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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
19/07/2023 |
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Data da última atualização: |
19/07/2023 |
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Tipo da produção científica: |
Nota Técnica/Nota Científica |
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Autoria: |
AZEVEDO, T. C.; GUIMARAES, F. F. M.; TRINDADE, R. dos S.; LANA, U. G. de P.; SOUZA, I. R. P. de. |
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Afiliação: |
TÁCILA CRISTINA AZEVEDO, Universidade Federal de São João del-Rei; FLÁVIA FERREIRA MENDES GUIMARAES, Faculdades Ciências da Vida; ROBERTO DOS SANTOS TRINDADE, CNPMS; UBIRACI GOMES DE PAULA LANA, CNPMS; ISABEL REGINA PRAZERES DE SOUZA, CNPMS. |
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Título: |
Comparison of protocols for chromosome duplication in haploid maize. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Revista Brasileira de Milho e Sorgo, v. 22, e1311, 2023. |
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DOI: |
https://doi.org/10.18512/rbms2023v22e1311 |
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Idioma: |
Inglês |
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Conteúdo: |
Different methods of chromosome duplication are used to obtain doubled haploids in maize; however, comparisons of efficiency among protocols have only begun to appear in the literature. Therefore, this study aimed to compare the efficiency of three protocols of chromosome duplication. A randomized block experimental design was used, with three replications composed of three treatments of chromosome duplication (duplication by colchicine injection in plants, duplication by immersion of roots in colchicine solution, and duplication by immersion of newly germinated seedlings in colchicine solution). Two hundred seventy haploid seeds were obtained from crossing the genotype source 91500212 with the haploid inducer hybrid Tail P1 × Tail P2, with 90 seeds for each treatment. In the chromosome duplication through injection, a colchicine solution at 0.125% was applied to the stem of the seedlings 15 days after germination. In the chromosome duplication through root immersion, 15 days after germination, the roots of the seedlings were immersed in a 1% colchicine solution for six hours, followed by removing colchicine residues and transplanting them in trays with the substrate. Finally, in the chromosome duplication of newly germinated seedlings, three days old seedlings were immersed in a 0.06% colchicine solution for 12 hours, then removed from the solution and transplanted in trays with the substrate. Twenty days after treatment, the plants were transplanted in 20-liter pots with fertilized soil in a greenhouse, beginning data collection. Results indicate that the injection protocol showed better survival, better practicality of application, and lower costs and risks. MenosDifferent methods of chromosome duplication are used to obtain doubled haploids in maize; however, comparisons of efficiency among protocols have only begun to appear in the literature. Therefore, this study aimed to compare the efficiency of three protocols of chromosome duplication. A randomized block experimental design was used, with three replications composed of three treatments of chromosome duplication (duplication by colchicine injection in plants, duplication by immersion of roots in colchicine solution, and duplication by immersion of newly germinated seedlings in colchicine solution). Two hundred seventy haploid seeds were obtained from crossing the genotype source 91500212 with the haploid inducer hybrid Tail P1 × Tail P2, with 90 seeds for each treatment. In the chromosome duplication through injection, a colchicine solution at 0.125% was applied to the stem of the seedlings 15 days after germination. In the chromosome duplication through root immersion, 15 days after germination, the roots of the seedlings were immersed in a 1% colchicine solution for six hours, followed by removing colchicine residues and transplanting them in trays with the substrate. Finally, in the chromosome duplication of newly germinated seedlings, three days old seedlings were immersed in a 0.06% colchicine solution for 12 hours, then removed from the solution and transplanted in trays with the substrate. Twenty days after treatment, the plants were transplanted in 20-liter pots with f... Mostrar Tudo |
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Palavras-Chave: |
Duplo haploide; Production of lines. |
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Thesagro: |
Linhagem; Milho; Zea Mays. |
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Thesaurus Nal: |
Doubled haploids. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1155039/1/Comparison-of-protocols-for-chromosome-duplication-in-haploid-maize.pdf
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Marc: |
LEADER 02438naa a2200253 a 4500
001 2155039
005 2023-07-19
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.18512/rbms2023v22e1311$2DOI
100 1 $aAZEVEDO, T. C.
