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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
28/12/2023 |
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Data da última atualização: |
22/01/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ROSA, P. M. da S.; GUEDES, P. H. E.; GARCIA, J. M.; OLIVEIRA, C. S. |
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Afiliação: |
PAOLA MARIA DA SILVA ROSA, UNIVERSIDADE ESTADUAL PAULISTA; PEDRO HENRIQUE EVAGELISTA GUEDES; JOAQUIM MANSANO GARCIA, UNIVERSIDADE ESTADUAL PAULISTA; CLARA SLADE OLIVEIRA, CNPGL. |
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Título: |
Cytoplasmic granules in bovine oocytes do not affect embryonic or fetal development. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Zygote, v. 32, n. 1, p. 28-37, 2023. |
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DOI: |
https://doi.org/10.1017/S0967199423000576 |
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Idioma: |
Inglês |
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Conteúdo: |
Oocyte cytoplasmic evaluation is based on homogeneity and granular appearance. Our study investigated if a granular cytoplasm, highly heterogeneous, would affect oocyte competence in bovine. In two experiments, bovine cumulus–oocyte complexes (COCs) with homogeneous cytoplasm (control, CC) and granulated cytoplasm (granular, GC) were selected from a regular pool of COCs. Experiment 1 was performed with slaughterhouse ovaries, and Experiment 2 was carried out in Girolando COCs obtained from ovum pick-up. Granular oocytes had higher caspase 3 levels (66.17 ± 11.61 vs 172.08 ± 16.95, P < 0.01) and similar GAP junction activity (5.64 ± 0.45 vs 6.29 ± 0.29). ZAR1 relative mRNA amount was lower in granular oocytes (178.27 ± 151.63 vs 0.89 ± 0.89, P = 0.01) and no effect was detected for MATER, PPP2R1A, ENY2, IGF2R, and BMP15 genes. Despite molecular differences, no detrimental effect was detected on oocyte competence in GC oocytes. Cleavage (Experiment 1: 59.52 ± 7.21% vs 59.79 ± 6.10% and Experiment 2: 68.88 ± 4.82 vs 74.41 ± 5.89%) and blastocyst (Experiment 1: 29.28 ± 4.14% vs 23.15 ± 2.96% and Experiment 2: 21.11 ± 3.28% vs 21.02 ± 6.08%) rates were similar between CC and GC (Experiments 1 and 2, respectively). Post-transfer embryo development revealed that pregnancy (CC: 24.27 ± 9.70% vs GC: 26.31 ± 7.23%) and calving (23.68% vs 33.33%) rates and fetal growth were not affected by the presence of cytoplasmic granules. Our results demonstrated that oocytes with granular cytoplasm present equivalent efficiency for IVF and calf production compared with homogenous cytoplasm oocytes. This could be observed through similar cleavage, blastocyst rates, and fetal growth development. In addition to differences in oocyte gene expression related to oocyte quality, it seems not to affect oocyte developmental competence. MenosOocyte cytoplasmic evaluation is based on homogeneity and granular appearance. Our study investigated if a granular cytoplasm, highly heterogeneous, would affect oocyte competence in bovine. In two experiments, bovine cumulus–oocyte complexes (COCs) with homogeneous cytoplasm (control, CC) and granulated cytoplasm (granular, GC) were selected from a regular pool of COCs. Experiment 1 was performed with slaughterhouse ovaries, and Experiment 2 was carried out in Girolando COCs obtained from ovum pick-up. Granular oocytes had higher caspase 3 levels (66.17 ± 11.61 vs 172.08 ± 16.95, P < 0.01) and similar GAP junction activity (5.64 ± 0.45 vs 6.29 ± 0.29). ZAR1 relative mRNA amount was lower in granular oocytes (178.27 ± 151.63 vs 0.89 ± 0.89, P = 0.01) and no effect was detected for MATER, PPP2R1A, ENY2, IGF2R, and BMP15 genes. Despite molecular differences, no detrimental effect was detected on oocyte competence in GC oocytes. Cleavage (Experiment 1: 59.52 ± 7.21% vs 59.79 ± 6.10% and Experiment 2: 68.88 ± 4.82 vs 74.41 ± 5.89%) and blastocyst (Experiment 1: 29.28 ± 4.14% vs 23.15 ± 2.96% and Experiment 2: 21.11 ± 3.28% vs 21.02 ± 6.08%) rates were similar between CC and GC (Experiments 1 and 2, respectively). Post-transfer embryo development revealed that pregnancy (CC: 24.27 ± 9.70% vs GC: 26.31 ± 7.23%) and calving (23.68% vs 33.33%) rates and fetal growth were not affected by the presence of cytoplasmic granules. Our results demonstrated that oocytes with granular cytopla... Mostrar Tudo |
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Palavras-Chave: |
Classificação morfológica; IVF; Oócito; Ooplasma; Ovócito. |
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Thesagro: |
Bovino; Citoplasma; Embrião Animal; Granulo. |
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Thesaurus Nal: |
Cytoplasm; Granules. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02681naa a2200301 a 4500
001 2160283
005 2024-01-22
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1017/S0967199423000576$2DOI
100 1 $aROSA, P. M. da S.
