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Registro Completo |
Biblioteca(s): |
Embrapa Agroenergia. |
Data corrente: |
13/12/2024 |
Data da última atualização: |
27/12/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
LIMA, A. K. O.; SOUZA, L. M. dos S.; REIS, G. F.; TAVARES JUNIOR, A. G.; ARAÚJO, V. H. S.; SANTOS, L. C. dos; SILVA, V. R. P. da; CHORILLI, M.; BRAGA, H. de C.; TADA. D. B.; RIBEIRO, J. A. de A.; RODRIGUES, C. M.; NAKAZATO, G.; MUEHLMANN, L. A.; GARCIA, M. P. |
Afiliação: |
ALAN KELBIS OLIVEIRA LIMA, UNIVERSITY OF BRASILIA; LUCAS MARCELINO DOS SANTOS SOUZA, STATE UNIVERSITY OF LONDRINA; GUILHERME FONSECA REIS, FEDERAL UNIVERSITY OF PARANÁ; ALBERTO GOMES TAVARES JUNIOR, SÃO PAULO STATE UNIVERSITY; VICTOR HUGO SOUSA ARAÚJO, SÃO PAULO STATE UNIVERSITY; LUCAS CARVALHO DOS SANTOS, UNIVERSITY OF BRASÍLIA; VITÓRIA REGINA PEREIRA DA SILVA, UNIVERSITY OF BRASILIA; MARLUS CHORILLI, SÃO PAULO STATE UNIVERSITY; HUGO DE CAMPOS BRAGA, FEDERAL UNIVERSITY OF SÃO PAULO; DAYANE BATISTA TADA, FEDERAL UNIVERSITY OF SÃO PAULO; JOSE ANTONIO DE AQUINO RIBEIRO, CNPAE; CLENILSON MARTINS RODRIGUES, CNPAE; GERSON NAKAZATO, STATE UNIVERSITY OF LONDRINA; LUÍS ALEXANDRE MUEHLMANN, UNIVERSITY OF BRASÍLIA; MÔNICA PEREIRA GARCIA, UNIVERSITY OF BRASILIA. |
Título: |
Synthesis of silver nanoparticles using extracts from different parts of the Paullinia Cupana Kunth Plant: caracterization and in vitro antimicrobial activity. |
Ano de publicação: |
2024 |
Fonte/Imprenta: |
Pharmaceuticals, v. 17, n. 7, 869, 2024. |
DOI: |
https://doi.org/10.3390/ph17070869 |
Idioma: |
Inglês |
Conteúdo: |
The green synthesis of silver nanoparticles (AgNPs) can be developed using safe and environmentally friendly routes, can replace potentially toxic chemical methods, and can increase the scale of production. This study aimed to synthesize AgNPs from aqueous extracts of guarana (Paullinia cupana) leaves and flowers, collected in different seasons of the year, as a source of active biomolecules capable of reducing silver ions (Ag+) and promoting the stabilization of colloidal silver (Ag0). The plant aqueous extracts were characterized regarding their metabolic composition by liquid chromatography coupled to high-resolution mass spectrometry (UHPLC-HRMS/MS), phenolic compound content, and antioxidant potential against free radicals. The synthesized AgNPs were characterized by UV/Vis spectrophotometry, dynamic light scattering (DLS), nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM), and scanning electron microscopy coupled to energy-dispersive X-ray spectrometry (EDX). The results demonstrated that the chemical characterization indicated the presence of secondary metabolites of many classes of compounds in the studied aqueous extracts studied, but alkaloids and flavonoids were predominant, which are widely recognized for their antioxidant capabilities. It was possible to notice subtle changes in the properties of the nanostructures depending on parameters such as seasonality and the part of the plant used, with the AgNPs showing surface plasmon resonance bands between 410 and 420 nm using the leaf extract and between 440 and 460 nm when prepared using the flower extract. Overall, the average hydrodynamic diameters of the AgNPs were similar among the samples (61.98 to 101.6 nm). Polydispersity index remained in the range of 0.2 to 0.4, indicating that colloidal stability did not change with storage time. Zeta potential was above −30 mV after one month of analysis, which is adequate for biological applications. TEM images showed AgNPs with diameters between 40.72 to 48.85 nm and particles of different morphologies. EDX indicated silver content by weight between 24.06 and 28.81%. The synthesized AgNPs exhibited