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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
10/06/2025 |
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Data da última atualização: |
10/06/2025 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
QUEIROZ, L. N.; MONTEIRO, T. R.; GOMES, A. C. M. M.; CABRAL, G. B.; ARAGÃO, F. J. L. |
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Afiliação: |
LÍDIA N. QUEIROZ, AGROCINCO; TATIANE R. MONTEIRO, UNIVERSIDADE DE BRASÍLIA; ANA CRISTINA MENESES MENDES GOMES, CENARGEN; GLAUCIA BARBOSA CABRAL, CENARGEN; FRANCISCO JOSE LIMA ARAGAO, CENARGEN. |
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Título: |
Enhanced folate content in bioengineered lettuce stably expressed under field conditions. |
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Ano de publicação: |
2025 |
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Fonte/Imprenta: |
Transgenic Research, v. 34,20, 2025. |
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DOI: |
https://doi.org/10.1007/s11248-025-00440-1 |
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Idioma: |
Inglês |
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Conteúdo: |
Folate (vitamin B9) is an essential nutrient that plays a crucial role in various bodily functions. Its deficiency can lead to health issues, such as megaloblastic anemia, neural tube defects in the developing fetus and increased risk of cardiovascular diseases. Consequently, there is interest in increasing folate content in food crops by classical and molecular breeding. Since folate is a water-soluble vitamin that is sensitive to heat, we chose to manipulate its synthesis pathway in lettuce, which is often used for salads and sandwiches, without heat treatment, which makes it a good vehicle for making folate available to the population via biofortification. Transgenic lettuce plants were generated to express the GTP-cyclohydrolase I and aminodeoxychorismate synthase coding sequences from Arabidopsis thaliana. Plants were cultivated under greenhouse conditions and two field trials were carried out. Results have shown that transgenic lines presented up to 3.4 times more folate than the non-transgenic lettuce plants and 1.9 times more folate than spinach, considered one of the plants richest in folates. Advanced generations of homozygous plants were cultivated under field conditions for two years and the results showed that folate-biofortification was stable. A folate-biofortified lettuce serving would provide about 36 to 64% of the recommended daily intake. This technology is a foundation to produce folate-biofortified commercial varieties that can help to reduce hidden hunger, decreasing the number of cases of fetus malformations and other diseases. MenosFolate (vitamin B9) is an essential nutrient that plays a crucial role in various bodily functions. Its deficiency can lead to health issues, such as megaloblastic anemia, neural tube defects in the developing fetus and increased risk of cardiovascular diseases. Consequently, there is interest in increasing folate content in food crops by classical and molecular breeding. Since folate is a water-soluble vitamin that is sensitive to heat, we chose to manipulate its synthesis pathway in lettuce, which is often used for salads and sandwiches, without heat treatment, which makes it a good vehicle for making folate available to the population via biofortification. Transgenic lettuce plants were generated to express the GTP-cyclohydrolase I and aminodeoxychorismate synthase coding sequences from Arabidopsis thaliana. Plants were cultivated under greenhouse conditions and two field trials were carried out. Results have shown that transgenic lines presented up to 3.4 times more folate than the non-transgenic lettuce plants and 1.9 times more folate than spinach, considered one of the plants richest in folates. Advanced generations of homozygous plants were cultivated under field conditions for two years and the results showed that folate-biofortification was stable. A folate-biofortified lettuce serving would provide about 36 to 64% of the recommended daily intake. This technology is a foundation to produce folate-biofortified commercial varieties that can help to reduce hidden hung... Mostrar Tudo |
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Palavras-Chave: |
Folate. |
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Thesaurus Nal: |
Biofortification; Folic acid; Genetic transformation; Lettuce; Malnutrition. |
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Categoria do assunto: |
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Marc: |
LEADER 02319naa a2200253 a 4500
001 2176536
005 2025-06-10
008 2025 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s11248-025-00440-1$2DOI
100 1 $aQUEIROZ, L. N.
