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 | Acesso ao texto completo restrito à biblioteca da Embrapa Instrumentação. Para informações adicionais entre em contato com cnpdia.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Instrumentação. |
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Data corrente: |
20/04/2023 |
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Data da última atualização: |
28/11/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CATUNDA, L. G. S.; PRADO, T. M. do; OLIVEIRA, T. R. de; SANTOS, D. J. A. dos; GOMES, N. O.; CORREA, D. S.; FARIA, R. C.; MACHADO, S. A. S. |
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Afiliação: |
LUCAS GOMES DA SILVA CATUNDA, UNIVERSITY OF SAO PAULO; THIAGO MARTIMIANO DO PRADO, UNIVERSITY OF SAO PAULO; TASSIA REGINA DE OLIVEIRA, FEDERAL UNIVERSITY OF SAO CARLOS; DANIEL JÚNIOR ALMEIDA DOS SANTOS, FEDERAL UNIVERSITY OF SAO CARLOS; NATHALIA OEZAU GOMES, UNIVERSITY OF SAO PAULO; DANIEL SOUZA CORREA, CNPDIA; RONALDO CENSI FARIA, FEDERAL UNIVERSITY OF SAO CARLOS; SÉRGIO ANTONIO SPINOLA MACHADO, UNIVERSITY OF SAO PAULO. |
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Título: |
SARS-CoV-2 detection enabled by a portable and label-free photoelectrochemical genosensor using graphitic carbon nitride and gold nanoparticles. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Electrochimica Acta, v. 451, 142271, 2023. |
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Páginas: |
10 p. |
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ISSN: |
0013-4686 |
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DOI: |
https://doi.org/10.1016/j.electacta.2023.142271 |
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Idioma: |
Inglês |
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Conteúdo: |
Fast, sensitive, simple, and cheap sensors are highly desirable to be applied in the health system because they improve point-of-care diagnostics, which can reduce the number of cases of infection or even deaths. In this context, here we report the development of a label-free genosensor using a screen-printed electrode modified with 2D-carbonylated graphitic carbon nitride (c-g-C3N4), poly(diallyldimethylammonium) chloride (PDDA), and glutathione-protected gold nanoparticles (GSH-AuNPs) for photoelectrochemical (PEC) detection of SARS-CoV-2. We also made use of Arduino and 3D printing to miniaturize the sensor device. The electrode surface was characterized by AFM and SEM techniques, and the gold nanoparticles by UV?Vis spectrophotometry. For SARSCoV-2 detection, capture probe DNA was immobilized on the electrode surface. The hybridization of the final genosensor was tested with a synthetic single-strand DNA target and with natural saliva samples using the photoelectrochemistry method. The device presented a linear range from 1 to 10,000 fmol L− 1 and a limit of detection of 2.2 and 3.4 fmol L− 1 using cpDNA 1A and 3A respectively. The sensibility and accuracy found for the genosensor using cpDNA 1A using biological samples were 93.3 and 80% respectively, indicating the potential of the label-free and portable genosensor to detect SARS-CoV-2 RNA in saliva samples. |
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Palavras-Chave: |
Arduino; Saliva test; Saliva test Miniaturized platform; SARS-CoV-2 detection; SARS-CoV-2 detection Photoelectrochemical (PEC) sensor. |
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Categoria do assunto: |
-- |
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Marc: |
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001 2153291
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022 $a0013-4686
024 7 $ahttps://doi.org/10.1016/j.electacta.2023.142271$2DOI
100 1 $aCATUNDA, L. G. S.
245 $aSARS-CoV-2 detection enabled by a portable and label-free photoelectrochemical genosensor using graphitic carbon nitride and gold nanoparticles.$h[electronic resource]
260 $c2023
300 $a10 p.
520 $aFast, sensitive, simple, and cheap sensors are highly desirable to be applied in the health system because they improve point-of-care diagnostics, which can reduce the number of cases of infection or even deaths. In this context, here we report the development of a label-free genosensor using a screen-printed electrode modified with 2D-carbonylated graphitic carbon nitride (c-g-C3N4), poly(diallyldimethylammonium) chloride (PDDA), and glutathione-protected gold nanoparticles (GSH-AuNPs) for photoelectrochemical (PEC) detection of SARS-CoV-2. We also made use of Arduino and 3D printing to miniaturize the sensor device. The electrode surface was characterized by AFM and SEM techniques, and the gold nanoparticles by UV?Vis spectrophotometry. For SARSCoV-2 detection, capture probe DNA was immobilized on the electrode surface. The hybridization of the final genosensor was tested with a synthetic single-strand DNA target and with natural saliva samples using the photoelectrochemistry method. The device presented a linear range from 1 to 10,000 fmol L− 1 and a limit of detection of 2.2 and 3.4 fmol L− 1 using cpDNA 1A and 3A respectively. The sensibility and accuracy found for the genosensor using cpDNA 1A using biological samples were 93.3 and 80% respectively, indicating the potential of the label-free and portable genosensor to detect SARS-CoV-2 RNA in saliva samples.
653 $aArduino
653 $aSaliva test
653 $aSaliva test Miniaturized platform
653 $aSARS-CoV-2 detection
653 $aSARS-CoV-2 detection Photoelectrochemical (PEC) sensor
700 1 $aPRADO, T. M. do
700 1 $aOLIVEIRA, T. R. de
700 1 $aSANTOS, D. J. A. dos
700 1 $aGOMES, N. O.
700 1 $aCORREA, D. S.
700 1 $aFARIA, R. C.
700 1 $aMACHADO, S. A. S.
773 $tElectrochimica Acta$gv. 451, 142271, 2023.
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