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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste; Embrapa Suínos e Aves. |
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Data corrente: |
23/08/2024 |
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Data da última atualização: |
23/08/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
LORENZETTI, W. R.; IBELLI, A. M. G.; PEIXOTO, J. de O.; SAVOLDI, I. R.; MORES, M. A. Z.; ROMANO, G. DE S.; CARMO, K. B. DO; LEDUR, M. C. |
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Afiliação: |
WILLIAM RAPHAEL LORENZETTI, UNIVERSIDADE DO ESTADO DE SANTA CATARINA; ADRIANA MERCIA GUARATINI IBELLI, CPPSE; JANE DE OLIVEIRA PEIXOTO, CNPSA; IGOR RICARDO SAVOLDI, UNIVERSIDADE ESTADUAL DE SANTA CATARINA; MARCOS ANTONIO ZANELLA MORES, CNPSA; GABRIELI DE SOUZA ROMANO, UNIVERSIDADE FEDERAL DA BAHIA; KAMILLA BLEIL DO CARMO, UNIVERSIDADE DO CONTESTADO; MONICA CORREA LEDUR, CNPSA. |
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Título: |
The downregulation of genes encoding muscle proteins have a potential role in the development of scrotal hernia in pigs. |
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Ano de publicação: |
2024 |
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Fonte/Imprenta: |
Molecular Biology Reports, v. 51, n. 822, 2024. |
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Páginas: |
12 p. |
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DOI: |
https://doi.org/10.1007/s11033-024-09766-1 |
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Idioma: |
Inglês |
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Conteúdo: |
Background: Testicular descent is a physiological process regulated by many factors. Eventually, disturbances in the embryological/fetal development path facilitate the occurrence of scrotal hernia, a congenital malformation characterized by the presence of intestinal portions within the scrotal sac due to the abnormal expansion of the inguinal ring. In pigs, some genes have been related to this anomaly, but the genetic mechanisms involved remain unclear. This study aimed to investigate the expression profile of a set of genes potentially involved with the manifestation of scrotal hernia in the inguinal ring tissue. Methods: Tissue samples from the inguinal ring/canal of normal and scrotal hernia-affected male pigs with approximately 30 days of age were used. Relative expression analysis was performed using qPCR to confirm the expression profile of 17 candidate genes previously identified in an RNA-Seq study. Among them, the Myosin heavy chain 1 (MYH1), Desmin (DES), and Troponin 1 (TNNI1) genes were differentially expressed between groups and had reduced levels of expression in the affected animals. These genes encode proteins involved in the formation of muscle tissue, which seems to be important for increasing the resistance of the inguinal ring to the abdominal pressure, which is essential to avoid the occurrence of scrotal hernia. Conclusions: The downregulation of muscular candidate genes in the inguinal tissue clarifies the genetic mechanisms involved with this anomaly in its primary site, providing useful information for developing strategies to control this malformation in pigs and other mammals. MenosBackground: Testicular descent is a physiological process regulated by many factors. Eventually, disturbances in the embryological/fetal development path facilitate the occurrence of scrotal hernia, a congenital malformation characterized by the presence of intestinal portions within the scrotal sac due to the abnormal expansion of the inguinal ring. In pigs, some genes have been related to this anomaly, but the genetic mechanisms involved remain unclear. This study aimed to investigate the expression profile of a set of genes potentially involved with the manifestation of scrotal hernia in the inguinal ring tissue. Methods: Tissue samples from the inguinal ring/canal of normal and scrotal hernia-affected male pigs with approximately 30 days of age were used. Relative expression analysis was performed using qPCR to confirm the expression profile of 17 candidate genes previously identified in an RNA-Seq study. Among them, the Myosin heavy chain 1 (MYH1), Desmin (DES), and Troponin 1 (TNNI1) genes were differentially expressed between groups and had reduced levels of expression in the affected animals. These genes encode proteins involved in the formation of muscle tissue, which seems to be important for increasing the resistance of the inguinal ring to the abdominal pressure, which is essential to avoid the occurrence of scrotal hernia. Conclusions: The downregulation of muscular candidate genes in the inguinal tissue clarifies the genetic mechanisms involved with this anomal... Mostrar Tudo |
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Palavras-Chave: |
Anomaly; Congenital malformation; Inguinal ring. |
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Thesaurus Nal: |
Gene expression; Swine. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
null
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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| 1. |  | COSTA JUNIOR, J. C. da C.; MELO, P. L. A. de; PEREIRA, K. T. de O.; SANTOS, T. C. dos; GOMES, T. C. de A.; COSTA, J. G. da. Nutrientes em plantas da caatinga em Planossolos Háplicos de Alagoas. In: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 35., 2015, Natal. O solo e suas múltiplas funções: anais. Natal: Sociedade Brasileira de Ciência do Solo, 2015.| Tipo: Artigo em Anais de Congresso |
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| 2. | | COSTA JUNIOR, J. C. da C.; MELO, P. L. A. de; SILVA, K. B. da; OLIVEIRA, J. D. S. de; GOMES, T. C. de A.; COSTA, J. G. da. Fenóis e flavonoides totais em feijão bravo (Capparis flexuosa) e mororó (Bauhinia cheilantha) em Planossolos Háplicos do semiárido alagoano. In: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 35., 2015, Natal. O solo e suas múltiplas funções: anais. Natal: Sociedade Brasileira de Ciência do Solo, 2015.| Tipo: Artigo em Anais de Congresso |
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