245 $aCytoplasmic granules in bovine oocytes do not affect embryonic or fetal development.$h[electronic resource]
260 $c2023
520 $aOocyte cytoplasmic evaluation is based on homogeneity and granular appearance. Our study investigated if a granular cytoplasm, highly heterogeneous, would affect oocyte competence in bovine. In two experiments, bovine cumulus–oocyte complexes (COCs) with homogeneous cytoplasm (control, CC) and granulated cytoplasm (granular, GC) were selected from a regular pool of COCs. Experiment 1 was performed with slaughterhouse ovaries, and Experiment 2 was carried out in Girolando COCs obtained from ovum pick-up. Granular oocytes had higher caspase 3 levels (66.17 ± 11.61 vs 172.08 ± 16.95, P < 0.01) and similar GAP junction activity (5.64 ± 0.45 vs 6.29 ± 0.29). ZAR1 relative mRNA amount was lower in granular oocytes (178.27 ± 151.63 vs 0.89 ± 0.89, P = 0.01) and no effect was detected for MATER, PPP2R1A, ENY2, IGF2R, and BMP15 genes. Despite molecular differences, no detrimental effect was detected on oocyte competence in GC oocytes. Cleavage (Experiment 1: 59.52 ± 7.21% vs 59.79 ± 6.10% and Experiment 2: 68.88 ± 4.82 vs 74.41 ± 5.89%) and blastocyst (Experiment 1: 29.28 ± 4.14% vs 23.15 ± 2.96% and Experiment 2: 21.11 ± 3.28% vs 21.02 ± 6.08%) rates were similar between CC and GC (Experiments 1 and 2, respectively). Post-transfer embryo development revealed that pregnancy (CC: 24.27 ± 9.70% vs GC: 26.31 ± 7.23%) and calving (23.68% vs 33.33%) rates and fetal growth were not affected by the presence of cytoplasmic granules. Our results demonstrated that oocytes with granular cytoplasm present equivalent efficiency for IVF and calf production compared with homogenous cytoplasm oocytes. This could be observed through similar cleavage, blastocyst rates, and fetal growth development. In addition to differences in oocyte gene expression related to oocyte quality, it seems not to affect oocyte developmental competence.
650 $aCytoplasm
650 $aGranules
650 $aBovino
650 $aCitoplasma
650 $aEmbrião Animal
650 $aGranulo
653 $aClassificação morfológica
653 $aIVF
653 $aOócito
653 $aOoplasma
653 $aOvócito
700 1 $aGUEDES, P. H. E.
700 1 $aGARCIA, J. M.
700 1 $aOLIVEIRA, C. S.
