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Registro Completo |
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Biblioteca(s): |
Embrapa Instrumentação; Embrapa Soja. |
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Data corrente: |
08/08/2024 |
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Data da última atualização: |
28/08/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
COUTINHO, I. D.; FACCHINATO, W. M.; MERTZ-HENNING, L. M.; VIANA, A. J. C.; MARIN, S. R. R.; SANTAGNELI, S. H.; NEPOMUCENO, A. L.; COLNAGO, L. A. |
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Afiliação: |
ISABEL DUARTE COUTINHO, INSTITUTO DE QUÍMICA DA UNESP-ARARAQUARA; WILLIAM MARCONDES FACCHINATTO, NSTITUTO DE QUÍMICA DE SÃO CARLOS, UNIVERSIDADE DE SÃO PAULO, IQSC/USP; LILIANE MARCIA MERTZ HENNING, CNPSO; AMÉRICO JOSÉ CARVALHO VIANA, UNIVERSIDADE ESTADUAL DE CAMPINAS (UNICAMP); SILVANA REGINA ROCKENBACH MARIN, CNPSO; SÃO PAULO STATE UNIVERSITY (UNESP); ALEXANDRE LIMA NEPOMUCENO, CNPSO; LUIZ ALBERTO COLNAGO, CNPDIA. |
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Título: |
NMR Fingerprinting of Conventional and Genetically Modified Soybean Plants with AtAREB1 Transcription Factors. |
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Ano de publicação: |
2024 |
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Fonte/Imprenta: |
ACS Omega, v. 9, 2024. |
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Páginas: |
32651−32661 |
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DOI: |
https://doi.org/10.1021/acsomega.4c01796 |
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Idioma: |
Inglês |
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Conteúdo: |
the insertion of the activated form of the AtAREB1 gene in the soybean cultivar BR16, which is sensitive to water deficit, improved the drought response of the genetically modified plants. Thus, in this study, we used 1 H NMR in solution and solid-state NMR to investigate the response of genetically modified soybean overexpressing AtAREB1 under water deficiency conditions. We achieved that drought-tolerant soybean yields high content of amino acids isoleucine, leucine, threonine, valine, proline, glutamate, aspartate, asparagine, tyrosine, and phenylalanine after 12 days of drought stress conditions, as compared to drought-sensitive soybean under the same conditions. Specific target compounds, including sugars, organic acids, and phenolic compounds, were identified as involved in controlling sensitive soybean during the vegetative stage. Solid-state NMR was used to study the impact of drought stress on starch and cellulose contents in different soybean genotypes. The findings provide insights into the metabolic adjustments of soybean overexpressing AREB transcription factors in adapting to dry climates. This study presents NMR techniques for investigating the metabolome of transgenic soybean plants in response to the water deficit. The approach allowed for the identification of physiological and morphological changes in drought-resistant and drought-tolerant soybean tissues. The findings indicate that drought stress significantly alters micro- and macromolecular metabolism in soybean plants. Differential responses were observed among roots and leaves as well as drought-tolerant and drought-sensitive cultivars, highlighting the complex interplay between overexpressed transcription factors and drought stress in soybean plants. Menosthe insertion of the activated form of the AtAREB1 gene in the soybean cultivar BR16, which is sensitive to water deficit, improved the drought response of the genetically modified plants. Thus, in this study, we used 1 H NMR in solution and solid-state NMR to investigate the response of genetically modified soybean overexpressing AtAREB1 under water deficiency conditions. We achieved that drought-tolerant soybean yields high content of amino acids isoleucine, leucine, threonine, valine, proline, glutamate, aspartate, asparagine, tyrosine, and phenylalanine after 12 days of drought stress conditions, as compared to drought-sensitive soybean under the same conditions. Specific target compounds, including sugars, organic acids, and phenolic compounds, were identified as involved in controlling sensitive soybean during the vegetative stage. Solid-state NMR was used to study the impact of drought stress on starch and cellulose contents in different soybean genotypes. The findings provide insights into the metabolic adjustments of soybean overexpressing AREB transcription factors in adapting to dry climates. This study presents NMR techniques for investigating the metabolome of transgenic soybean plants in response to the water deficit. The approach allowed for the identification of physiological and morphological changes in drought-resistant and drought-tolerant soybean tissues. The findings indicate that drought stress significantly alters micro- and macromolecular metabolism i... Mostrar Tudo |
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Palavras-Chave: |
Cultivar BR16. |
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Thesagro: |
Espectrometria; Física; Soja. |
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Thesaurus Nal: |
Nuclear magnetic resonance spectroscopy; Soybeans. |
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Categoria do assunto: |
--
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://www.alice.cnptia.embrapa.br/alice/bitstream/doc/1166368/1/P-NMR-Fingerprinting-of-Conventional-and-Genetically-Modified.pdf
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Marc: |
LEADER 02648naa a2200301 a 4500
001 2166368
005 2024-08-28
008 2024 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1021/acsomega.4c01796$2DOI
100 1 $aCOUTINHO, I. D.