antimicrobial efficacy against various pathogenic microorganisms of clinical and environmental interest, with MIC values between 2.12 and 21.25 µg/mL, which is close to those described for MBC values. Therefore, our results revealed the potential use of a native species of plant from Brazilian biodiversity combined with nanotechnology to produce antimicrobial agents. MenosThe green synthesis of silver nanoparticles (AgNPs) can be developed using safe and environmentally friendly routes, can replace potentially toxic chemical methods, and can increase the scale of production. This study aimed to synthesize AgNPs from aqueous extracts of guarana (Paullinia cupana) leaves and flowers, collected in different seasons of the year, as a source of active biomolecules capable of reducing silver ions (Ag+) and promoting the stabilization of colloidal silver (Ag0). The plant aqueous extracts were characterized regarding their metabolic composition by liquid chromatography coupled to high-resolution mass spectrometry (UHPLC-HRMS/MS), phenolic compound content, and antioxidant potential against free radicals. The synthesized AgNPs were characterized by UV/Vis spectrophotometry, dynamic light scattering (DLS), nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM), and scanning electron microscopy coupled to energy-dispersive X-ray spectrometry (EDX). The results demonstrated that the chemical characterization indicated the presence of secondary metabolites of many classes of compounds in the studied aqueous extracts studied, but alkaloids and flavonoids were predominant, which are widely recognized for their antioxidant capabilities. It was possible to notice subtle changes in the properties of the nanostructures depending on parameters such as seasonality and the part of the plant used, with the AgNPs showing surface plasmon resonan... Mostrar Tudo |
Palavras-Chave: |
Amazon. |
Thesagro: |
Guaraná. |
Thesaurus Nal: |
Nanoparticles; Silver. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1170690/1/Synthesis-of-silver.pdf
|
Marc: |
LEADER 03601naa a2200349 a 4500 001 2170690 005 2024-12-27 008 2024 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.3390/ph17070869$2DOI 100 1 $aLIMA, A. K. O. 245 $aSynthesis of silver nanoparticles using extracts from different parts of the Paullinia Cupana Kunth Plant$bcaracterization and in vitro antimicrobial activity.$h[electronic resource] 260 $c2024 520 $aThe green synthesis of silver nanoparticles (AgNPs) can be developed using safe and environmentally friendly routes, can replace potentially toxic chemical methods, and can increase the scale of production. This study aimed to synthesize AgNPs from aqueous extracts of guarana (Paullinia cupana) leaves and flowers, collected in different seasons of the year, as a source of active biomolecules capable of reducing silver ions (Ag+) and promoting the stabilization of colloidal silver (Ag0). The plant aqueous extracts were characterized regarding their metabolic composition by liquid chromatography coupled to high-resolution mass spectrometry (UHPLC-HRMS/MS), phenolic compound content, and antioxidant potential against free radicals. The synthesized AgNPs were characterized by UV/Vis spectrophotometry, dynamic light scattering (DLS), nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM), and scanning electron microscopy coupled to energy-dispersive X-ray spectrometry (EDX). The results demonstrated that the chemical characterization indicated the presence of secondary metabolites of many classes of compounds in the studied aqueous extracts studied, but alkaloids and flavonoids were predominant, which are widely recognized for their antioxidant capabilities. It was possible to notice subtle changes in the properties of