773 $tZygote$gv. 32, n. 1, p. 28-37, 2023.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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| Registros recuperados : 7 | |
| 2. |  | MACEDO, R. S.; MADELLA, M.; CALEGARI, M. R.; TEIXEIRA, W. G.; MARTINS, G. C.; VIDAL-TORRADO, P. Coleção de referência de fitólitos de plantas como subsídio para identificação e classificação de fitólitos em solos. In: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 35., 2015, Natal. O solo e suas múltiplas funções: anais. Natal: Sociedade Brasileira de Ciência do Solo, 2015.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 3. | | MACEDO, R. S.; MADELLA, M.; CALEGARI, M. R.; TEIXEIRA, W. G.; MARTINS, G. C.; VIDAL-TORRADO, P. Coleção de referência de fitólitos de plantas como subsídio para identificação e classificação de fitólitos em solos. In: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 35., 2015, Natal. O solo e suas múltiplas funções: anais. Natal: Sociedade Brasileira de Ciência do Solo, 2015.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Solos. |
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| 4. | | MACEDO, R. S.; MADELLA, M.; CALEGARI, M. R.; TEIXEIRA, W. G.; MARTINS, G. C.; VIDAL-TORRADO, P. Fitólitos como subsídio para estudos de reconstrução da vegetação e indicador de mudanças hidrológicas em solos tropicais. In: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 35., 2015, Natal. O solo e suas múltiplas funções: anais. Natal: Sociedade Brasileira de Ciência do Solo, 2015.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 5. | | MACEDO, R. S.; MADELLA, M.; CALEGARI, M. R.; TEIXEIRA, W. G.; MARTINS, G. C.; VIDAL-TORRADO, P. Fitólitos como subsídio para estudos de reconstrução da vegetação e indicador de mudanças hidrológicas em solos tropicais. In: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 35., 2015, Natal. O solo e suas múltiplas funções: anais. Natal: Sociedade Brasileira de Ciência do Solo, 2015.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Solos. |
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| 6. | | SOUZA, J. G. de; ALCAINA-MATEOS, J.; LANCELOTTI, C.; VIDAL-TORRADO, P.; CALEGARI, M. R.; TEIXEIRA, W. G.; MARTINS, G. C.; MACEDO, R. S.; MADELLA, M. Spatial analysis of Amazonian Dark Earth formation supports an anthropic origin at the Caldeirão site, Brazil. Journal of Archaeological Science, v. 176, 106169, 2025.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Amazônia Ocidental; Embrapa Solos. |
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| 7. | | LOMBARDO, U.; ARROYO-KALIN, M.; SCHMIDT, M.; HUISMAN, H.; LIMA, H. P.; MORAES, M. de P.; NEVES, E. G.; CLEMENT, C. R.; FONSECA, J. A. da; ALMEIDA, F. O. de; ALHO, C. F. B. V.; RAMSEY, C. B.; BROWN, G. G.; CAVALLINI, M. S.; COSTA, M. L. da; CUNHA, L.; ANJOS, L. H. C. dos; DENEVAN, W. M.; FAUSTO, C.; CAROMANO, C. F.; FONTANA, A.; FRANCHETTO, B.; GLASER, B.; HECKENBERGER, M. J.; HECHT, S.; HONORATO, V.; JAROSCH, K. A.; JUNQUEIRA, A. B.; KATER, T.; TAMANAHA, E. K.; KUYPER, T. W.; LEHMANN, J.; MADELLA, M.; MAEZUMI, S. Y.; CASCON, L. M.; MAYLE, F. E.; MCKEY, D.; MORAES, B.; MORCOTE-RÍOS, G.; BARBOSA, C. A. P.; MAGALHÃES, M. P.; PRESTES-CARNEIRO, G.; PUGLIESE, F.; PUPIM, F. N.; RACZKA, M. F.; PY-DANIEL, A. R.; ROCHA, B. C. da; RODRIGUES, L.; ROSTAIN, S.; MACEDO, R. S.; SHOCK, M. P.; SPRAFKE, T.; BASSI, F. S.; VALLE, R.; VIDAL-TORRADO, P.; VILLAGRÁN, X. S.; WATLING, J.; WEBER, S. L.; TEIXEIRA, W. G. Evidence confirms an anthropic origin of Amazonian Dark Earths. Nature Communications, v. 13, n. 3444, 2022. 6 p.| Tipo: Nota Técnica/Nota Científica |
| Biblioteca(s): Embrapa Florestas; Embrapa Solos. |
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| Registros recuperados : 7 | |
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