245 $aEnhanced folate content in bioengineered lettuce stably expressed under field conditions.$h[electronic resource]
260 $c2025
520 $aFolate (vitamin B9) is an essential nutrient that plays a crucial role in various bodily functions. Its deficiency can lead to health issues, such as megaloblastic anemia, neural tube defects in the developing fetus and increased risk of cardiovascular diseases. Consequently, there is interest in increasing folate content in food crops by classical and molecular breeding. Since folate is a water-soluble vitamin that is sensitive to heat, we chose to manipulate its synthesis pathway in lettuce, which is often used for salads and sandwiches, without heat treatment, which makes it a good vehicle for making folate available to the population via biofortification. Transgenic lettuce plants were generated to express the GTP-cyclohydrolase I and aminodeoxychorismate synthase coding sequences from Arabidopsis thaliana. Plants were cultivated under greenhouse conditions and two field trials were carried out. Results have shown that transgenic lines presented up to 3.4 times more folate than the non-transgenic lettuce plants and 1.9 times more folate than spinach, considered one of the plants richest in folates. Advanced generations of homozygous plants were cultivated under field conditions for two years and the results showed that folate-biofortification was stable. A folate-biofortified lettuce serving would provide about 36 to 64% of the recommended daily intake. This technology is a foundation to produce folate-biofortified commercial varieties that can help to reduce hidden hunger, decreasing the number of cases of fetus malformations and other diseases.
650 $aBiofortification
650 $aFolic acid
650 $aGenetic transformation
650 $aLettuce
650 $aMalnutrition
653 $aFolate
700 1 $aMONTEIRO, T. R.
700 1 $aGOMES, A. C. M. M.
700 1 $aCABRAL, G. B.
700 1 $aARAGÃO, F. J. L.
773 $tTransgenic Research$gv. 34,20, 2025.
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| Registros recuperados : 4 | |
| 1. |  | DUARTE, G. C. A.; COSTA, T. A. D.; RIBEIRO, F. N. S.; HÜTHER, C. M.; LAMEIRA, O. A.; PEREIRA, C. R.; AZEREDO, R. B. V.; MACHADO, E. B. Aplicação de ferramentas quimiométricas na avaliação sazonal da produção metabólica de raízes de Ipecacuanha cultivadas no Leste Fluminense. In: SIMPÓSIO DE PLANTAS MEDICINAIS DO BRASIL, 24., 2016, Belo Horizonte. [Anais]. [Belo Horizonte: CEPLAMT], 2016.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 2. | | DUARTE, G. C. A.; COSTA, T. A. D.; RIBEIRO, F. N. S.; HÜTHER, C. M.; LAMEIRA, O. A.; PEREIRA, C. R.; AZEREDO, R. B. V.; MACHADO, T. B. Avaliação sazonal da produção de emetina e cefalina em raízes de Carapichea ipecacuanha (Rubiaceae): uma espécie em erosão genética. In: JORNADA FLUMINENSE DE PRODUTOS NATURAIS, 3., 2016, Niterói. [Anais]. Niterói: UFF, 2016.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 3. | | SANTOS, C. M. P. P. dos; HUTHER, C. M.; BORELLA, J.; RIBEIRO, F. N. S.; DUARTE, G. C. A.; CARVALHO, L. F. de; OLIVEIRA, E. de; LAMEIRA, O. A.; PINHO, C. F. de; MACHADO, T. de B.; PEREIRA, C. R. Season and shading affect emetine and cephalin production in Carapichea ipecacuanha plants. Plant Biosystems, v. 156, n. 1, p. 51-60, 2022. Published online: 16 Oct 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 4. | | RIBEIRO, F. N. S.; HUTHER, C. M.; OLIVEIRA, J. R. de; CORREIA, D. M.; RODRIGUES, N. F.; ALMEIDA, R. D. B. de; MORAES, J. B. de; SILVA, M. P.; SANTOS, C. M. P. P. dos; PEREIRA, M. de O.; DUARTE, G. C. A.; FERREIRA, V. F.; MACHADO, T. de B.; LAMEIRA, O. A.; CECCHIN, D.; PEREIRA, C. R. Luminosity levels and soil composition influence the growth of Ipecacuanha. Brazilian Journal of Agriculture, v. 97, n. 1, p. 17-31, 2022.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Embrapa Amazônia Oriental. |
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| Registros recuperados : 4 | |
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