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Registro Completo |
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Biblioteca(s): |
Embrapa Agroenergia. |
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Data corrente: |
03/07/2024 |
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Data da última atualização: |
03/07/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SERRA, L. A.; MENDES, T. D.; DE MARCO, J. L.; ALMEIDA, J. R. M. de. |
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Afiliação: |
LUANA ASSIS SERRA, UNIVERSITY OF BRASILIA; THAIS DEMARCHI MENDES, CNPAE; JANICE LISBOA DE MARCO, UNIVERSITY OF BRASÍLIA; JOAO RICARDO MOREIRA DE ALMEIDA, CNPAE. |
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Título: |
Application of thermomyces lanuginosus polygalacturonase produced in Komagataella phaffii in biomass hydrolysis and textile bioscouring. |
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Ano de publicação: |
2024 |
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Fonte/Imprenta: |
Enzyme and Microbial Technology, v. 177, 110424, 2024. |
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Idioma: |
Inglês |
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Conteúdo: |
In this work, the polygalacturonase (TL-PG1) from the thermophilic fungus Thermomyces lanuginosus was heterologously produced for the first time in the yeast Komagataella phaffii. The TL-PG1 was successfully expressed under the control of the AOX1 promoter and sequentially purified by His-tag affinity. The purified recombinant pectinase exhibited an activity of 462.6 U/mL toward polygalacturonic acid under optimal conditions (pH 6 and 55 ˚C) with a 2.83 mg/mL and 0.063 μmol/minute for Km and Vmax, respectively. When used as supplementation for biomass hydrolysis, TL-PG1 demonstrated synergy with the enzymatic cocktail Ctec3 to depolymerize orange citrus pulp, releasing 1.43 mg/mL of reducing sugar. In addition, TL-PG1 exhibited efficiency in fabric bioscouring, showing potential usage in the textile industry. Applying a protein dosage of 7 mg/mL, the time for the fabric to absorb water was 19.77 seconds (ten times faster than the control). Adding the surfactant Triton to the treatment allowed the reduction of the enzyme dosage by 50% and the water absorption time to 6.38 seconds. Altogether, this work describes a new versatile polygalacturonase from T. lanuginosus with the potential to be employed in the hydrolysis of lignocellulosic biomass and bioscouring. |
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Thesagro: |
Biomassa; Fungo. |
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Thesaurus Nal: |
Biomass; Bioscouring; Heterologous gene expression; Polygalacturonase. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01993naa a2200229 a 4500
001 2165402
005 2024-07-03
008 2024 bl uuuu u00u1 u #d
100 1 $aSERRA, L. A.
245 $aApplication of thermomyces lanuginosus polygalacturonase produced in Komagataella phaffii in biomass hydrolysis and textile bioscouring.$h[electronic resource]
260 $c2024
520 $aIn this work, the polygalacturonase (TL-PG1) from the thermophilic fungus Thermomyces lanuginosus was heterologously produced for the first time in the yeast Komagataella phaffii. The TL-PG1 was successfully expressed under the control of the AOX1 promoter and sequentially purified by His-tag affinity. The purified recombinant pectinase exhibited an activity of 462.6 U/mL toward polygalacturonic acid under optimal conditions (pH 6 and 55 ˚C) with a 2.83 mg/mL and 0.063 μmol/minute for Km and Vmax, respectively. When used as supplementation for biomass hydrolysis, TL-PG1 demonstrated synergy with the enzymatic cocktail Ctec3 to depolymerize orange citrus pulp, releasing 1.43 mg/mL of reducing sugar. In addition, TL-PG1 exhibited efficiency in fabric bioscouring, showing potential usage in the textile industry. Applying a protein dosage of 7 mg/mL, the time for the fabric to absorb water was 19.77 seconds (ten times faster than the control). Adding the surfactant Triton to the treatment allowed the reduction of the enzyme dosage by 50% and the water absorption time to 6.38 seconds. Altogether, this work describes a new versatile polygalacturonase from T. lanuginosus with the potential to be employed in the hydrolysis of lignocellulosic biomass and bioscouring.
650 $aBiomass
650 $aBioscouring
650 $aHeterologous gene expression
650 $aPolygalacturonase
650 $aBiomassa
650 $aFungo
700 1 $aMENDES, T. D.
700 1 $aDE MARCO, J. L.
700 1 $aALMEIDA, J. R. M. de
773 $tEnzyme and Microbial Technology$gv. 177, 110424, 2024.
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| 2. |  | RODRIGUES, D. B.; TUNES, L. V. M.; VILLELA, F. A.; GADOTTI, G. I.; COSTA, C. J.; ROSA, T. D.; MEDEIROS, D.; GULARTE, J. A.; GEWEHR, E.; ALMEIDA, A. S.; NUNES, C. Production Potential and Quality of Chenopodium quinoa Willd. Seed Cultivated in Different Seeding Seasons. Journal of Agricultural Science, v. 11, n. 1, 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
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