245 $aComparison of protocols for chromosome duplication in haploid maize.$h[electronic resource]
260 $c2023
520 $aDifferent methods of chromosome duplication are used to obtain doubled haploids in maize; however, comparisons of efficiency among protocols have only begun to appear in the literature. Therefore, this study aimed to compare the efficiency of three protocols of chromosome duplication. A randomized block experimental design was used, with three replications composed of three treatments of chromosome duplication (duplication by colchicine injection in plants, duplication by immersion of roots in colchicine solution, and duplication by immersion of newly germinated seedlings in colchicine solution). Two hundred seventy haploid seeds were obtained from crossing the genotype source 91500212 with the haploid inducer hybrid Tail P1 × Tail P2, with 90 seeds for each treatment. In the chromosome duplication through injection, a colchicine solution at 0.125% was applied to the stem of the seedlings 15 days after germination. In the chromosome duplication through root immersion, 15 days after germination, the roots of the seedlings were immersed in a 1% colchicine solution for six hours, followed by removing colchicine residues and transplanting them in trays with the substrate. Finally, in the chromosome duplication of newly germinated seedlings, three days old seedlings were immersed in a 0.06% colchicine solution for 12 hours, then removed from the solution and transplanted in trays with the substrate. Twenty days after treatment, the plants were transplanted in 20-liter pots with fertilized soil in a greenhouse, beginning data collection. Results indicate that the injection protocol showed better survival, better practicality of application, and lower costs and risks.
650 $aDoubled haploids
650 $aLinhagem
650 $aMilho
650 $aZea Mays
653 $aDuplo haploide
653 $aProduction of lines
700 1 $aGUIMARAES, F. F. M.
700 1 $aTRINDADE, R. dos S.
700 1 $aLANA, U. G. de P.
700 1 $aSOUZA, I. R. P. de
773 $tRevista Brasileira de Milho e Sorgo$gv. 22, e1311, 2023.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
12/03/2015 |
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Data da última atualização: |
14/03/2016 |
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Tipo da produção científica: |
Circular Técnica |
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Autoria: |
MEYER, M. C.; CAMPOS, H. D.; GODOY, C. V.; UTIAMADA, C. M.; MACHADO, A. Q.; PIMENTA, C. B.; CASSETARI NETO, D.; JACCOUD FILHO, D. S.; BORGES, E. P.; SIQUERI, F. V.; JULIATTI, F. C.; NUNES JUNIOR, J.; CARNEIRO, L. C.; SILVA, L. H. C. P. da; MADALOSSO, M.; BALARDIN, R. S.; VENANCIO, W. S. |
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Afiliação: |
MAURICIO CONRADO MEYER, CNPSO; HERCULES D. CAMPOS, UNIRV; CLAUDIA VIEIRA GODOY, CNPSO; CARLOS M. UTIAMADA, TAGRO; ANDREIA Q. MACHADO, UNIVERSIDADE DE VÁRZEA GRANDE; CLÁUDIA B. PIMENTA, EMATER-GO; DANIEL CASSETARI NETO, Universidade Federal de Mato Grosso; DAVID S. JACCOUD FILHO, Universidade Estadual de Ponta Grossa; EDSON P. BORGES, Fundação Chapadão; FABIANO V. SIQUERI, Fundação Mato Grosso; FERNANDO C. JULIATTI, Universidade Federal de Uberlândia; JOSÉ NUNES JUNIOR, CTPA; LUCIANA C. CARNEIRO, Universidade Federal de Goiás; LUIS HENRIQUE C. P. DA SILVA, Universidade de Rio Verde; MARCELO MADALOSSO, Instituto Phytus; RICARDO S. BALARDIN, Universidade Federal de Santa Maria; WILSON S. VENANCIO, Universidade Estadual de Ponta Grossa. |
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Título: |
