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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agroindústria Tropical. Para informações adicionais entre em contato com cnpat.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agroindústria Tropical. |
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Data corrente: |
04/06/2025 |
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Data da última atualização: |
04/06/2025 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CAMPOS, A. S.; JESUS, E. da C.; MENDES FILHO, P. F.; ARAÚJO PEREIRA, A. P. DE; CASTRO, A. C. R. de; COELHO, A. C. M. A.; BORDALLO, P. do N.; BORGES, W. L.; CARVALHO, A. C. P. P. de. |
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Afiliação: |
ARLENE SANTISTEBAN CAMPOS, UNIVERSITY OF SÃO PAULO; EDERSON DA CONCEICAO JESUS, CNPAB; PAULO FURTADO MENDES FILHO, FEDERAL UNIVERSITY OF CEARÁ; ARTHUR PRUDÊNCIO DE ARAÚJO PEREIRA, FEDERAL UNIVERSITY OF CEARÁ; ANA CECILIA RIBEIRO DE CASTRO, CNPAT; ARTHUR CESAR MINA ALBUQUERQUE COELHO, FEDERAL INSTITUTE OF EDUCATION SCIENCE AND TECHNOLOGY OF CEARÁ, PECÉM; PATRICIA DO NASCIMENTO BORDALLO, CNPAT; WARDSSON LUSTRINO BORGES, CNPAT; ANA CRISTINA PORTUGAL P DE CARVALHO, CNPAT. |
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Título: |
A New Multi-Active Heterogeneous Biocatalyst Prepared Through a Layer-by-Layer Co-Immobilization Strategy of Lipase and Laccase on Nanocellulose-Based Materials. |
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Ano de publicação: |
2025 |
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Fonte/Imprenta: |
Applied Soil Ecology, 211, 106102 , 2025. |
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DOI: |
https://doi.org/10.3390/ catal15020099 |
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Idioma: |
Inglês |
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Conteúdo: |
Lipase from Pseudomonas fl uorescens (PFL) and laccase from Trametes versicolor were co-immobilized onto nanocellulose (NC), using a layer-by-layer approach. Initially, PFL was adsorbed onto NC through ionic and hydrophobic interactions. To achieve higher PFL immobilization yield and activity, NC was functionalized with aldehyde groups through periodate oxidation (NCox) or glutaraldehyde activation (NC-GA). FTIR analysis confi rmed these chemical modifi cations. Among the functionalized NCs, NCox showed the best capacity to retain higher amounts of PFL (maximum load: 20 mg/g), and this support was selected to proceed with the co-immobilization experiments. In this process, NCox-250-PFL (NCox activated with 250 µmol/g of aldehyde groups) was covered with polyethyleneimine (PEI), laccase was co-immobilized, and a crosslinking step using glutaraldehyde was used to covalently attach the enzymes to the support, producing the biocatalyst NCox-250-PFL-PEI-Lac-GA. Co-immobilized enzymes presented higher thermal stability (50 ◦ C) than soluble enzymes; co-immobilized laccase retained 61.1% of its activity after 24 h, and PFL retained about 90% after 48 h of deactivation at 50 ◦ C. In operational stability assays, the heterogeneous biocatalysts maintained more than 45% of their activity after fi ve cycles of pNPB hydrolysis and ABTS oxidation. This co-immobilized biocatalyst, with its high stability and activity retention, is a promising multi-active heterogeneous biocatalyst for use in cascade reactions of industrial interest. MenosLipase from Pseudomonas fl uorescens (PFL) and laccase from Trametes versicolor were co-immobilized onto nanocellulose (NC), using a layer-by-layer approach. Initially, PFL was adsorbed onto NC through ionic and hydrophobic interactions. To achieve higher PFL immobilization yield and activity, NC was functionalized with aldehyde groups through periodate oxidation (NCox) or glutaraldehyde activation (NC-GA). FTIR analysis confi rmed these chemical modifi cations. Among the functionalized NCs, NCox showed the best capacity to retain higher amounts of PFL (maximum load: 20 mg/g), and this support was selected to proceed with the co-immobilization experiments. In this process, NCox-250-PFL (NCox activated with 250 µmol/g of aldehyde groups) was covered with polyethyleneimine (PEI), laccase was co-immobilized, and a crosslinking step using glutaraldehyde was used to covalently attach the enzymes to the support, producing the biocatalyst NCox-250-PFL-PEI-Lac-GA. Co-immobilized enzymes presented higher thermal stability (50 ◦ C) than soluble enzymes; co-immobilized laccase retained 61.1% of its activity after 24 h, and PFL retained about 90% after 48 h of deactivation at 50 ◦ C. In operational stability assays, the heterogeneous biocatalysts maintained more than 45% of their activity after fi ve cycles of pNPB hydrolysis and ABTS oxidation. This co-immobilized biocatalyst, with its high stability and activity retention, is a promising multi-active heterogeneous biocatalyst for use ... Mostrar Tudo |
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Palavras-Chave: |
Ativação de glutaraldeído; Co-immobilization; Co-imobilização; Glutaraldehyde activation; Lacase; Nanocellulose; Nanocelulose; Oxidação de periodato; Periodate oxidation. |
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Thesagro: |
Enzima; Lípase. |
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Thesaurus Nal: |
Enzymes; Laccase. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02753naa a2200385 a 4500
001 2176427
005 2025-06-04
008 2025 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.3390/ catal15020099$2DOI
100 1 $aCAMPOS, A. S.
