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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
05/06/2024 |
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Data da última atualização: |
05/06/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MEDEIROS, M. O.; SILVA-CARDOSO, I. M. de A.; COSTA, F. H. da S.; QUEIROZ, P. R. M.; ECKSTEIN, B.; SOUZA, A. L. X. de; PEREIRA, J. E. S. |
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Afiliação: |
MARIANA OLIVEIRA MEDEIROS, UNIVERSITY OF BRASÍLIA; INAÊ MARIÊ DE ARAÚJO SILVA-CARDOSO; FREDERICO HENRIQUE DA SILVA COSTA, UNIVERSITY OF ACRE; PAULO ROBERTO MARTINS QUEIROZ; BARBARA ECKSTEIN, CENARGEN; ANDRE LUIS XAVIER DE SOUZA, CENARGEN; JONNY EVERSON SCHERWINSKI PEREIRA, CENARGEN. |
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Título: |
Unveiling innovations for enhancing in vitro propagation of Vanilla phaeantha Rchb.f. through the use of double-phase technique and temporary immersion bioreactor systems. |
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Ano de publicação: |
2024 |
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Fonte/Imprenta: |
Industrial Crops and Products, v. 216, 118726, 2024. |
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DOI: |
https://doi.org/10.1016/j.indcrop.2024.118726 |
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Idioma: |
Português |
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Notas: |
Na publicação: Jonny Everson Scherwinski-Pereira. |
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Conteúdo: |
The study aimed to introduce in vitro propagation techniques for Vanilla phaeantha, focusing on the effectiveness of the double-phase technique and temporary immersion bioreactor systems for clonal multiplication. Optimal conditions for plantlets acclimatization were also sought. Endophytic bacteria occurring in the culture were isolated, analyzed, and controlled. Explants obtained from greenhouse mother plants underwent a decontamination process using sodium ampicillin at different concentrations (0, 250, and 500 mg L−1) for up to five subcultures. Bacteria present during establishment phase were isolated and analyzed via 16 S rRNA sequencing. The study also encompassed an evaluation of clonal multiplication strategies, which included assessing the influence of different consistencies of culture media (liquid, double-phase, and semisolid), and the use of three temporary immersion bioreactor models (RITA®, RALM® and TIB®). The resulting plantlets underwent acclimatization in a mixture of commercial substrate and coconut fiber with varying composition and were kept in a growth chamber before being transferred to a greenhouse. It was verified that the use of ampicillin sodium led to a significant reduction in bacterial contamination over five subcultures. Sequencing using the 16 S rRNA gene enabled the identification of some contaminant bacteria, including species of the genera Rhizobium, Herbaspirillum, and Methylobacterium. Clonal multiplication strategies were evaluated, with the double-phase method proving superior in shoot production per explant (7.0 ± 0.4) compared to the semisolid medium (5.3 ± 0.3). Among temporary immersion bioreactor models tested, RALM® was most effective in shoot formation when compared to RITA® and TIB® (8.2 ± 0.3; 4.9 ± 0.2, and 1.2 ± 0.1, respectively). The use of substrates composed of commercial substrate and coconut fiber, in any combination tested, ensuring 100 % survival of vanilla plantlets in the greenhouse. The findings provide a micropropagation protocol for Vanilla phaeantha, offering a diversification avenue for vanilla cultivation. MenosThe study aimed to introduce in vitro propagation techniques for Vanilla phaeantha, focusing on the effectiveness of the double-phase technique and temporary immersion bioreactor systems for clonal multiplication. Optimal conditions for plantlets acclimatization were also sought. Endophytic bacteria occurring in the culture were isolated, analyzed, and controlled. Explants obtained from greenhouse mother plants underwent a decontamination process using sodium ampicillin at different concentrations (0, 250, and 500 mg L−1) for up to five subcultures. Bacteria present during establishment phase were isolated and analyzed via 16 S rRNA sequencing. The study also encompassed an evaluation of clonal multiplication strategies, which included assessing the influence of different consistencies of culture media (liquid, double-phase, and semisolid), and the use of three temporary immersion bioreactor models (RITA®, RALM® and TIB®). The resulting plantlets underwent acclimatization in a mixture of commercial substrate and coconut fiber with varying composition and were kept in a growth chamber before being transferred to a greenhouse. It was verified that the use of ampicillin sodium led to a significant reduction in bacterial contamination over five subcultures. Sequencing using the 16 S rRNA gene enabled the identification of some contaminant bacteria, including species of the genera Rhizobium, Herbaspirillum, and Methylobacterium. Clonal multiplication strategies were evaluated, wi... Mostrar Tudo |
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Palavras-Chave: |
Endophytic contamination; Large-scale micropropagation; Liquid medium; Vanilla. |
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Thesagro: |
Orchidaceae. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03115naa a2200277 a 4500
001 2164682
005 2024-06-05
008 2024 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.indcrop.2024.118726$2DOI
100 1 $aMEDEIROS, M. O.
245 $aUnveiling innovations for enhancing in vitro propagation of Vanilla phaeantha Rchb.f. through the use of double-phase technique and temporary immersion bioreactor systems.$h[electronic resource]
260 $c2024
500 $aNa publicação: Jonny Everson Scherwinski-Pereira.
520 $aThe study aimed to introduce in vitro propagation techniques for Vanilla phaeantha, focusing on the effectiveness of the double-phase technique and temporary immersion bioreactor systems for clonal multiplication. Optimal conditions for plantlets acclimatization were also sought. Endophytic bacteria occurring in the culture were isolated, analyzed, and controlled. Explants obtained from greenhouse mother plants underwent a decontamination process using sodium ampicillin at different concentrations (0, 250, and 500 mg L−1) for up to five subcultures. Bacteria present during establishment phase were isolated and analyzed via 16 S rRNA sequencing. The study also encompassed an evaluation of clonal multiplication strategies, which included assessing the influence of different consistencies of culture media (liquid, double-phase, and semisolid), and the use of three temporary immersion bioreactor models (RITA®, RALM® and TIB®). The resulting plantlets underwent acclimatization in a mixture of commercial substrate and coconut fiber with varying composition and were kept in a growth chamber before being transferred to a greenhouse. It was verified that the use of ampicillin sodium led to a significant reduction in bacterial contamination over five subcultures. Sequencing using the 16 S rRNA gene enabled the identification of some contaminant bacteria, including species of the genera Rhizobium, Herbaspirillum, and Methylobacterium. Clonal multiplication strategies were evaluated, with the double-phase method proving superior in shoot production per explant (7.0 ± 0.4) compared to the semisolid medium (5.3 ± 0.3). Among temporary immersion bioreactor models tested, RALM® was most effective in shoot formation when compared to RITA® and TIB® (8.2 ± 0.3; 4.9 ± 0.2, and 1.2 ± 0.1, respectively). The use of substrates composed of commercial substrate and coconut fiber, in any combination tested, ensuring 100 % survival of vanilla plantlets in the greenhouse. The findings provide a micropropagation protocol for Vanilla phaeantha, offering a diversification avenue for vanilla cultivation.
650 $aOrchidaceae
653 $aEndophytic contamination
653 $aLarge-scale micropropagation
653 $aLiquid medium
653 $aVanilla
700 1 $aSILVA-CARDOSO, I. M. de A.
700 1 $aCOSTA, F. H. da S.
700 1 $aQUEIROZ, P. R. M.
700 1 $aECKSTEIN, B.
700 1 $aSOUZA, A. L. X. de
700 1 $aPEREIRA, J. E. S.
773 $tIndustrial Crops and Products$gv. 216, 118726, 2024.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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