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Registro Completo |
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Biblioteca(s): |
Embrapa Instrumentação. |
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Data corrente: |
08/09/2025 |
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Data da última atualização: |
22/09/2025 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
DOWNEY, K.; RONDA, K.; COSTA, P. M.; PELLIZZARI, J.; LYSAK, D. H.; WOLF, W. W.; STEINER, K.; ELLIOTT, C.; HABER, A.; BUSSE, V.; BUSSE, F.; GOERLING, B.; SUSZCZYNSKI, C.; BOEHMER, S.; KOCK, F. V.; MORAES, T. B.; COLNAGO, L. A.; SIMPSON, M. J.; SIMPSON, A. J. |
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Afiliação: |
ENVIRONMENTAL NMR CENTRE, UNIVERSITY OF TORONTO SCARBOROUGH; ENVIRONMENTAL NMR CENTRE, UNIVERSITY OF TORONTO SCARBOROUGH; UNIVERSITY OF TORONTO SCARBOROUGH, TORONTO; UNIVERSITY OF TORONTO SCARBOROUGH, TORONTO; UNIVERSITY OF TORONTO SCARBOROUGH, TORONTO; UNIVERSITY OF TORONTO SCARBOROUGH, TORONTO; UNIVERSITY OF TORONTO SCARBOROUGH, TORONTO; BRUKER BIOSPIN GMBH & CO., ETTLINGEN; BRUKER BIOSPIN GMBH & CO., ETTLINGEN; BRUKER SWITZERLAND AG, FÄLLANDEN 8117; BRUKER SWITZERLAND AG, FÄLLANDEN 8117; BRUKER BIOSPIN GMBH & CO., ETTLINGEN; ISOTEC STABLE ISOTOPE DIVISION, MILLIPORE SIGMA, BURLINGTON, MASSACHUSETTS 01803; ISOTEC STABLE ISOTOPE DIVISION, MILLIPORE SIGMA, BURLINGTON, MASSACHUSETTS 01803; ENVIRONMENTAL NMR CENTRE, UNIVERSITY OF TORONTO SCARBOROUGH, TORONTO; DEPARTMENT OF BIOSYSTEMS ENGINEERING, SÃO PAULO UNIVERSITY; LUIZ ALBERTO COLNAGO, CNPDIA; ENVIRONMENTAL NMR CENTRE, UNIVERSITY OF TORONTO SCARBOROUGH, TORONTO; ENVIRONMENTAL NMR CENTRE, UNIVERSITY OF TORONTO SCARBOROUGH, TORONTO. |
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Título: |
Benchtop NMR Spectroscopy of In Vivo Multicellular Organisms. |
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Ano de publicação: |
2025 |
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Fonte/Imprenta: |
Analytical Chemistry, v. 97, 2025. |
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Páginas: |
13561−13568 |
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DOI: |
https://doi.org/10.1021/acs.analchem.5c02023 |
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Idioma: |
Inglês |
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Conteúdo: |
NMR spectroscopy is a critical tool for environmental and biological research, but the physical and financial barriers of standard “high-field” NMR spectrometers can limit applications, especially in the environmental sciences. Low-field benchtop NMR (1 H resonance frequencies generally ≤100 MHz) is more accessible, but its lower sensitivity and increased spectral overlap have limited the study of complex samples. Living organisms are among the most heterogeneous samples, and it is unclear if useful information can be extracted in vivo using benchtop NMR. Here, the potential of low-field (80 MHz) in vivo NMR is first assessed by analyzing 13C-labeling of unicellular green algae and then by monitoring a process within a multicellular organism (T. californicus). This is followed by studying live brine shrimp (A. franciscana) at 13C natural abundance. Adults are compared to brine shrimp cysts, with a number of spectral assignments possible and differences between the life stages clearly evident. High-field NMR is used to confirm peak assignments and provide a more comprehensive characterization of biomolecules present, ultimately making the low-field NMR data more useful. Standard experiments such as 1D 1 H, 1D 13C and 2D HSQC are conducted, as well as more advanced experiments such as 13C-SSFP, which greatly enhances 13C sensitivity, and reverse HSQC, which decreases spectral overlap. Ultimately, this work demonstrates that low-field NMR can effectively analyze live organisms with or without isotopic enrichment and that it holds great potential for future work, such as in vivo analysis of organisms directly in the field if/when portable NMR spectrometers become available. MenosNMR spectroscopy is a critical tool for environmental and biological