245 $aA New Multi-Active Heterogeneous Biocatalyst Prepared Through a Layer-by-Layer Co-Immobilization Strategy of Lipase and Laccase on Nanocellulose-Based Materials.$h[electronic resource]
260 $c2025
520 $aLipase from Pseudomonas fl uorescens (PFL) and laccase from Trametes versicolor were co-immobilized onto nanocellulose (NC), using a layer-by-layer approach. Initially, PFL was adsorbed onto NC through ionic and hydrophobic interactions. To achieve higher PFL immobilization yield and activity, NC was functionalized with aldehyde groups through periodate oxidation (NCox) or glutaraldehyde activation (NC-GA). FTIR analysis confi rmed these chemical modifi cations. Among the functionalized NCs, NCox showed the best capacity to retain higher amounts of PFL (maximum load: 20 mg/g), and this support was selected to proceed with the co-immobilization experiments. In this process, NCox-250-PFL (NCox activated with 250 µmol/g of aldehyde groups) was covered with polyethyleneimine (PEI), laccase was co-immobilized, and a crosslinking step using glutaraldehyde was used to covalently attach the enzymes to the support, producing the biocatalyst NCox-250-PFL-PEI-Lac-GA. Co-immobilized enzymes presented higher thermal stability (50 ◦ C) than soluble enzymes; co-immobilized laccase retained 61.1% of its activity after 24 h, and PFL retained about 90% after 48 h of deactivation at 50 ◦ C. In operational stability assays, the heterogeneous biocatalysts maintained more than 45% of their activity after fi ve cycles of pNPB hydrolysis and ABTS oxidation. This co-immobilized biocatalyst, with its high stability and activity retention, is a promising multi-active heterogeneous biocatalyst for use in cascade reactions of industrial interest.
650 $aEnzymes
650 $aLaccase
650 $aEnzima
650 $aLípase
653 $aAtivação de glutaraldeído
653 $aCo-immobilization
653 $aCo-imobilização
653 $aGlutaraldehyde activation
653 $aLacase
653 $aNanocellulose
653 $aNanocelulose
653 $aOxidação de periodato
653 $aPeriodate oxidation
700 1 $aJESUS, E. da C.
700 1 $aMENDES FILHO, P. F.
700 1 $aARAÚJO PEREIRA, A. P. DE
700 1 $aCASTRO, A. C. R. de
700 1 $aCOELHO, A. C. M. A.
700 1 $aBORDALLO, P. do N.
700 1 $aBORGES, W. L.
700 1 $aCARVALHO, A. C. P. P. de
773 $tApplied Soil Ecology, 211, 106102 , 2025.
