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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agroenergia. Para informações adicionais entre em contato com biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Acre; Embrapa Agroenergia; Embrapa Gado de Corte. |
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Data corrente: |
19/10/2023 |
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Data da última atualização: |
24/03/2025 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OLIVEIRA, J. C. de; SILVA, A. L. D. da; SILVA, L. M. da; FORMIGHIERI, E. F.; PETERS, L. P.; ASSIS, G. M. L. de; SILVA, C. C. da; SOUZA, A. P. de; CAMPOS, T. de. |
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Afiliação: |
JÔNATAS CHAGAS DE OLIVEIRA, UNIVERSIDADE FEDERAL DO ACRE; ANDRÉ LUCAS DOMINGOS DA SILVA, UNIVERSIDADE FEDERAL DO ACRE; LUCIELIO MANOEL DA SILVA, CPATC; EDUARDO FERNANDES FORMIGHIERI, CNPAE; LEILA PRISCILA PETERS, UNIVERSIDADE FEDERAL DO ACRE; GISELLE MARIANO LESSA DE ASSIS, CPAF-AC; CARLA CRISTINA DA SILVA, UNIVERSIDADE DE CAMPINAS; ANETE PEREIRA DE SOUZA, UNIVERSIDADE DE CAMPINAS; TATIANA DE CAMPOS, CPAF-AC. |
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Título: |
Novel microsatellite markers derived from Arachis pintoi transcriptome sequencing for cross-species transferability and varietal identification. |
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Ano de publicação: |
2024 |
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Fonte/Imprenta: |
Plant Molecular Biology Reporter, v. 42, n. 1, p. 183-192, Mar. 2024. |
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DOI: |
https://doi.org/10.1007/s11105-023-01402-9 |
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Idioma: |
Inglês |
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Conteúdo: |
Forage peanut (Arachis pintoi) is an important leguminous forage that has gained popularity due to increased livestock productivity. Furthermore, the species helps with soil fertility and the restoration of degraded areas. However, A. pintoi has a limited number of molecular markers. The objective of this study was to create and characterize gene-derived microsatellite markers as well as to test their transferability to the peanut (Arachis hypogaea) and other six wild Arachis species. A total of 4461 putative simple sequence repeats (SSR) were identified, and PCR primer pairs were designed for 999 SSR regions after filtering out primers with the same annealing site and searching for sequences related to open reading frames (ORFs). The dinucleotide motif was the most common (628; 62.86%). For validation, 186 primer pairs were chosen at random, of which 63 (33.87%) were polymorphic, with an average of 7.37 alleles per locus. Polymorphic information content (PIC=0.70) and discriminatory power (D=0.80) were both high on average. The functional annotation discovered 120 sequences that were assigned to 87 gene ontology functional groups divided into three main categories: molecular function (27 sub-categories), cellular components (21 sub-categories), and biological process (39 sub-categories). Thirty-three SSRs were tested for transferability to peanut and six other wild Arachis species, resulting in variable cross-species amplification (63.64 to 100%). Here, we present the first gene-derived SSR for A. pintoi. These new informative microsatellites may be linked to agronomically important genes to be used in genetic studies. MenosForage peanut (Arachis pintoi) is an important leguminous forage that has gained popularity due to increased livestock productivity. Furthermore, the species helps with soil fertility and the restoration of degraded areas. However, A. pintoi has a limited number of molecular markers. The objective of this study was to create and characterize gene-derived microsatellite markers as well as to test their transferability to the peanut (Arachis hypogaea) and other six wild Arachis species. A total of 4461 putative simple sequence repeats (SSR) were identified, and PCR primer pairs were designed for 999 SSR regions after filtering out primers with the same annealing site and searching for sequences related to open reading frames (ORFs). The dinucleotide motif was the most common (628; 62.86%). For validation, 186 primer pairs were chosen at random, of which 63 (33.87%) were polymorphic, with an average of 7.37 alleles per locus. Polymorphic information content (PIC=0.70) and discriminatory power (D=0.80) were both high on average. The functional annotation discovered 120 sequences that were assigned to 87 gene ontology functional groups divided into three main categories: molecular function (27 sub-categories), cellular components (21 sub-categories), and biological process (39 sub-categories). Thirty-three SSRs were tested for transferability to peanut and six other wild Arachis species, resulting in variable cross-species amplification (63.64 to 100%). Here, we present the first... Mostrar Tudo |
