P > 0.05), but lipid content was significantly higher in IVM embryos (P < 0.05). Expression of PPARγ, a key transcription factor regulating lipid metabolism, was significantly higher in IVM-derived embryos (P < 0.0001), suggesting a more unbalanced metabolic profile compared to IFIOT. Mitochondrial activity and cell number did not differ. Overall cryosurvival was comparable; however, Day 8 IVM embryos showed reduced post-thaw survival relative to IFIOT (P < 0.05). DNA methylation levels in Satellite I and α-Satellite regions remained low in both groups, with no significant differences. These findings indicate that IFIOT supports oocyte maturatio... Mostrar Tudo |
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Palavras-Chave: |
D14 embryo; In vitro embryo production. |
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Thesaurus Nal: |
DNA methylation; Gene expression; Lipid metabolism. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02656naa a2200277 a 4500
001 2178880
005 2025-09-17
008 2026 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.theriogenology.2025.117642$2DOI
100 1 $aFARIA, O. A. C.
245 $aPhysiological oocyte maturation via intrafollicular transfer improves metabolic and molecular quality of bovine embryos.$h[electronic resource]
260 $c2026
520 $aIn vitro maturation (IVM) has been widely used in bovine embryo production but is often associated with increased lipid accumulation and reduced cryotolerance. This study evaluated the intrafollicular transfer of immature oocytes (IFIOT) as an alternative oocyte maturation strategy, comparing it with conventional IVM in terms of embryo development, lipid metabolism, gene expression, cryosurvival, and DNA methylation. After maturation, oocytes were fertilized and cultured in vitro. Embryos were assessed for cleavage and blastocyst rates, lipid and mitochondrial content, total cell number, and survival after cryopreservation. Expanded blastocysts at Day 7 were transferred to synchronized recipients, and Day 14 embryos were collected for gene expression and methylation analyses. Cleavage and blastocyst rates were similar between groups (P > 0.05), but lipid content was significantly higher in IVM embryos (P < 0.05). Expression of PPARγ, a key transcription factor regulating lipid metabolism, was significantly higher in IVM-derived embryos (P < 0.0001), suggesting a more unbalanced metabolic profile compared to IFIOT. Mitochondrial activity and cell number did not differ. Overall cryosurvival was comparable; however, Day 8 IVM embryos showed reduced post-thaw survival relative to IFIOT (P < 0.05). DNA methylation levels in Satellite I and α-Satellite regions remained low in both groups, with no significant differences. These findings indicate that IFIOT supports oocyte maturation under more physiological conditions, leading to embryos with improved metabolic balance, reduced lipid accumulation, and enhanced cryotolerance. IFIOT may represent a valuable tool to optimize oocyte quality and increase the robustness of bovine in vitro embryo production systems.
650 $aDNA methylation
650 $aGene expression
650 $aLipid metabolism
653 $aD14 embryo
653 $aIn vitro embryo production
700 1 $aKUSSANO, N. R.
700 1 $aMOURA, A. O.
700 1 $aFARIA, L. C.
700 1 $aMARTINS, L. P.
700 1 $aSPRÍCIGO, J. F. W.
700 1 $aFRANCO, M. M.
700 1 $aDODE, M. A. N.
773 $tTheriogenology$gv. 249, 117642, 2026.
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| 1. |  | SÁ, M. K. N. de; CAMPOS, F. S.; GOIS, G. C.; ARAÚJO, C. de A.; GUIMARÃESM N. J. M.; MAGALHÃES, A. L. R.; SIMOES, W. L.; ARAUJO, G. G. L. de. Composição mineral em silagens de sorgo cultivar BRS Ponta negra irrigado com diferentes frações de lixiviação. In: SIMPÓSIO PARAIBANO DE CONSERVAÇÃO E UTILIZAÇÃO DE FORRAGENS: TECNOLOGIAS E INOVAÇÕES PARA A PECUÁRIA NORDESTINA, 1., 2019, Areia. Anais... Areia: Universidade Federal da Paraíba, 2019.| Tipo: Artigo em Anais de Congresso |
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