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Registro Completo |
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Biblioteca(s): |
Embrapa Instrumentação. |
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Data corrente: |
05/07/2023 |
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Data da última atualização: |
27/11/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
TEODORO, K. B. R.; ALVARENGA, A. D.; OLIVEIRA, L. F. R.; CHAGAS, P. A. M.; LOPES. R. G.; ANDRE, R. S.; MERCANTE, L. A.; ALVES, F.; STRINGASCI, M. D.; BUZZA, H. H.; INADA, N. M.; CORREA, D. S. |
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Afiliação: |
KELCILENE BRUNA RICARDO TEODORO, UNIVERSIDADE FEDERAL DE SÃO CARLOS; AUGUSTO D. ALVARENGA; LUÍS FERNANDO ROCHA OLIVEIRA, UNIVERSIDADE FEDERAL DE SÃO CARLOS; PAULO AUGUSTO MARQUES CHAGAS, UNIVERSIDADE FEDERAL DE SÃO CARLOS (UFSCAR); RAPHAEL GUIMARÃES LOPES, UNIVERSIDADE FEDERAL DO TRIÂNGULO MINEIRO; RAFAELA DA SILVEIRA ANDRE, UNIVERSIDADE FEDERAL DE SÃO CARLOS; LUIZA AMIM MERCANTE, UNIVERSIDADE FEDERAL DA BAHIA (UFBA); FERNANDA ALVES; MIRIAN DENISE STRINGASCI, UNIVERSIDADE ESTADUAL DE CAMPINAS; HILDE HARB BUZZA, UNIVERSIDADE DE SÃO PAULO (USP); NATALIA MAYUMI INADA, UNIVERSIDADE DE SÃO PAULO (USP); DANIEL SOUZA CORREA, CNPDIA. |
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Título: |
Fast Fabrication of Multifunctional PCL/Curcumin Nanofibrous Membranes for Wound Dressings. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
ACS Applied Bio Materials, v. 6, 2023. |
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Páginas: |
2325–2337 |
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DOI: |
https://doi.org/10.1021/acsabm.3c00177 |
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Idioma: |
Inglês |
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Conteúdo: |
herapeutic intervention to skin wounds requires covering the affected area with wound dressings. Interdisciplinary efforts have focused on the development of smart bandages that can perform multiple functions. In this direction, here, we designed a low cost (U$0.012 per cm2 ) multifunctional therapeutic wound dressing fabricated by loading curcumin (CC) into poly(ϵ-caprolactone) (PCL) nanofibers using solution blow spinning (SBS). The freestanding PCL/CC bandages were characterized by distinct physicochemical approaches and were successful in performing varied functions, including controlled release of CC, colorimetric indication of the wound conditions, barrier against microorganisms, being biocompatible, and providing a photosensitive platform for antimicrobial photodynamic therapy (aPDT). The chemical nature of PCL and CC and the interactions between these components allowed CC to be released for 192 h (ca. 8 days), which could be correlated with the Korsmeyer−Peppas model, with a burst release suitable to treat the inflammatory phase. Due to the CC keto−enol tautomerism, an optical indication of the healing status could be obtained using PCL/CC, which occurred immediately, ranging between red/orange and yellow shades. The effect against pathogenic microorganisms evaluated by agar disc-diffusion, affected skin wound simulation (ex vivo), and microbial penetration tests demonstrated the ability to block and inhibit microbial permeation in different environments. The biocompatibilities of PCL and PCL/CC were verified by in vitro cytotoxicity study, which demonstrated that cell viabilities average above 94 and 96% for human dermal fibroblasts. In addition, the proposed bandage responded to aPDT applied to an in vivo assay, showing that, when irritated, PCL/CC was able to reduce the bacteria present on the real wound of mice. In summary, our findings demonstrate that using PCL and CC to produce nonwovens by the SBS technique offers potential for the rapid fabrication of biocompatible and multifunctional wound dressings, paving the way for large-scale production and utilization of such dressings in the treatment of skin wounds. Menosherapeutic intervention to skin wounds requires covering the affected area with wound dressings. Interdisciplinary efforts have focused on the development of smart bandages that can perform multiple functions. In this direction, here, we designed a low cost (U$0.012 per cm2 ) multifunctional therapeutic wound dressing fabricated by loading curcumin (CC) into poly(ϵ-caprolactone) (PCL) nanofibers using solution blow spinning (SBS). The freestanding PCL/CC bandages were characterized by distinct physicochemical approaches and were successful in performing varied functions, including controlled release of CC, colorimetric indication of the wound conditions, barrier against microorganisms, being biocompatible, and providing a photosensitive platform for antimicrobial photodynamic therapy (aPDT). The chemical nature of PCL and CC and the interactions between these components allowed CC to be released for 192 h (ca. 8 days), which could be correlated with the Korsmeyer−Peppas model, with a burst release suitable to treat the inflammatory phase. Due to the CC keto−enol tautomerism, an optical indication of the healing status could be obtained using PCL/CC, which occurred immediately, ranging between red/orange and yellow shades. The effect against pathogenic microorganisms evaluated by agar disc-diffusion, affected skin wound simulation (ex vivo), and microbial penetration tests demonstrated the ability to block and inhibit microbial permeation in different enviro... Mostrar Tudo |
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Palavras-Chave: |
Natural dye; Optical sensor; Photodynamic therapy; Solution blow spinning; Wound dressing. |
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Categoria do assunto: |
-- |
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Marc: |
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Registro original: |
Embrapa Instrumentação (CNPDIA) |
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