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Palavras-Chave: |
Amendoim forrageiro; Anotação funcional; Cacahuetes forrajeros; Fitomejoramiento; Forage peanut; Identificación de especies; Marcadores genéticos; Repeticiones de microsatélite; RNA-Seq; Transcriptoma; Transferencia de genes. |
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Thesagro: |
Arachis Hypogaea; Genoma; Identificação de Estirpe; Marcador Molecular; Melhoramento Genético Vegetal. |
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Thesaurus Nal: |
Arachis; Arachis pintoi; Forage; Gene transfer; Genetic markers; Genome; Microsatellite repeats; Peanuts; Plant breeding; Species identification; Transcriptome. |
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Categoria do assunto: |
--
G Melhoramento Genético |
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Marc: |
LEADER 03340naa a2200553 a 4500
001 2157490
005 2025-03-24
008 2024 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s11105-023-01402-9$2DOI
100 1 $aOLIVEIRA, J. C. de
245 $aNovel microsatellite markers derived from Arachis pintoi transcriptome sequencing for cross-species transferability and varietal identification.$h[electronic resource]
260 $c2024
520 $aForage peanut (Arachis pintoi) is an important leguminous forage that has gained popularity due to increased livestock productivity. Furthermore, the species helps with soil fertility and the restoration of degraded areas. However, A. pintoi has a limited number of molecular markers. The objective of this study was to create and characterize gene-derived microsatellite markers as well as to test their transferability to the peanut (Arachis hypogaea) and other six wild Arachis species. A total of 4461 putative simple sequence repeats (SSR) were identified, and PCR primer pairs were designed for 999 SSR regions after filtering out primers with the same annealing site and searching for sequences related to open reading frames (ORFs). The dinucleotide motif was the most common (628; 62.86%). For validation, 186 primer pairs were chosen at random, of which 63 (33.87%) were polymorphic, with an average of 7.37 alleles per locus. Polymorphic information content (PIC=0.70) and discriminatory power (D=0.80) were both high on average. The functional annotation discovered 120 sequences that were assigned to 87 gene ontology functional groups divided into three main categories: molecular function (27 sub-categories), cellular components (21 sub-categories), and biological process (39 sub-categories). Thirty-three SSRs were tested for transferability to peanut and six other wild Arachis species, resulting in variable cross-species amplification (63.64 to 100%). Here, we present the first gene-derived SSR for A. pintoi. These new informative microsatellites may be linked to agronomically important genes to be used in genetic studies.
650 $aArachis
650 $aArachis pintoi
650 $aForage
650 $aGene transfer
650 $aGenetic markers
650 $aGenome
650 $aMicrosatellite repeats
650 $aPeanuts
650 $aPlant breeding
650 $aSpecies identification
650 $aTranscriptome
650 $aArachis Hypogaea
650 $aGenoma
650 $aIdentificação de Estirpe
650 $aMarcador Molecular
650 $aMelhoramento Genético Vegetal
653 $aAmendoim forrageiro
653 $aAnotação funcional
653 $aCacahuetes forrajeros
653 $aFitomejoramiento
653 $aForage peanut
653 $aIdentificación de especies
653 $aMarcadores genéticos
653 $aRepeticiones de microsatélite
653 $aRNA-Seq
653 $aTranscriptoma
653 $aTransferencia de genes
700 1 $aSILVA, A. L. D. da
700 1 $aSILVA, L. M. da
700 1 $aFORMIGHIERI, E. F.
700 1 $aPETERS, L. P.
700 1 $aASSIS, G. M. L. de
700 1 $aSILVA, C. C. da
700 1 $aSOUZA, A. P. de
700 1 $aCAMPOS, T. de
773 $tPlant Molecular Biology Reporter$gv. 42, n. 1, p. 183-192, Mar. 2024.