245 $aNMR Fingerprinting of Conventional and Genetically Modified Soybean Plants with AtAREB1 Transcription Factors.$h[electronic resource]
260 $c2024
300 $a32651−32661
520 $athe insertion of the activated form of the AtAREB1 gene in the soybean cultivar BR16, which is sensitive to water deficit, improved the drought response of the genetically modified plants. Thus, in this study, we used 1 H NMR in solution and solid-state NMR to investigate the response of genetically modified soybean overexpressing AtAREB1 under water deficiency conditions. We achieved that drought-tolerant soybean yields high content of amino acids isoleucine, leucine, threonine, valine, proline, glutamate, aspartate, asparagine, tyrosine, and phenylalanine after 12 days of drought stress conditions, as compared to drought-sensitive soybean under the same conditions. Specific target compounds, including sugars, organic acids, and phenolic compounds, were identified as involved in controlling sensitive soybean during the vegetative stage. Solid-state NMR was used to study the impact of drought stress on starch and cellulose contents in different soybean genotypes. The findings provide insights into the metabolic adjustments of soybean overexpressing AREB transcription factors in adapting to dry climates. This study presents NMR techniques for investigating the metabolome of transgenic soybean plants in response to the water deficit. The approach allowed for the identification of physiological and morphological changes in drought-resistant and drought-tolerant soybean tissues. The findings indicate that drought stress significantly alters micro- and macromolecular metabolism in soybean plants. Differential responses were observed among roots and leaves as well as drought-tolerant and drought-sensitive cultivars, highlighting the complex interplay between overexpressed transcription factors and drought stress in soybean plants.
650 $aNuclear magnetic resonance spectroscopy
650 $aSoybeans
650 $aEspectrometria
650 $aFísica
650 $aSoja
653 $aCultivar BR16
700 1 $aFACCHINATO, W. M.
700 1 $aMERTZ-HENNING, L. M.
700 1 $aVIANA, A. J. C.
700 1 $aMARIN, S. R. R.
700 1 $aSANTAGNELI, S. H.
700 1 $aNEPOMUCENO, A. L.
700 1 $aCOLNAGO, L. A.
773 $tACS Omega$gv. 9, 2024.
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Registro original: |
Embrapa Instrumentação (CNPDIA) |
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| Registros recuperados : 14 | |
| 8. |  | ANTONIOLLI, L. R.; CASTRO, P. R. de C. e; KLUGE, R. A.; SCARPARE FILHO, J. A. Remoção da adstringência de frutos de caquizeiro Giombo sob diferentes períodos de exposição ao vapor de álcool etílico. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 35, n. 10, p. 2083-91, out. 2000. Título em inglês: Astringency removal of 'Giombo' persimmon fruit in different exposure periods to ethyl alcohol vapor.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 9. | | ANTONIOLLI, L. R.; CASTRO, P. R. de C. e; KLUGE, R. A.; SCARPARE FILHO, J. A. Remoção da adstringência de frutos de caquizeiro 'Giombo' sob diferentes temperaturas. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 37, n. 5, p. 687-692, maio 2002 Título em inglês: Astringency removal of 'Giombo' persimmon fruits submitted to different temperatures.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 11. | | SILVA, F. C. da; MURAOKA, T.; CASTRO, P. R. de C. e; FREIRE, F. J.; TAVARES, S. Avaliação da adubação nitrogenada e potássica em soqueiras de cana-de-açúcar sem queima. In: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 33., 2011, Uberlândia. Solos nos biomas brasileiros: sustentabilidade e mudanças climáticas: anais. [Uberlândia]: SBCS: UFU, ICIAG, 2011. p. 1-4. 1 CD-ROM.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Agricultura Digital. |
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| 14. | | SILVA, F. C. da; MURAOKA, T.; CASTRO, P. R. de C. e; TAVARES, S.; TRIVELIN, P. C. O.; BROSSI, M. J. de L.; PACKER, J. V. D.; SANTOS, M. B. dos. Avaliação da adubação com nitrogênio e potássio em soqueiras de cana-de-açúcar sem queima. Campinas: Embrapa Informática Agropecuária, 2007. 32 p. (Embrapa Informática Agropecuária. Boletim de pesquisa e desenvolvimento, 16).| Biblioteca(s): Embrapa Agricultura Digital. |
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| Registros recuperados : 14 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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