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Registro original: |
Embrapa Agroindústria Tropical (CNPAT) |
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Biblioteca |
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| Registros recuperados : 13 | |
| 3. |  | ABREU, M. S. de; LOPES, M. T. G.; LOPES, R.; RAIZER, M. D. M.; FRAXE, T. de J. P.; AGUIAR, A. V. de. Florescimento e viabilidade de pólen de açaizeiros do Pará na Amazônia Ocidental. In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS, 6., 2020. Recursos genéticos e bioeconomia: inovação para um futuro sustentável: anais. Brasília, DF: Sociedade Brasileira de Recursos Genéticos, 2020. Trabalho 559.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 4. | | MARQUES, M. J.; BEZERRA, C.; TOMAZ, J.; LOPES, R.; WREGE, M. S.; AGUIAR, A. V. de; RAMOS, S.; MENESES, C. H.; FRAXE, T. de J.; LOPES, M. T. Predição da distribuição geográfica das palmeiras Euterpe precatoria e Euterpe oleracea (Arecaceae). In: SIMPÓSIO DE MUDANÇA CLIMÁTICA E CONSERVAÇÃO DE RECURSOS GENÉTICOS NA AMAZÔNIA, 2024, Manaus. Anais... Manaus: UFAM, 2024.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Ocidental; Embrapa Florestas. |
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| 6. | | ALMEIDA, D. P. de; LOPES, R.; BEZERRA, C. de S.; TOMAZ, J. S.; SOUZA, S. F. de; FERREYRA RAMOS, S. L.; MENESES, C. H. S. G.; CAMPOS, J. F.; FRAXE, T. de J. P.; LOPES, M. T. G. Climate change impact on modeling the distribution of ornamental species in Brazilian territory. Interciência, v. 49, n. 9, p. 527-534, Sept. 2024.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
|  |
| 7. | | TOMAZ, J. S.; BEZERRA, C. de S.; MENESES, C. H. S. G.; MARQUES, R. L. S.; FRAXE, T. de J. P.; LOPES, R.; VALENTE, M. S. F.; FERREYRA RAMOS, S. L.; LIMA, S. C. de S.; LOPES, M. T. G. Plant tissues of Stryphnodendron pulcherrimum (Willd.) Hochr. as environmental bioindicators. Polish Journal of Environmental Studies, v. 34, n. 4, p. 4807-4819, 2025.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 4 |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 8. | | CORDEIRO, A. L.; TOMAZ, J. S.; BEZERRA, C. de S.; MENESES, C. H. S. G.; AGUIAR, A. V. de; WREGE, M. S.; FERREYRA RAMOS, S. L.; LOPES, R.; FRAXE, T. de J. P.; LOPES, M. T. G. Prediction of the geographic distribution and conservation of Amazonian palm trees Astrocaryum acaule Mart. and Astrocaryum aculeatum Mart. Revista Árvore, v. 47, e4719, 2023. Título em Português: Predição da distribuição geográfica e conservação das palmeiras amazônicas Astrocaryum acaule Mart. e Astrocaryum aculeatum Mart.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Amazônia Ocidental; Embrapa Florestas. |
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| 9. | | MARQUES, M. J.; BEZERRA, C. de S.; TOMAZ, J. S.; LOPES, R.; WREGE, M. S.; AGUIAR, A. V. de; FERREYRA RAMOS, S. L.; MENESES, C. H. S. G.; FRAXE, T. de J. P.; LOPES, M. T. G. Prediction of geographic distribution and ecological niche modeling of açaí palm trees in the Amazon. Pesquisa Agropecuária Tropical, Goiânia, v. 54, e78108, 2024.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Embrapa Amazônia Ocidental; Embrapa Florestas. |
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| 10. | | FERREYRA RAMOS, S. L.; LOPES, M. T. G.; MENESES, C.; DEQUIGIOVANNI, G.; MACEDO, J. L. V. de; LOPES, R.; SEBBENN, A. M.; SILVA, R. F. da; FRAXE, T. de J. P.; VEASEY, E. A. Natural populations of Astrocaryum aculeatum Meyer in Amazonia: genetic diversity and conservation. Plants, v. 11, n. 21, art. 2957, 2022.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 4 |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 11. | | ALVES, S. R. M.; LOPES, R.; MENESES, C.; VALENTE, M. S. F.; MARTINS, C. C.; FERREYRA RAMOS, S.; OLIVEIRA, I.; FRAXE, T. de J. P.; COSTA, L.; LOPES, M. T. G. Morpho-agronomic characterization, sample size, and plot size for the evaluation of Capsicum chinense genotypes. Horticulturae, v. 8, n. 9, art. 785, 2022.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 5 |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 12. | | RAMOS, S. L. F.; DEQUIGIOVANNI, G.; LOPES, M. T. G.; AGUIAR, A. V. de; LOPES, R.; VEASEY, E. A.; MACEDO, J. L. V. de; ALVES-PEREIRA, A.; FRAXE, T. de J. P.; WREGE, M. S.; GARCIA, J. N. Genetic structure in populations of Euterpe precatoria Mart. in the Brazilian Amazon. Frontiers in Ecology and Evolution, v, 8, article 603448, Jan. 2021.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
| Biblioteca(s): Embrapa Amazônia Ocidental; Embrapa Florestas. |
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| 13. | | WITKOSKI, A. C.; SILVA, A. J. I. da; BRASIL, D. F.; MAMED, F. de A.; PEIXOTO, G. N. A.; FRAXE, T. de J. P.; BATISTA, V. da S.; AZEVEDO, C. R. de; CASTELO BRANCO, F. M.; SILVA, M. do P. S. C.; MELLO, R. Q. de; NODA, H.; CAMPOS, M. A.; SARAGOUSSI, M.; COSTA, S. de S.; LIMA, R. M. B. de; FAZZIONI, W. Investigações sobre a estrutura sócio-econômica da várzea. In: INPA (Manaus, AM). Projeto: Ecologia de áreas alagáveis - várzea e igapó. Manaus, 1982. Relatório anual 1992, p. 40-41.| Tipo: Capítulo em Livro Técnico-Científico |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| Registros recuperados : 13 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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