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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
12/06/2025 |
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Data da última atualização: |
12/06/2025 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
BASSO, M. B.; RIBEIRO, T. P.; LOURENCO, I. T.; OLIVEIRA, N. G.; MENDES, R. A. G.; FERNANDES, N. A. N.; ARRAES, F. B. M.; MORGANTE, C. V.; HEMERLY, A. S.; SA, M. F. G. de. |
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Afiliação: |
MARCOS FERNANDO BASSO, NATIONAL INSTITUTE OF SCIENCE AND TECHNOLOGY; THUANNE PIRES RIBEIRO, NATIONAL INSTITUTE OF SCIENCE AND TECHNOLOGY; ISABELA TRISTAN LOURENCO TESSUTTI, CENARGEN; NELSON GERALDO OLIVEIRA, NATIONAL INSTITUTE OF SCIENCE AND TECHNOLOGY; RENEIDA APARECIDA GODINHO MENDES, NATIONAL INSTITUTE OF SCIENCE AND TECHNOLOGY; NIDAY ALLINE NUNES FERNANDES; FABRICIO BARBOSA MONTEIRO ARRAES, NATIONAL INSTITUTE OF SCIENCE AND TECHNOLOGY; CAROLINA VIANNA MORGANTE, CENARGEN; ADRIANA SILVA HEMERLY, FEDERAL UNIVERSITY OF RIO DE JANEIRO; MARIA FATIMA GROSSI DE SA, CENARGEN. |
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Título: |
RNAi-mediated down-regulation of the endogenous GhAIP10.1 and GhAIP10.2 genes in transgenic cotton (Gossypium hirsutum) enhances the earliness and yield of flower buds. |
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Ano de publicação: |
2025 |
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Fonte/Imprenta: |
Plant Physiology and Biochemistry, v. 225, 109937, 2025. |
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DOI: |
https://doi.org/10.1016/j.plaphy.2025.109937 |
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Idioma: |
Inglês |
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Notas: |
Na publicação: Isabela Tristan Lourenço-Tessutti; Maria Fatima Grossi-de-Sa. |
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Conteúdo: |
Armadillo BTB Arabidopsis protein 1 (AtABAP1) plays a central role in the cell cycle. ABAP1-interacting protein 10 (AtAIP10, a Snf1 kinase interactor-like protein) is a protein that interacts with AtABAP1. Down-regulation of the AtAIP10 gene in A. thaliana resulted in an altered cell cycle and increased photosynthesis, chlorophyll content, metabolites, plant growth, root system, seed yield, and drought tolerance. Herein, aimed to test whether the down-regulation of GhAIP10 genes can stimulate the cotton plants in a manner similar to those observed in A. thaliana. Cotton transgenic events containing transgenes carrying RNA interfering (RNAi) or artificial miRNA (amiRNA) strategies were successfully generated to down-regulate the endogenous GhAIP10.1 and GhAIP10.2 genes. From these 15 transgenic events, five RNAi-based transgenic lines and five amiRNA-based transgenic events were selected for further analyses. The down-regulation of the GhAIP10.1 and GhAIP10.2 genes was confirmed by real-time RT-PCR. Phenotypic and physiological analyses revealed that these transgenic lines exhibited earlier production and opening of flower buds, increased vegetative growth over time and root biomass, no reduction in susceptibility to root-knot nematodes, and improved drought tolerance indicated by a higher photosynthetic rate and better intrinsic water-use efficiency. Based on the high identity of amino acid sequences, motifs, domains, subcellular localization, tertiary structure, down-regulation of GhABAP1 (partner of GhAIP10), up-regulation of GhCdt1 (a marker of the ABAP1 network), up-regulation of GhCyclinB1 (a marker of the cell cycle), up-regulation of GhAP3 (involved in vegetative to reproductive transition), and the up-regulation of CAB3, NDA1, DJC22, and DNAJ11 genes (involved in plant resilience) suggested that GhAIP10.1 and GhAIP10.2 proteins may act in cotton similarly to the AtAIP10 protein in A. thaliana. Furthermore, GhAIP10.1 and GhAIP10.2 genes are suggested as biotechnological targets for cotton genetic engineering based on genome editing. MenosArmadillo BTB Arabidopsis protein 1 (AtABAP1) plays a central role in the cell cycle. ABAP1-interacting protein 10 (AtAIP10, a Snf1 kinase interactor-like protein) is a protein that interacts with AtABAP1. Down-regulation of the AtAIP10 