research, but the physical and financial barriers of standard “high-field” NMR spectrometers can limit applications, especially in the environmental sciences. Low-field benchtop NMR (1 H resonance frequencies generally ≤100 MHz) is more accessible, but its lower sensitivity and increased spectral overlap have limited the study of complex samples. Living organisms are among the most heterogeneous samples, and it is unclear if useful information can be extracted in vivo using benchtop NMR. Here, the potential of low-field (80 MHz) in vivo NMR is first assessed by analyzing 13C-labeling of unicellular green algae and then by monitoring a process within a multicellular organism (T. californicus). This is followed by studying live brine shrimp (A. franciscana) at 13C natural abundance. Adults are compared to brine shrimp cysts, with a number of spectral assignments possible and differences between the life stages clearly evident. High-field NMR is used to confirm peak assignments and provide a more comprehensive characterization of biomolecules present, ultimately making the low-field NMR data more useful. Standard experiments such as 1D 1 H, 1D 13C and 2D HSQC are conducted, as well as more advanced experiments such as 13C-SSFP, which greatly enhances 13C sensitivity, and reverse HSQC, which decreases spectral overlap. Ultimately, this work demonstrates that low-field NMR can effectively analyze live organisms ... Mostrar Tudo |
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Palavras-Chave: |
Biochemical processes; Multicellular organisms. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02734naa a2200385 a 4500
001 2178629
005 2025-09-22
008 2025 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1021/acs.analchem.5c02023$2DOI
100 1 $aDOWNEY, K.
245 $aBenchtop NMR Spectroscopy of In Vivo Multicellular Organisms.$h[electronic resource]
260 $c2025
300 $a13561−13568
520 $aNMR spectroscopy is a critical tool for environmental and biological research, but the physical and financial barriers of standard “high-field” NMR spectrometers can limit applications, especially in the environmental sciences. Low-field benchtop NMR (1 H resonance frequencies generally ≤100 MHz) is more accessible, but its lower sensitivity and increased spectral overlap have limited the study of complex samples. Living organisms are among the most heterogeneous samples, and it is unclear if useful information can be extracted in vivo using benchtop NMR. Here, the potential of low-field (80 MHz) in vivo NMR is first assessed by analyzing 13C-labeling of unicellular green algae and then by monitoring a process within a multicellular organism (T. californicus). This is followed by studying live brine shrimp (A. franciscana) at 13C natural abundance. Adults are compared to brine shrimp cysts, with a number of spectral assignments possible and differences between the life stages clearly evident. High-field NMR is used to confirm peak assignments and provide a more comprehensive characterization of biomolecules present, ultimately making the low-field NMR data more useful. Standard experiments such as 1D 1 H, 1D 13C and 2D HSQC are conducted, as well as more advanced experiments such as 13C-SSFP, which greatly enhances 13C sensitivity, and reverse HSQC, which decreases spectral overlap. Ultimately, this work demonstrates that low-field NMR can effectively analyze live organisms with or without isotopic enrichment and that it holds great potential for future work, such as in vivo analysis of organisms directly in the field if/when portable NMR spectrometers become available.
653 $aBiochemical processes
653 $aMulticellular organisms
700 1 $aRONDA, K.
700 1 $aCOSTA, P. M.
700 1 $aPELLIZZARI, J.
700 1 $aLYSAK, D. H.
700 1 $aWOLF, W. W.
700 1 $aSTEINER, K.
700 1 $aELLIOTT, C.
700 1 $aHABER, A.
700 1 $aBUSSE, V.
700 1 $aBUSSE, F.
700 1 $aGOERLING, B.
700 1 $aSUSZCZYNSKI, C.
700 1 $aBOEHMER, S.
700 1 $aKOCK, F. V.
700 1 $aMORAES, T. B.
700 1 $aCOLNAGO, L. A.
700 1 $aSIMPSON, M. J.
700 1 $aSIMPSON, A. J.
773 $tAnalytical Chemistry$gv. 97, 2025.
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