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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
29/01/2024 |
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Data da última atualização: |
30/01/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OLIVEIRA, K. C. B. F.; FARIAS, E. A. de O.; TEIXEIRA, P. R. S.; BRANDÃO, V. S.; SÁBIO, R. M.; ARAÚJO, A. R. de; EATON, P.; BERTOLINO, L. C.; BEMQUERER, M. P.; BARUD, H. da S.; LEITE, J. R. de S. de A.; EIRAS, C. |
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Afiliação: |
KARLA COSTA BEZERRA FONTENELE OLIVEIRA, UNIVERSIDADE FEDERAL DO PIAUÍ; EMANUEL AIRTON DE OLIVEIRA FARIAS, UNIVERSIDADE FEDERAL DO PIAUÍ; PAULO RONALDO SOUSA TEIXEIRA, UNIVERSIDADE FEDERAL DO PIAUÍ; VITOR SCHWENCK BRANDÃO, CENTRO DE TECNOLOGIA MINERAL; RAFAEL MIGUEL SÁBIO, UNIVERSIDADE DE ARARAQUARA; ALYNE RODRIGUES DE ARAÚJO, UNIVERSIDADE FEDERAL DO PIAUÍ; PETER EATON, UNIVERSIDADE DO PORTO; LUIZ CARLOS BERTOLINO, CENTRO DE TECNOLOGIA MINERAL; MARCELO PORTO BEMQUERER, CNPGL; HERNANE DA SILVA BARUD, UNIVERSIDADE DE ARARAQUARA; JOSÉ ROBERTO DE SOUZA DE ALMEIDA LEITE, UNIVERSIDADE DE BRASÍLIA; CARLA EIRAS, UNIVERSIDADE FEDERAL DO PIAUÍ. |
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Título: |
Development of a nanostructured film containing palygorskite and dermaseptin 01 peptide for biotechnological applications. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Clays and Clay Minerals, v. 71, p. 600-615, 2023. |
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DOI: |
https://doi.org/10.1007/s42860-023-00259-z |
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Idioma: |
Inglês |
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Conteúdo: |
Clay minerals are suitable matrices to anchor organic molecules such as antimicrobial peptides (AMPs) so that their bioactivity is maintained, enabling the formation of new materials with potential for new applications in biotechnology. The objective of the present study was to develop a nanostructured flm where the properties of palygorskite (Plg) were combined at the molecular level with Dermaseptin 01 (DRS 01), in which the clay mineral also served as a substrate for the immobilization of this peptide. The flms were prepared using the Layer-byLayer (LbL) self-assembly technique. Crude palygorskite without purifcation (Plg IN) was subjected to physical and chemical procedures to increase its adsorptive properties. The structure, chemical composition, and morphology of Plg were investigated by X-ray difraction (XRD), Fourier-transform infrared spectroscopy (FTIR), X-ray fuorescence spectrometry (XRF), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). LbL flms were adsorbed onto ITO (Indium Tin Oxide) and characterized electrochemically by cyclic voltammetry (CV), UV-Visible spectroscopy, and atomic force microscopy (AFM). For the ITO/DRS 01 and ITO/Plg/DRS 01 flms, an oxidation process at +0.77 V was observed, confrming that the DRS 01 maintained its electroactive behavior and intrinsic properties. The results also showed that Plg served as excellent support for the immobilization of DRS 01, increasing its concentration and availability in the flm form. This work reported immobilizing the DRS 01 peptide with Plg for the frst time in an ultrathin flm with bioactive properties. Thus, the flm developed can be explored for applications such as biosensor devices and antimicrobial coating materials as well as other biotechnological applications. MenosClay minerals are suitable matrices to anchor organic molecules such as antimicrobial peptides (AMPs) so that their bioactivity is maintained, enabling the formation of new materials with potential for new applications in biotechnology. The objective of the present study was to develop a nanostructured flm where the properties of palygorskite (Plg) were combined at the molecular level with Dermaseptin 01 (DRS 01), in which the clay mineral also served as a substrate for the immobilization of this peptide. The flms were prepared using the Layer-byLayer (LbL) self-assembly technique. Crude palygorskite without purifcation (Plg IN) was subjected to physical and chemical procedures to increase its adsorptive properties. The structure, chemical composition, and morphology of Plg were investigated by X-ray difraction (XRD), Fourier-transform infrared spectroscopy (FTIR), X-ray fuorescence spectrometry (XRF), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). LbL flms were adsorbed onto ITO (Indium Tin Oxide) and characterized electrochemically by cyclic voltammetry (CV), UV-Visible spectroscopy, and atomic force microscopy (AFM). For the ITO/DRS 01 and ITO/Plg/DRS 01 flms, an oxidation process at +0.77 V was observed, confrming that the DRS 01 maintained its electroactive behavior and intrinsic properties. The results also showed that Plg served as excellent support for the immobilization of DRS 01, increasing its concentration and availability in the ... Mostrar Tudo |
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Palavras-Chave: |
Cationic antimicrobial peptides; Fibrous clay minerals; Filme fino; Filme nanoestruturado; Mineral argiloso fibroso; Peptídeo antimicrobiano catiônico; Pithecopus hipocondria. |
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Thesagro: |
Biotecnologia; Peptídeo. |
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Categoria do assunto: |
W Química e Física |
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Marc: |
LEADER 02987naa a2200373 a 4500
001 2161436
005 2024-01-30
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s42860-023-00259-z$2DOI
100 1 $aOLIVEIRA, K. C. B. F.
