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 | Acesso ao texto completo restrito à biblioteca da Embrapa Café. Para informações adicionais entre em contato com biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Café. |
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Data corrente: |
27/03/2023 |
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Data da última atualização: |
12/04/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ANGELO, P. C. da S.; PEREIRA, L. F. P.; SERA, G. H.; SETA, T. |
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Afiliação: |
PAULA CRISTINA DA SILVA ANGELO, CNPCa; L. F. P. PEREIRA, IDR - PARANÁ; G. H. SERA, IDR - PARANÁ; T. SERA, IDR - PARANÁ. |
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Título: |
Coffee microspore cultivation to attain doubled-haploid plantlets. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Acta Horticulturae, v. 31, n. 1359, p. 123-130, 2023. |
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DOI: |
10.17660/ActaHortic.2023.1359.14 |
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Idioma: |
Inglês |
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Notas: |
Apresentado no INTERNATIONAL HORTICULTURAL CONGRESS, 31., 2022, Angers, França. |
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Conteúdo: |
Introgression of disease resistance from related Coffea species to C. arabica has been done by inter-specific hybridization. C. arabica (tetraploid) × C. racemosa (diploid) hybrids were obtained at the IDR-Paraná, Brazil. Some of the hybrids display impaired self-pollination, despite backcrossed to C. arabica. As an attempt to regenerate stable doubled-haploid plants, anthers and isolated microspores have been cultivated in vitro. It was considered that chromosomes captured in a microspore following meiosis could acquire homo and/or homeologous stability faster in vitro than in vivo. A C. arabica progeny was taken as control. Flowers were collected at the IDR campus in Londrina, when young microspores were uninucleate, and treated with 8% active chlorine. Microspores were extracted in 90 mM mannitol using a food mixer set to function for a few seconds, washed and centrifuged at 100× g twice, and cultivated in modified N6 liquid medium (105 cells mL-1 in 35 mm diameter plates). Anthers were excised using scalpels and cultivated in solid medium. Explants were kept for six months of continuous cultivation on that induction medium containing 6.5 mg L-1 auxins, 1.0 mg L-1 cytokinins and 0.5 mg L-1 gibberellin (GA3), at 27°C under dark. Microspores produced embryo-like structures or microcalli in very low frequencies (0.3 per plate). Anthers, on the other hand, produced embryogenic calli. Colonization by an invariant morphotype of fungus took away 70% of Arabica coffee control anthers and 5% of the asseptic anthers produced friable calli, some embryogenic but arrested when compared with calli produced by the inter-specific hybrid anthers. Colonization by an invariant morphotype of bacteria took away 25% of hybrid anthers and 8% of produced embryogenic tissue, with globular embryos simultaneously multiplying and maturing upon transfer to N6 medium. Embryo conversion and photomorphogenesis under light on auxin/cytokinin ratio = 2 plus GA3 are going on, 1.2 year after inoculation. MenosIntrogression of disease resistance from related Coffea species to C. arabica has been done by inter-specific hybridization. C. arabica (tetraploid) × C. racemosa (diploid) hybrids were obtained at the IDR-Paraná, Brazil. Some of the hybrids display impaired self-pollination, despite backcrossed to C. arabica. As an attempt to regenerate stable doubled-haploid plants, anthers and isolated microspores have been cultivated in vitro. It was considered that chromosomes captured in a microspore following meiosis could acquire homo and/or homeologous stability faster in vitro than in vivo. A C. arabica progeny was taken as control. Flowers were collected at the IDR campus in Londrina, when young microspores were uninucleate, and treated with 8% active chlorine. Microspores were extracted in 90 mM mannitol using a food mixer set to function for a few seconds, washed and centrifuged at 100× g twice, and cultivated in modified N6 liquid medium (105 cells mL-1 in 35 mm diameter plates). Anthers were excised using scalpels and cultivated in solid medium. Explants were kept for six months of continuous cultivation on that induction medium containing 6.5 mg L-1 auxins, 1.0 mg L-1 cytokinins and 0.5 mg L-1 gibberellin (GA3), at 27°C under dark. Microspores produced embryo-like structures or microcalli in very low frequencies (0.3 per plate). Anthers, on the other hand, produced embryogenic calli. Colonization by an invariant morphotype of fungus took away 70% of Arabica coffee control ant... Mostrar Tudo |
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Thesagro: |
Embriogénese; Pólen; Rubiaceae. |
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Thesaurus Nal: |
Embryogenesis; Pollen. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02752naa a2200241 a 4500
001 2152776
005 2023-04-12
008 2023 bl uuuu u00u1 u #d
024 7 $a10.17660/ActaHortic.2023.1359.14$2DOI
100 1 $aANGELO, P. C. da S.