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Registro original: |
Embrapa Acre (CPAF-AC) |
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| Registros recuperados : 10 | |
| 4. |  | CRAVEIRO, A. G.; SILVA, J. F. V.; CARNEIRO, G. E. S.; DEL PELOSO, M. J.; FREIRE FILHO, F. R. Avaliação da resistência de genótipos de feijão (Phaseolus vulgaris) e caupi (Vigna unguiculata) à Meloidogyne javanica. In: CONGRESSO BRASILEIRO DE SOJA, 2.; MERCOSOJA 2002, 2002, Foz do Iguaçu. Perspectivas do agronegócio da soja: resumos. Londrina: Embrapa Soja, 2002. p.301. (Embrapa Soja. Documentos, 181). Organizado por Odilon Ferreira Saraiva, Clara Beatriz Hoffmann-Campo.| Biblioteca(s): Embrapa Meio-Norte; Embrapa Soja. |
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| 5. | | RIBEIRO, N. R.; SILVA, J. F. V.; MEIRELLES, W. F.; CRAVEIRO, A. G.; PARENTONI, S. N.; SANTOS, F. G. dos. Avaliação da resistência de genótipos de milho, sorgo e milheto a Meloidogyne javanica e M. incognita raça 3. Revista Brasileira de Milho e Sorgo, Sete Lagoas, v. 1, n. 3, p. 102-106, set./dez. 2002.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 6. | | CRAVEIRO, A. G.; SILVA, J. F. V.; CARNEIRO, G. E. S.; DEL PELOSO, M. J.; FREIRE FILHO, F. R. Evaluation of beans and cowpea genotypes for resistance to Meloidogyne javanica. Nematology, Boston, v. 4, n. 2, p. 228-229, 2002. Presented in Fourth International Congress of Nematology. Programme and abstracts. 8-13 June 2002, Tenbel, La Galletas, Arona, Tenerife, Canary Islands, Spain.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Soja. |
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| 7. | | RIBEIRO, N. R.; CRAVEIRO, A. G.; SILVA, J. F. V.; FRANCISCO, A.; GOMES, J.; MEIRELLES, W. F. Avaliação de genótipos de milho (Zea mays) aos nematóides Meloidogyne javanica e M. incognita raca 3. In: CONGRESSO NACIONAL DE MILHO E SORGO, 24., 2002, Florianópolis. Meio ambiente e a nova agenda para o agronegócio de milho e sorgo: [resumos expandidos]. Sete Lagoas: ABMS; Embrapa Milho e Sorgo; Epagri, 2002. 1 CD-ROM. Trabpesq.- 4433| Biblioteca(s): Embrapa Soja. |
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| 8. | | RIBEIRO, N. R.; CRAVEIRO, A. G.; SILVA, J. F. V.; FRANCISCO, A.; GOMES, J.; MEIRELLES, W. F. Avaliação de genótipos de milho (Zea mays) aos nematóides Meloidogyne javanica e M. incognita raça 3. In: CONGRESSO NACIONAL DE MILHO E SORGO, 24., 2002, Florianópolis, SC. Meio ambiente e a nova agenda para o agronegócio de milho e sorgo: [resumos expandidos]. Sete Lagoas: ABMS: Embrapa Milho e Sorgo; Florianópolis: Epagri, 2002.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 9. | | CRAVEIRO, A. G.; RIBEIRO, N. R.; SILVA, J. F. V.; CARNEIRO, G. E. S.; DEL PELOSO, M. J.; FREIRE FILHO, F. R. Avaliação da resistência de cultivares de feijão (Phaseolus vulgaris) e caupi (Vigna unguiculata) a Meloidogyne javanica. In: CONGRESSO NACIONAL DE PESQUISA DE FEIJÃO, 7., 2002, Viçosa, MG. Resumos expandidos. Viçosa: UFV; DFT, 2002. p. 111-113.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Arroz e Feijão. |
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| 10. | | SPOLIDORO, R. M.; GALIAN, C. E.; AUDY, J. L. N.; PAIXÃO CORTES, Z. M.; ANDRADE, M.; ARANHA, J. A. S.; AVINO, J. C.; BARON, R.; BERMÚDEZ, L. A.; BORDEAUX-REGO, A. C. G.; BRAGA COELHO, J. R.; DIAS, J. M. C. de S.; CERIONI, A.; CHIAPANI-SOUTO, M.; COLLERE, V.; CRAVEIRO, A.; DEFILIPPI, G.; SOUSA JUNIOR, R. T. de; FARIA, R. S.; FEIJO BARROSO, L.; FELLOWS, L.; FISCHER, H. A.; GARGIONE, L. A.; GODINHO, M. J. de F.; GRACIOSA, H. M. M.; GUIMARÃES, L.; GURGEL DO AMARAL, E.; HAMERA, A. A.; JACOBO, L. A.; KAKUTA, S.; LATTMANN, J. C. H.; LAHORGUE, M. A.; LOBÃO, M. W. N.; LOTUFO, R.; MATOS, W. S.; MAZAROLLO, C. F.; MOLINA, A.; MOSCHETTA, R. A.; NUNES, R. A.; OLIVEIRA, F. J. O.; PEREYRA, H. L.; POZENATO, J. C.; PRODANOV, C. C.; PUTTINI, R. S.; RAUPP, M. A.; RUMPF, R.; SABOYA, F.; SILVA, F. A.; SOTUYO, J.; TOMÁS, D. C. M.; TONHOLO, J.; VIOLA, I.; VIOLATO, C. A.; ZAMPIERI, N. L. V.; ZORZI, I. New horizons for science and technology parks: a brazilian - argentinean perspective. In: IASP WORLD CONFERENCE ON SCIENCE AND TECHNOLOGY PARKS, 27., 2010, Daedeok Innopolis, Korea. Proceedings... [Malaga], IASP, 2010. il., color.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Agroenergia. |
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| Registros recuperados : 10 | |
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