gene in A. thaliana resulted in an altered cell cycle and increased photosynthesis, chlorophyll content, metabolites, plant growth, root system, seed yield, and drought tolerance. Herein, aimed to test whether the down-regulation of GhAIP10 genes can stimulate the cotton plants in a manner similar to those observed in A. thaliana. Cotton transgenic events containing transgenes carrying RNA interfering (RNAi) or artificial miRNA (amiRNA) strategies were successfully generated to down-regulate the endogenous GhAIP10.1 and GhAIP10.2 genes. From these 15 transgenic events, five RNAi-based transgenic lines and five amiRNA-based transgenic events were selected for further analyses. The down-regulation of the GhAIP10.1 and GhAIP10.2 genes was confirmed by real-time RT-PCR. Phenotypic and physiological analyses revealed that these transgenic lines exhibited earlier production and opening of flower buds, increased vegetative growth over time and root biomass, no reduction in susceptibility to root-knot nematodes, and improved drought tolerance indicated by a higher photosynthetic rate and better intrinsic water-use efficiency. Based on the high identity of amino acid sequences, motifs, domains, subcellular localization, tertiary structure, down-regula... Mostrar Tudo |
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Palavras-Chave: |
ABAP1-Interact protein 10; Chaperone DnaJ; Flower earliness. |
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Thesaurus Nal: |
Cell cycle; Drought tolerance; RNA interference. |
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Categoria do assunto: |
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Marc: |
LEADER 03199naa a2200325 a 4500
001 2176582
005 2025-06-12
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024 7 $ahttps://doi.org/10.1016/j.plaphy.2025.109937$2DOI
100 1 $aBASSO, M. B.
245 $aRNAi-mediated down-regulation of the endogenous GhAIP10.1 and GhAIP10.2 genes in transgenic cotton (Gossypium hirsutum) enhances the earliness and yield of flower buds.$h[electronic resource]
260 $c2025
500 $aNa publicação: Isabela Tristan Lourenço-Tessutti; Maria Fatima Grossi-de-Sa.
520 $aArmadillo BTB Arabidopsis protein 1 (AtABAP1) plays a central role in the cell cycle. ABAP1-interacting protein 10 (AtAIP10, a Snf1 kinase interactor-like protein) is a protein that interacts with AtABAP1. Down-regulation of the AtAIP10 gene in A. thaliana resulted in an altered cell cycle and increased photosynthesis, chlorophyll content, metabolites, plant growth, root system, seed yield, and drought tolerance. Herein, aimed to test whether the down-regulation of GhAIP10 genes can stimulate the cotton plants in a manner similar to those observed in A. thaliana. Cotton transgenic events containing transgenes carrying RNA interfering (RNAi) or artificial miRNA (amiRNA) strategies were successfully generated to down-regulate the endogenous GhAIP10.1 and GhAIP10.2 genes. From these 15 transgenic events, five RNAi-based transgenic lines and five amiRNA-based transgenic events were selected for further analyses. The down-regulation of the GhAIP10.1 and GhAIP10.2 genes was confirmed by real-time RT-PCR. Phenotypic and physiological analyses revealed that these transgenic lines exhibited earlier production and opening of flower buds, increased vegetative growth over time and root biomass, no reduction in susceptibility to root-knot nematodes, and improved drought tolerance indicated by a higher photosynthetic rate and better intrinsic water-use efficiency. Based on the high identity of amino acid sequences, motifs, domains, subcellular localization, tertiary structure, down-regulation of GhABAP1 (partner of GhAIP10), up-regulation of GhCdt1 (a marker of the ABAP1 network), up-regulation of GhCyclinB1 (a marker of the cell cycle), up-regulation of GhAP3 (involved in vegetative to reproductive transition), and the up-regulation of CAB3, NDA1, DJC22, and DNAJ11 genes (involved in plant resilience) suggested that GhAIP10.1 and GhAIP10.2 proteins may act in cotton similarly to the AtAIP10 protein in A. thaliana. Furthermore, GhAIP10.1 and GhAIP10.2 genes are suggested as biotechnological targets for cotton genetic engineering based on genome editing.