245 $aCoffee microspore cultivation to attain doubled-haploid plantlets.$h[electronic resource]
260 $c2023
500 $aApresentado no INTERNATIONAL HORTICULTURAL CONGRESS, 31., 2022, Angers, França.
520 $aIntrogression of disease resistance from related Coffea species to C. arabica has been done by inter-specific hybridization. C. arabica (tetraploid) × C. racemosa (diploid) hybrids were obtained at the IDR-Paraná, Brazil. Some of the hybrids display impaired self-pollination, despite backcrossed to C. arabica. As an attempt to regenerate stable doubled-haploid plants, anthers and isolated microspores have been cultivated in vitro. It was considered that chromosomes captured in a microspore following meiosis could acquire homo and/or homeologous stability faster in vitro than in vivo. A C. arabica progeny was taken as control. Flowers were collected at the IDR campus in Londrina, when young microspores were uninucleate, and treated with 8% active chlorine. Microspores were extracted in 90 mM mannitol using a food mixer set to function for a few seconds, washed and centrifuged at 100× g twice, and cultivated in modified N6 liquid medium (105 cells mL-1 in 35 mm diameter plates). Anthers were excised using scalpels and cultivated in solid medium. Explants were kept for six months of continuous cultivation on that induction medium containing 6.5 mg L-1 auxins, 1.0 mg L-1 cytokinins and 0.5 mg L-1 gibberellin (GA3), at 27°C under dark. Microspores produced embryo-like structures or microcalli in very low frequencies (0.3 per plate). Anthers, on the other hand, produced embryogenic calli. Colonization by an invariant morphotype of fungus took away 70% of Arabica coffee control anthers and 5% of the asseptic anthers produced friable calli, some embryogenic but arrested when compared with calli produced by the inter-specific hybrid anthers. Colonization by an invariant morphotype of bacteria took away 25% of hybrid anthers and 8% of produced embryogenic tissue, with globular embryos simultaneously multiplying and maturing upon transfer to N6 medium. Embryo conversion and photomorphogenesis under light on auxin/cytokinin ratio = 2 plus GA3 are going on, 1.2 year after inoculation.
650 $aEmbryogenesis
650 $aPollen
650 $aEmbriogénese
650 $aPólen
650 $aRubiaceae
700 1 $aPEREIRA, L. F. P.
700 1 $aSERA, G. H.
700 1 $aSETA, T.
773 $tActa Horticulturae$gv. 31, n. 1359, p. 123-130, 2023.
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Registro original: |
Embrapa Café (CNPCa) |
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| Registros recuperados : 22 | |
| 6. |  | ASSIS, O. B. G.; MELLO, M.; FERRANTE, M. SEM and EDS characterization of microstructural changes caused by Cr and Co addition in NdFeB magnetics. In: INTERNATIONAL CONGRESS ON ELECTRON MICROSCOPY=MEMORIAS DEL CONGRESO INTERNACIONAL DE MICROSCOPIA ELETRÓNICA-ICEM, 14., 1998, Cancún. Electron microscopy 1998: paper presented... Bristol: Institute of Physis Publshing, 1998, v.2, p. 527-528. Edited by: Héctor A. Calderón Benavides, Miguel José Yacamán.| Biblioteca(s): Embrapa Instrumentação. |
