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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
11/07/2025 |
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Data da última atualização: |
14/07/2025 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OLIVEIRA, M. S. de; SILVA, N. P. da; PAULA, M. S. de; PORTO, D. L.; ARAGAO, C. F. S.; SEGURA, M. E. C.; FABRI, R. L.; BEMQUERER, M. P.; BRANDAO, H. de M.; TAVARES, G. D.; APOLONIO, A. C. M. |
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Afiliação: |
MARIANA SIMOES DE OLIVEIRA, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; NATALIA PRADO DA SILVA, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; MARCELLE SILVA ALVES DE PAULA, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; DAYANNE LOPES PORTO, UNIVERSIDADE FEDERAL DO RIO GRANDE DO NORTE; CÍCERO FLAVIO SOARES ARAGAO, UNIVERSIDADE FEDERAL DO RIO GRANDE DO NORTE; MARIA ESPERANZA CORTES SEGURA, UNIVERSIDADE FEDERAL DE MINAS GERAIS; RODRIGO LUIZ FABRI, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; MARCELO PORTO BEMQUERER, CNPGL; HUMBERTO DE MELLO BRANDAO, CNPGL; GUILHERME DINIZ TAVARES, UNIVERSIDADE FEDERAL DE JUIZ DE FORA; ANA CAROLINA MORAIS APOLONIO, UNIVERSIDADE FEDERAL DE JUIZ DE FORA. |
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Título: |
Poly(ε-caprolactone) nanocapsules coated with Chitosan optimize the antimicrobial activity of Tea Tree Oil and Azithromycin against oral pathogens. |
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Ano de publicação: |
2025 |
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Fonte/Imprenta: |
Microbial Pathogenesis, v. 206, 107820, 2025. |
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DOI: |
https://doi.org/10.1016/j.micpath.2025.107820 |
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Idioma: |
Português |
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Conteúdo: |
This study aimed to develop poly(ε-caprolactone) nanocapsules coated with chitosan, loaded with Tea Tree Oil (TTO) and Azithromycin (AZI), to optimize the antimicrobial activity and bioavailability of these actives. The nanocapsules suspension (NP-TTO-AZI-CH) was prepared using the preformed polymer interfacial precipitation method and coated with a 2 % chitosan solution. Physicochemical characterization included evaluating the pH, mean hydrodynamic diameter (Z-Average), Zeta potential (ZP), polydispersity index (PdI), encapsulation efficiency (EE) of the essential oil, and morphological assessment by Scanning Electron Microscopy (SEM) and Atomic Force Microscopy (AFM). Biological tests conducted encompassed Minimum Inhibitory Concentration (MIC), Minimum Microbicidal Concentration (MMC), and cell viability assessments. NP-TTO-AZI-CH showed a ZAverage of 238.5 ± 1.7 nm and a PdI of 0.245 ± 0.010, indicating homogeneity in particle size and distribution. The positive ZP (+28.06 ± 1.10 mV) suggested the presence of chitosan on the nanocapsule surfaces, making the suspension more acidic (pH 4.38). EE was high at 89.89 ± 1.29 %. SEM images revealed the spherical/oval shape of the nanocapsules, and AFM confirmed their nanometric size and distribution within the system. Encapsulated TTO and AZI demonstrated lower MIC values compared to their free forms for all tested strains within 24 h, indicating enhanced antimicrobial efficacy. NP-TTO-AZI-CH exhibited bactericidal activity and maintained 70 % cell viability within the first 24 h at concentrations ranging from 0.5 mg/mL + 20 μg/mL to 0.06 mg/mL + 2.5 μg/mL (TTO + AZI). This formulation proved to be a promising tool for enhancing the antimicrobial activity of bioactives and combating various oral microbial strains. MenosThis study aimed to develop poly(ε-caprolactone) nanocapsules coated with chitosan, loaded with Tea Tree Oil (TTO) and Azithromycin (AZI), to optimize the antimicrobial activity and bioavailability of these actives. The nanocapsules suspension (NP-TTO-AZI-CH) was prepared using the preformed polymer interfacial precipitation method and coated with a 2 % chitosan solution. Physicochemical characterization included evaluating the pH, mean hydrodynamic diameter (Z-Average), Zeta potential (ZP), polydispersity index (PdI), encapsulation efficiency (EE) of the essential oil, and morphological assessment by Scanning Electron Microscopy (SEM) and Atomic Force Microscopy (AFM). Biological tests conducted encompassed Minimum Inhibitory Concentration (MIC), Minimum Microbicidal Concentration (MMC), and cell viability assessments. NP-TTO-AZI-CH showed a ZAverage of 238.5 ± 1.7 nm and a PdI of 0.245 ± 0.010, indicating homogeneity in particle size and distribution. The positive ZP (+28.06 ± 1.10 mV) suggested the presence of chitosan on the nanocapsule surfaces, making the suspension more acidic (pH 4.38). EE was high at 89.89 ± 1.29 %. SEM images revealed the spherical/oval shape of the nanocapsules, and AFM confirmed their nanometric size and distribution within the system. Encapsulated TTO and AZI demonstrated lower MIC values compared to their free forms for all tested strains within 24 h, indicating enhanced antimicrobial efficacy. NP-TTO-AZI-CH exhibited bactericidal activity and ... Mostrar Tudo |
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Palavras-Chave: |