650 $aCell cycle
650 $aDrought tolerance
650 $aRNA interference
653 $aABAP1-Interact protein 10
653 $aChaperone DnaJ
653 $aFlower earliness
700 1 $aRIBEIRO, T. P.
700 1 $aLOURENCO, I. T.
700 1 $aOLIVEIRA, N. G.
700 1 $aMENDES, R. A. G.
700 1 $aFERNANDES, N. A. N.
700 1 $aARRAES, F. B. M.
700 1 $aMORGANTE, C. V.
700 1 $aHEMERLY, A. S.
700 1 $aSA, M. F. G. de
773 $tPlant Physiology and Biochemistry$gv. 225, 109937, 2025.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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| Registros recuperados : 11 | |
| 1. |  | DUARTE, J. O. Situação da cultura do sorgo no Brasil. In: EMBRAPA. Centro de Nacional de Pesquisa de Milho e Sorgo. Relatório técnico anual do Centro Nacional de Pesquisa de Milho e Sorgo: período 1992-1993. Sete Lagoas, 1994. v. 6, p. 13-14.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 5. | | DUARTE, J. O.; MONTEIRO, J. A.; MIRANDA, J. E.; VIANA, A. C. Comparação de custos de produção de silagens de milho, sorgo e capim elefante, o caso de Sete Lagoas-MG. In: CONGRESSO NACIONAL DE MILHO E SORGO, 21., 1996, Londrina. Resumos... Londrina: IAPAR, 1996. p. 333.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 7. | | VIANA, A. C.; FERREIRA, J. J.; MIRANDA, J. E. C.; VALENTE, J. O.; DUARTE, J. O.; HORTA, M. L. Efeito de silagens de diferentes cultivares de milho, de sorgo e de capim elefante no desempenho de novilhos confinados. In: CONGRESSO NACIONAL DE MILHO E SORGO, 20., 1994, Goiânia. Centro-Oeste: cinturão do milho e do sorgo no Brasil: resumos. Sete Lagoas: ABMS, 1994. p. 193.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 9. | | AMARAL, T. A.; ANDRADE, C. L. T.; DUARTE, J. O.; GARCIA, J. C.; GARCIA Y GARCIA, A.; SILVA, D. F.; ALBERNAZ, W. M.; HOOGENBOOM, G. Nitrogen management strategies for smallholder maize production systems: yield and profitability variability. International Journal of Plant Production, v. 9, n. 1, p. 75-98, 2015.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 10. | | CAPALBO, D. M. F.; SIMON, M. F.; NODARI, R. O.; VALLE, S.; SANTOS, R. F. dos; CORADIN, L.; DUARTE, J. O.; MIRANDA, J. E.; DIAS, E. P. F.; QUYEN, L. Q.; UNDERWOOD, E.; NELSON, K. C. Consideration of problem formulation and option assessment for Bt cotton in Brazil. In: HILBECK, A.; ANDOW, D. A. FONTES, E. M. G. Methodologies for assessing Bt cotton in Brazil. In: KAPUSCINSKI, A. R.; SCHEI, P. J. Environmmental risk assessment of genetically modified organisms. WALLINGFORD: CABI PUBLISHING, 2006. v. 2, P. 67-92.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 11. | | SCHAFFERT, R. E.; ALBUQUERQUE, P. E. P.; DUARTE, J. O.; GARCIA, J. C.; GOMIDE, R. L.; GUIMARAES, C. T.; MAGALHAES, P. C.; MAGALHAES, J. V.; QUEIROZ, V. A. V. Phenotyping sorghum for adaptation to drought. In: MONNEVEUX, P.; RIBAUT, J.-M. (Ed.). Drought phenotyping in crops: from theory to practice. Mexico, DF: CGIAR: CIMMYT, 2011. pt. 2, p. 287-299.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| Registros recuperados : 11 | |
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