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 | Acesso ao texto completo restrito à biblioteca da Embrapa Recursos Genéticos e Biotecnologia. Para informações adicionais entre em contato com cenargen.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
16/10/2024 |
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Data da última atualização: |
16/10/2024 |
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Tipo da produção científica: |
Capítulo em Livro Técnico-Científico |
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Autoria: |
SILVA-CARDOSO, I. M. de A.; MEDEIROS, M. O.; GOMES, A. C. M. M.; SOUZA, A. L. X. de; PEREIRA, J. E. S. |
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Afiliação: |
INAÊ MARIÊ DE ARAÚJO SILVA-CARDOSO; MARIANA OLIVEIRA MEDEIROS, UNIVERSITY OF BRASÍLIA; ANA CRISTINA MENESES MENDES GOMES, CENARGEN; ANDRE LUIS XAVIER DE SOUZA, CENARGEN; JONNY EVERSON SCHERWINSKI PEREIRA, CENARGEN. |
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Título: |
Use of electron microscopy for the detection of contaminant Endophytic bacteria in vitro |
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Ano de publicação: |
2024 |
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Fonte/Imprenta: |
In: LOYOLA-VARGAS, V.; OCHOA-ALEJO, N. (Ed.). Plant cell culture protocols. Springer Nature, 2024. (Methods in Molecular Biology, 2827). Cap. 5. |
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Páginas: |
p. 71-84 |
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Idioma: |
Inglês |
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Notas: |
Na publicação: Jonny Everson Scherwinski-Pereira. |
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Conteúdo: |
The success of in vitro cultivation, particularly for micropropagation purposes, depends on the efficient control of contaminants. In this context, the sterilization of plant material constitutes a fundamental step in initiating cultures. Microbial contaminants can be found either on the surface (epiphyte) or inside plant explants (endophyte). However, the latter is generally challenging to detect and may not always be eradicated through surface sterilization alone. Endophyte contaminants, such as bacteria, can persist within plant material over several cultivation cycles, potentially interfering with or inhibiting in vitro establishment, growth, or recovery of cryopreserved materials. Therefore, microscopy techniques, such as electron microscopy, can yield valuable insights into bacterial endophytes’ localization, tissue colonization patterns, and functions in in vitro plant culture. This information is essential for adopting effective strategies for eliminating, preventing, or harmonious coexistence with contaminants. |
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Palavras-Chave: |
Bacteria contamination; In vitro contamination; Localization. |
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Thesaurus Nal: |
Electron microscopy; Endophytes; Micropropagation. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01961naa a2200265 a 4500
001 2168135
005 2024-10-16
008 2024 bl uuuu u00u1 u #d
100 1 $aSILVA-CARDOSO, I. M. de A.
245 $aUse of electron microscopy for the detection of contaminant Endophytic bacteria in vitro$h[electronic resource]
260 $c2024
300 $ap. 71-84
500 $aNa publicação: Jonny Everson Scherwinski-Pereira.
520 $aThe success of in vitro cultivation, particularly for micropropagation purposes, depends on the efficient control of contaminants. In this context, the sterilization of plant material constitutes a fundamental step in initiating cultures. Microbial contaminants can be found either on the surface (epiphyte) or inside plant explants (endophyte). However, the latter is generally challenging to detect and may not always be eradicated through surface sterilization alone. Endophyte contaminants, such as bacteria, can persist within plant material over several cultivation cycles, potentially interfering with or inhibiting in vitro establishment, growth, or recovery of cryopreserved materials. Therefore, microscopy techniques, such as electron microscopy, can yield valuable insights into bacterial endophytes’ localization, tissue colonization patterns, and functions in in vitro plant culture. This information is essential for adopting effective strategies for eliminating, preventing, or harmonious coexistence with contaminants.
650 $aElectron microscopy
650 $aEndophytes
650 $aMicropropagation
653 $aBacteria contamination
653 $aIn vitro contamination
653 $aLocalization
700 1 $aMEDEIROS, M. O.
700 1 $aGOMES, A. C. M. M.
700 1 $aSOUZA, A. L. X. de
700 1 $aPEREIRA, J. E. S.
773 $tIn: LOYOLA-VARGAS, V.; OCHOA-ALEJO, N. (Ed.). Plant cell culture protocols. Springer Nature, 2024. (Methods in Molecular Biology, 2827). Cap. 5.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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| 1. |  | STENFFEN, R. B.; ANTONIOLLI, Z. I.; BOSEMBECKER, K.; STEFFEN, G. P. K.; CAMPOS, A. D.; GOMES, C. B. Avaliação de óleos essenciais de plantas medicinais no controle de meloidogyne graminicola em arroz irrigado. Nematologia brasileira, Piracicaba, v. 32, n. 2, p.127-135, 2008.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Nacional - A |
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