the nanostructures depending on parameters such as seasonality and the part of the plant used, with the AgNPs showing surface plasmon resonance bands between 410 and 420 nm using the leaf extract and between 440 and 460 nm when prepared using the flower extract. Overall, the average hydrodynamic diameters of the AgNPs were similar among the samples (61.98 to 101.6 nm). Polydispersity index remained in the range of 0.2 to 0.4, indicating that colloidal stability did not change with storage time. Zeta potential was above −30 mV after one month of analysis, which is adequate for biological applications. TEM images showed AgNPs with diameters between 40.72 to 48.85 nm and particles of different morphologies. EDX indicated silver content by weight between 24.06 and 28.81%. The synthesized AgNPs exhibited antimicrobial efficacy against various pathogenic microorganisms of clinical and environmental interest, with MIC values between 2.12 and 21.25 µg/mL, which is close to those described for MBC values. Therefore, our results revealed the potential use of a native species of plant from Brazilian biodiversity combined with nanotechnology to produce antimicrobial agents. 650 $aNanoparticles 650 $aSilver 650 $aGuaraná 653 $aAmazon 700 1 $aSOUZA, L. M. dos S. 700 1 $aREIS, G. F. 700 1 $aTAVARES JUNIOR, A. G. 700 1 $aARAÚJO, V. H. S. 700 1 $aSANTOS, L. C. dos 700 1 $aSILVA, V. R. P. da 700 1 $aCHORILLI, M. 700 1 $aBRAGA, H. de C. 700 1 $aTADA. D. B. 700 1 $aRIBEIRO, J. A. de A. 700 1 $aRODRIGUES, C. M. 700 1 $aNAKAZATO, G. 700 1 $aMUEHLMANN, L. A. 700 1 $aGARCIA, M. P. 773 $tPharmaceuticals$gv. 17, n. 7, 869, 2024.
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Embrapa Agroenergia (CNPAE) |
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Registros recuperados : 6 | |
2. |  | GOUVEIA, E. M. F.; SILVA, I. S.; NAKAZATO, G.; ONSELEM, V. J. V.; CORRÊA, R. A. C.; ARAUJO, F. R.; CHANG, M. R. Action of phosphorylated mannanoligosaccharides on immune and hematological responses and fecal consistency of dogs experimentally infected with enteropathogenic Escherichia coli strains. Brazilian Journal of Microbiology, v. 44, n. 2, 499-504 (2013)Biblioteca(s): Embrapa Gado de Corte. |
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3. |  | KOGA, V. L.; MALUTA, R. P.; WANDERLEY D. DA SILVEIRA, W. D.; RIBEIRO, R. A.; HUNGRIA, M.; VESPERO, E. C.; NAKAZATO, G.; KOBAYASHI, R. K. T. Characterization of CMY-2-type betalactamase-producing Escherichia coli isolated from chicken carcasses and human infection in a city of South Brazil. BMC Microbiology, v. 19, n. 174, 2019. 9 p.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Soja. |
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4. |  | LIMA, A. K. O.; SOUZA, L. M. dos S.; REIS, G. F.; TAVARES JUNIOR, A. G.; ARAÚJO, V. H. S.; SANTOS, L. C. dos; SILVA, V. R. P. da; CHORILLI, M.; BRAGA, H. de C.; TADA. D. B.; RIBEIRO, J. A. de A.; RODRIGUES, C. M.; NAKAZATO, G.; MUEHLMANN, L. A.; GARCIA, M. P. Synthesis of silver nanoparticles using extracts from different parts of the Paullinia Cupana Kunth Plant: caracterization and in vitro antimicrobial activity. Pharmaceuticals, v. 17, n. 7, 869, 2024.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
Biblioteca(s): Embrapa Agroenergia. |
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5. |  | SILVA, M. C. da; COSTA, I. C.; NAKAZATO, G.; PINHEIRO, J. W.; LEDUR, M. C.; LOYOLA, W.; VENANCIO, E. J. Innate immunity in chicken lines developed by Embrapa Suínos e Aves: antimicrobial activity of macrophages and serum. Revista Brasileira de Zootecnia, v. 50, n. e20200229, 2021.Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
Biblioteca(s): Embrapa Suínos e Aves. |
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