Eficiência de fungicidas para controle de mofo-branco (Sclerotinia sclerotiorum) em soja, na safra 2013/2014 - resultados sumarizados dos ensaios cooperativos. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Londrina, Embrapa Soja, 2015. |
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Páginas: |
4 p. |
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Série: |
(Embrapa Soja. Circular Técnica, 109). |
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ISSN: |
2176-2864 |
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Idioma: |
Português |
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Conteúdo: |
Com o objetivo de gerar resultados de pesquisa para auxiliar o registro e a recomendação de fungicidas para o controle dessa doença, os ensaios cooperativos de controle químico do mofo-branco em soja tiveram início na safra 2008/09. Essa rede de ensaios é coordenada pela Embrapa, Universidade de Rio Verde e Tagro, com apoio da ANDEF e empresas fabricantes de fungicidas, e os ensaios são conduzidos por pesquisadores de instituições de pesquisa e experimentação nos estados de Goiás, da Bahia, do Mato Grosso do Sul, do Mato Grosso, de Minas Gerais, de São Paulo, do Paraná e de Santa Catarina. Este trabalho apresenta os resultados sumarizados dos ensaios cooperativos, realizados na safra 2013/14. |
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Thesagro: |
Doença de planta; Fungicida; Fungo; Podridão da haste; Sclerotinia Sclerotiorum; Soja. |
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Thesaurus NAL: |
Fungicides; Plant diseases and disorders; Soybeans. |
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Categoria do assunto: |
--
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/122276/1/CT109-OL.pdf
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Marc: |
LEADER 02007nam a2200445 a 4500
001 2011247
005 2016-03-14
008 2015 bl uuuu u0uu1 u #d
022 $a2176-2864
100 1 $aMEYER, M. C.
245 $aEficiência de fungicidas para controle de mofo-branco (Sclerotinia sclerotiorum) em soja, na safra 2013/2014 - resultados sumarizados dos ensaios cooperativos.
260 $aLondrina, Embrapa Soja$c2015
300 $a4 p.
490 $a(Embrapa Soja. Circular Técnica, 109).
520 $aCom o objetivo de gerar resultados de pesquisa para auxiliar o registro e a recomendação de fungicidas para o controle dessa doença, os ensaios cooperativos de controle químico do mofo-branco em soja tiveram início na safra 2008/09. Essa rede de ensaios é coordenada pela Embrapa, Universidade de Rio Verde e Tagro, com apoio da ANDEF e empresas fabricantes de fungicidas, e os ensaios são conduzidos por pesquisadores de instituições de pesquisa e experimentação nos estados de Goiás, da Bahia, do Mato Grosso do Sul, do Mato Grosso, de Minas Gerais, de São Paulo, do Paraná e de Santa Catarina. Este trabalho apresenta os resultados sumarizados dos ensaios cooperativos, realizados na safra 2013/14.
650 $aFungicides
650 $aPlant diseases and disorders
650 $aSoybeans
650 $aDoença de planta
650 $aFungicida
650 $aFungo
650 $aPodridão da haste
650 $aSclerotinia Sclerotiorum
650 $aSoja
700 1 $aCAMPOS, H. D.
700 1 $aGODOY, C. V.
700 1 $aUTIAMADA, C. M.
700 1 $aMACHADO, A. Q.
700 1 $aPIMENTA, C. B.
700 1 $aCASSETARI NETO, D.
700 1 $aJACCOUD FILHO, D. S.
700 1 $aBORGES, E. P.
700 1 $aSIQUERI, F. V.
700 1 $aJULIATTI, F. C.
700 1 $aNUNES JUNIOR, J.
700 1 $aCARNEIRO, L. C.
700 1 $aSILVA, L. H. C. P. da
700 1 $aMADALOSSO, M.
700 1 $aBALARDIN, R. S.
700 1 $aVENANCIO, W. S.
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