245 $aDevelopment of a nanostructured film containing palygorskite and dermaseptin 01 peptide for biotechnological applications.$h[electronic resource]
260 $c2023
520 $aClay minerals are suitable matrices to anchor organic molecules such as antimicrobial peptides (AMPs) so that their bioactivity is maintained, enabling the formation of new materials with potential for new applications in biotechnology. The objective of the present study was to develop a nanostructured flm where the properties of palygorskite (Plg) were combined at the molecular level with Dermaseptin 01 (DRS 01), in which the clay mineral also served as a substrate for the immobilization of this peptide. The flms were prepared using the Layer-byLayer (LbL) self-assembly technique. Crude palygorskite without purifcation (Plg IN) was subjected to physical and chemical procedures to increase its adsorptive properties. The structure, chemical composition, and morphology of Plg were investigated by X-ray difraction (XRD), Fourier-transform infrared spectroscopy (FTIR), X-ray fuorescence spectrometry (XRF), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). LbL flms were adsorbed onto ITO (Indium Tin Oxide) and characterized electrochemically by cyclic voltammetry (CV), UV-Visible spectroscopy, and atomic force microscopy (AFM). For the ITO/DRS 01 and ITO/Plg/DRS 01 flms, an oxidation process at +0.77 V was observed, confrming that the DRS 01 maintained its electroactive behavior and intrinsic properties. The results also showed that Plg served as excellent support for the immobilization of DRS 01, increasing its concentration and availability in the flm form. This work reported immobilizing the DRS 01 peptide with Plg for the frst time in an ultrathin flm with bioactive properties. Thus, the flm developed can be explored for applications such as biosensor devices and antimicrobial coating materials as well as other biotechnological applications.
650 $aBiotecnologia
650 $aPeptídeo
653 $aCationic antimicrobial peptides
653 $aFibrous clay minerals
653 $aFilme fino
653 $aFilme nanoestruturado
653 $aMineral argiloso fibroso
653 $aPeptídeo antimicrobiano catiônico
653 $aPithecopus hipocondria
700 1 $aFARIAS, E. A. de O.
700 1 $aTEIXEIRA, P. R. S.
700 1 $aBRANDÃO, V. S.
700 1 $aSÁBIO, R. M.
700 1 $aARAÚJO, A. R. de
700 1 $aEATON, P.
700 1 $aBERTOLINO, L. C.
700 1 $aBEMQUERER, M. P.
700 1 $aBARUD, H. da S.
700 1 $aLEITE, J. R. de S. de A.
700 1 $aEIRAS, C.
773 $tClays and Clay Minerals$gv. 71, p. 600-615, 2023.
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Embrapa Gado de Leite (CNPGL) |
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| Registros recuperados : 12 | |
| 4. |  | ARRIEL, N. H. C.; ANDRADE, F. P. de; FARIAS, F. J. C.; COSTA, I, T. da; GUEDES, A. R. Aderencia placental das sementes e componentes de producao em progenies de gergelim. Revista de Oleaginosas e Fibrosas, v.2,n.2, p.133-139,1998.| Biblioteca(s): Embrapa Algodão. |
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| 5. | | ARRIEL, N. H. C.; ANDRADE, F. P. de; FARIAS, F. J. C.; COSTA, I. T. da; GUEDES, A. R. Aderencia placentral das sementes componentes de producao em progenies de gergelim. Revista de Oleaginosas e Fibrosas, Campina Grande, v.2,n.2,p.133-139,1998.| Biblioteca(s): Embrapa Algodão. |
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| 12. | | ARRIEL, N. H. C.; ANDRADE, F. P. de; COUTINHO, J. L. B.; BOUTY, F. de A. C.; AMIM, S. M. F.; GUEDES, A. R.; COSTA, I. T. da; COSTA, L. B. da; ALENCAR, A. R. de. Competição de genótipos de gergelim em quatro estados do Nordeste do Brasil -1995. Campina Grande : Embrapa Algodão, 1998. 5 p. (Embrapa Algodão. Pesquisa em Andamento, 74).| Biblioteca(s): Embrapa Algodão. |
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| Registros recuperados : 12 | |
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