Nanotecnologia; Quitosana. |
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Thesagro: |
Óleo Essencial. |
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Thesaurus Nal: |
Anti-infective agents; Essential oils; Nanoparticles; Nanotechnology. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02849naa a2200337 a 4500
001 2177258
005 2025-07-14
008 2025 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.micpath.2025.107820$2DOI
100 1 $aOLIVEIRA, M. S. de
245 $aPoly(ε-caprolactone) nanocapsules coated with Chitosan optimize the antimicrobial activity of Tea Tree Oil and Azithromycin against oral pathogens.$h[electronic resource]
260 $c2025
520 $aThis study aimed to develop poly(ε-caprolactone) nanocapsules coated with chitosan, loaded with Tea Tree Oil (TTO) and Azithromycin (AZI), to optimize the antimicrobial activity and bioavailability of these actives. The nanocapsules suspension (NP-TTO-AZI-CH) was prepared using the preformed polymer interfacial precipitation method and coated with a 2 % chitosan solution. Physicochemical characterization included evaluating the pH, mean hydrodynamic diameter (Z-Average), Zeta potential (ZP), polydispersity index (PdI), encapsulation efficiency (EE) of the essential oil, and morphological assessment by Scanning Electron Microscopy (SEM) and Atomic Force Microscopy (AFM). Biological tests conducted encompassed Minimum Inhibitory Concentration (MIC), Minimum Microbicidal Concentration (MMC), and cell viability assessments. NP-TTO-AZI-CH showed a ZAverage of 238.5 ± 1.7 nm and a PdI of 0.245 ± 0.010, indicating homogeneity in particle size and distribution. The positive ZP (+28.06 ± 1.10 mV) suggested the presence of chitosan on the nanocapsule surfaces, making the suspension more acidic (pH 4.38). EE was high at 89.89 ± 1.29 %. SEM images revealed the spherical/oval shape of the nanocapsules, and AFM confirmed their nanometric size and distribution within the system. Encapsulated TTO and AZI demonstrated lower MIC values compared to their free forms for all tested strains within 24 h, indicating enhanced antimicrobial efficacy. NP-TTO-AZI-CH exhibited bactericidal activity and maintained 70 % cell viability within the first 24 h at concentrations ranging from 0.5 mg/mL + 20 μg/mL to 0.06 mg/mL + 2.5 μg/mL (TTO + AZI). This formulation proved to be a promising tool for enhancing the antimicrobial activity of bioactives and combating various oral microbial strains.
650 $aAnti-infective agents
650 $aEssential oils
650 $aNanoparticles
650 $aNanotechnology
650 $aÓleo Essencial
653 $aNanotecnologia
653 $aQuitosana
700 1 $aSILVA, N. P. da
700 1 $aPAULA, M. S. de
700 1 $aPORTO, D. L.
700 1 $aARAGAO, C. F. S.
700 1 $aSEGURA, M. E. C.
700 1 $aFABRI, R. L.
700 1 $aBEMQUERER, M. P.
700 1 $aBRANDAO, H. de M.
700 1 $aTAVARES, G. D.
700 1 $aAPOLONIO, A. C. M.
773 $tMicrobial Pathogenesis$gv. 206, 107820, 2025.
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Embrapa Gado de Leite (CNPGL) |
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| Registros recuperados : 37 | |
| 9. |  | TRINDADE, M. da G.; CÁNOVAS, A. D.; SÓ e SILVA, M.; SOUSA, A. de. Avaliação do valor de cultivo e uso (VCU1) de genótipos de trigo irrigado nos estados de Goiás e Mato Grosso na safra 2002/2003. In: REUNIÃO DA COMISSÃO CENTRO BRASILEIRA DE PESQUISA DE TRIGO, 13.; SEMINÁRIO TÉCNICO DE TRIGO, 2., 2004, Goiânia. Atas e resumos expandidos. Passo Fundo: Embrapa Trigo, 2006. p. 101-107. (Embrapa Trigo. Documentos, 67).| Biblioteca(s): Embrapa Trigo. |
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| 10. | | TRINDADE, M. da G.; CÁNOVAS, A. D.; SÓ e SILVA, M.; SOUSA, A. de. Avaliação do valor de cultivo e uso (VCU3) de genótipos de trigo irrigado nos estados de Goiás e Mato Grosso na safra 2002/2003. In: REUNIÃO DA COMISSÃO CENTRO BRASILEIRA DE PESQUISA DE TRIGO, 13.; SEMINÁRIO TÉCNICO DE TRIGO, 2., 2004, Goiânia. Atas e resumos expandidos. Passo Fundo: Embrapa Trigo, 2006. p. 108-114. (Embrapa Trigo. Documentos, 67).| Biblioteca(s): Embrapa Trigo. |
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| 19. | | TRINDADE, M. da G.; CÁNOVAS, A. D.; SÓ e SILVA, M.; SOUSA, A. de. Estabilidade e adaptabilidade de genótipos de trigo irrigado nos estados de Goiás e Mato Grosso na safra 2002/2003. In: REUNIÃO DA COMISSÃO CENTRO BRASILEIRA DE PESQUISA DE TRIGO, 13.; SEMINÁRIO TÉCNICO DE TRIGO, 2., 2004, Goiânia. Atas e resumos expandidos. Passo Fundo: Embrapa Trigo, 2006. p. 169-175. (Embrapa Trigo. Documentos, 67).| Biblioteca(s): Embrapa Trigo. |
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| 20. | | FRANÇA, A. M. da S.; SANO, E. E.; SOUSA, A. de O.; FONSECA, R. A. Sensoriamento remoto na identificação e quantificação de áreas úmidas no Distrito Federal. In: SIMPÓSIO NACIONAL CERRADO, 9.; SIMPÓSIO INTERNACIONAL SAVANAS TROPICAIS, 2., 2008, Brasília, DF. Desafios e estratégias para o equilíbrio entre sociedade, agronegócio e recursos naturais: anais... Planaltina, DF: Embrapa Cerrados, 2008. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso / Nota Técnica |
| Biblioteca(s): Embrapa Cerrados. |
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| Registros recuperados : 37 | |
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