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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
25/11/2024 |
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Data da última atualização: |
25/11/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ARAÚJO, A. C. de; BRASILEIRO, A. C. M.; MARTINS, A. da C. Q.; GRYNBERG, P.; TOGAWA, R. C.; SARAIVA, M. A. de P.; MILLER, R. N. G.; GUIMARAES, P. M. |
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Afiliação: |
AMANDA CRISTINA DE ARAÚJO, UNIVERSIDADE DE BRASÍLIA; ANA CRISTINA MIRANDA BRASILEIRO, CENARGEN; ANDRESSA DA CUNHA QUINTANA MARTINS; PRISCILA GRYNBERG, CENARGEN; ROBERTO COITI TOGAWA, CENARGEN; MARIO ALFREDO DE PASSOS SARAIVA, CENARGEN; ROBERT NEIL GERARD MILLER, NATIONAL INSTITUTE OF SCIENCE AND TECHNOLOGY - INCT PLANTSTRESS BIOTECH; PATRICIA MESSEMBERG GUIMARAES, CENARGEN. |
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Título: |
Ectopic expression of a truncated NLR gene from wild Arachis enhances resistance to Fusarium oxysporum. |
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Ano de publicação: |
2024 |
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Fonte/Imprenta: |
Frontiers in Plant Science, v. 15, 2024. |
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DOI: |
https://doi.org/10.3389/fpls.2024.1486820 |
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Idioma: |
Inglês |
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Conteúdo: |
Fusarium oxysporum causes devastating vascular wilt diseases in numerous crop species, resulting in substantial yield losses. The Arabidopsis thaliana-F. oxysporum f.sp. conglutinans (FOC) model system enables the identification of meaningful genotype–phenotype correlations and was applied in this study to evaluate the effects of overexpressing an NLR gene (AsTIR19) from Arachis stenosperma against pathogen infection. AsTIR19 overexpression (OE) lines exhibited enhanced resistance to FOC without any discernible phenotype penalties. To elucidate the underlying resistance mechanisms mediated by AsTIR19 overexpression, we conducted whole transcriptome sequencing of an AsTIR19-OE line and non-transgenic wild-type (WT) plants inoculated and non-inoculated with FOC using Illumina HiSeq4000. Comparative analysis revealed 778 differentially expressed genes (DEGs) attributed to transgene overexpression, while fungal inoculation induced 434 DEGs in the OE line, with many falling into defense-related Gene Ontology (GO) categories. GO and KEGG enrichment analysis showed that DEGs were enriched in the phenylpropanoid and flavonoid pathways in the OE plants. This comprehensive transcriptomic analysis underscores how AsTIR19 overexpression reprograms transcriptional networks, modulating the expression of stress-responsive genes across diverse metabolic pathways. These findings provide valuable insights into the molecular mechanisms underlying the role of this NLR gene under stress conditions, highlighting its potential to enhance resistance to Fusarium oxysporum. MenosFusarium oxysporum causes devastating vascular wilt diseases in numerous crop species, resulting in substantial yield losses. The Arabidopsis thaliana-F. oxysporum f.sp. conglutinans (FOC) model system enables the identification of meaningful genotype–phenotype correlations and was applied in this study to evaluate the effects of overexpressing an NLR gene (AsTIR19) from Arachis stenosperma against pathogen infection. AsTIR19 overexpression (OE) lines exhibited enhanced resistance to FOC without any discernible phenotype penalties. To elucidate the underlying resistance mechanisms mediated by AsTIR19 overexpression, we conducted whole transcriptome sequencing of an AsTIR19-OE line and non-transgenic wild-type (WT) plants inoculated and non-inoculated with FOC using Illumina HiSeq4000. Comparative analysis revealed 778 differentially expressed genes (DEGs) attributed to transgene overexpression, while fungal inoculation induced 434 DEGs in the OE line, with many falling into defense-related Gene Ontology (GO) categories. GO and KEGG enrichment analysis showed that DEGs were enriched in the phenylpropanoid and flavonoid pathways in the OE plants. This comprehensive transcriptomic analysis underscores how AsTIR19 overexpression reprograms transcriptional networks, modulating the expression of stress-responsive genes across diverse metabolic pathways. These findings provide valuable insights into the molecular mechanisms underlying the role of this NLR gene under stress conditio... Mostrar Tudo |
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Palavras-Chave: |
Plant defense; Truncated-NLR. |
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Thesagro: |
Stress. |
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Thesaurus Nal: |
Fungi; Transcriptome. |
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Categoria do assunto: |
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Marc: |
LEADER 02404naa a2200277 a 4500
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024 7 $ahttps://doi.org/10.3389/fpls.2024.1486820$2DOI
100 1 $aARAÚJO, A. C. de
245 $aEctopic expression of a truncated NLR gene from wild Arachis enhances resistance to Fusarium oxysporum.$h[electronic resource]
260 $c2024
520 $aFusarium oxysporum causes devastating vascular wilt diseases in numerous crop species, resulting in substantial yield losses. The Arabidopsis thaliana-F. oxysporum f.sp. conglutinans (FOC) model system enables the identification of meaningful genotype–phenotype correlations and was applied in this study to evaluate the effects of overexpressing an NLR gene (AsTIR19) from Arachis stenosperma against pathogen infection. AsTIR19 overexpression (OE) lines exhibited enhanced resistance to FOC without any discernible phenotype penalties. To elucidate the underlying resistance mechanisms mediated by AsTIR19 overexpression, we conducted whole transcriptome sequencing of an AsTIR19-OE line and non-transgenic wild-type (WT) plants inoculated and non-inoculated with FOC using Illumina HiSeq4000. Comparative analysis revealed 778 differentially expressed genes (DEGs) attributed to transgene overexpression, while fungal inoculation induced 434 DEGs in the OE line, with many falling into defense-related Gene Ontology (GO) categories. GO and KEGG enrichment analysis showed that DEGs were enriched in the phenylpropanoid and flavonoid pathways in the OE plants. This comprehensive transcriptomic analysis underscores how AsTIR19 overexpression reprograms transcriptional networks, modulating the expression of stress-responsive genes across diverse metabolic pathways. These findings provide valuable insights into the molecular mechanisms underlying the role of this NLR gene under stress conditions, highlighting its potential to enhance resistance to Fusarium oxysporum.
650 $aFungi
650 $aTranscriptome
650 $aStress
653 $aPlant defense
653 $aTruncated-NLR
700 1 $aBRASILEIRO, A. C. M.
700 1 $aMARTINS, A. da C. Q.
700 1 $aGRYNBERG, P.
700 1 $aTOGAWA, R. C.
700 1 $aSARAIVA, M. A. de P.
700 1 $aMILLER, R. N. G.
700 1 $aGUIMARAES, P. M.
773 $tFrontiers in Plant Science$gv. 15, 2024.
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| 1. |  | MENDES, R. de A.; PEREIRA, A. A. G.; SOUZA FILHO, A. P. da S.; ALVES, S. de M.; BAYMA, J. de C.; SANTOS, L. S. Determinação da atividade alelopática de Brachiaria Humidicula (Gramineae). In: CONGRESSO BRASILEIRO DE QUÍMICA, 39., 1999, Goiânia. Química para o desenvolvimento sustentável: livro de resumos. Goiânia: ABQ, 1999. p. 341.| Tipo: Resumo em Anais de Congresso |
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