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| 8. | | PINTO, D. dos S. C.; GOUVEIA, L. P.; FLORES, S. A.; NOBRE, M. R.; BRANDAO, H. de M.; FERRANTE, M. Determination of doramectin protocols using computational modeling for the treat ment of Rhipicephalus microplus in cattle. In: CONFERENCE OF THE WORLD ASSOCIATION FOR THE ADVANCEMENTS OF VETERINARY PARASITOLOGY, 30., 2025, Curitiba. Abstract book. Curitiba: Universidade Federal do Paraná, 2025. p. 357-358.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 9. | | SOUSA, N. P.; LIMA, A. M. C.; ARAÚJO, J. B. de; ANDRADE, I. M.; FERRANTE, M.; FACCIOLI-MARTINS, P. Y. Determinação do ponto de corte epidemiológico, curva de morte e estimativa do índice farmacocinético/farmacodinâmico da cloxacilina em Corynebacterium pseudotuberculosis. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA CAPRINOS E OVINOS, 11., 2022, Sobral. Anais... Sobral: Embrapa Caprinos e Ovinos, 2023. p. 39-40. (Embrapa Caprinos e Ovinos. Eventos técnicos & científicos).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 10. | | BELLETTINI, S.; MIEKO, N.; BELLETTINI, T.; NISHIMURA, M.; J. FERRANTE, M. J.; SILVA, G. T. G. da; GONÇALVES, P. M. Inseticidas em tratamento de sementes no controle do Tripes Frankliniella schultzei (Trybom, 1920) no algodoeiro. In: CONGRESSO BRASILEIRO DO ALGODÃO, 6., 2007, Uberlândia. Resumos... Uberlândia, 2007. p. 1-3 1 CD-ROM| Biblioteca(s): Embrapa Algodão. |
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| 11. | | ASSIS, O. B. G.; CRNKOVIC, F. C.; MACHADO, R.; AVACA, L. A.; FERRANTE, M.; MACHADO, S. A. S. Powder metallurgy processing of Ni-Co base electrodes for application in water electrolysis. In: INTERNATIONAL LATIN-AMERICAN CONFERENCE ON POWDER TECHNOLOGY=CONFERENCIA INTERNACIONAL LATINO-AMERICANA EM TECNOLOGIA DO PO, 1., Nov. 1997, Aguas de Lindoia, SP. Information/Informativo... Aguas de Lindoia: Associacao Brasileira de Ceramica, 1997. não paginado.| Biblioteca(s): Embrapa Instrumentação. |
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| 12. | | ASSIS, O. B. G.; CRNKOVIC, F. C.; MACHADO, R.; AVACA, L. A.; FERRANTE, M.; MACHADO, S. A. S. Powder metallurgy processing of Ni-Co base electrodes for application in water electrolysis. Materials Sciences Forum, Aemannsdorf, v.299-300, p. 371-375, 1999.| Biblioteca(s): Embrapa Instrumentação. |
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| 13. | | RODRIGUES, M. L. C.; LIMA, A. M. C.; COSTA, V. M. D.; FELIX, L. A.; FERRANTE, M.; FACCIOLI-MARTINS, P. Y. Dinâmica de morte e ponto de corte epidemiológico de cepas de Corynebacterium pseudotuberculosis tratadas com cloxacilina. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA CAPRINOS E OVINOS, 12., 2023, Sobral. Resumos... Sobral: Embrapa Caprinos e Ovinos, 2024. p. 13. (Embrapa Caprinos e Ovinos. Eventos técnicos & científicos, 3).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 14. | | NOBRE, M. R.; PINTO, D. dos S. C.; LOURES, M. D. de A.; LÉPORI, C.; FERRANTE, M.; BRANDAO, H. de M. Development of a nanocarrier pharmaceutical formulation containing metronidazole for the topical treatment of bovine trichomoniasis. In: CONFERENCE OF THE WORLD ASSOCIATION FOR THE ADVANCEMENTS OF VETERINARY PARASITOLOGY, 30., 2025, Curitiba. Abstract book. Curitiba: Universidade Federal do Paraná, 2025. p. 357.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 15. | | TAMEIRÃO, E. R.; FARIA, P. H. A. de; GONZAGA, L. W. F.; ALMEIDA, J. V. F. C. de; SOUSA, T. M.; BRANDAO, H. de M.; FERRANTE, M. Modelagem farmacocinética/farmacodinâmica do florfenicol para o tratamento da adenite equina por meio da simulação de Monte Carlo. Revista Brasileira de Ciência Veterinária, v. 28, n. 2, p. 75-80, 2021.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 3 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 16. | | TAMEIRÃO, E. R.; RUBIM, F. M.; FELIX, L. A.; GONZAGA, L. W. F.; BRANDAO, H. de M.; MURGAS, L. D.; FERRANTE, M. Modelo farmacocinético de florfenicol en tilapias (Oreochromis niloticus) sometidas a diferentes temperaturas de crianza. Revista de Investigaciones Veterinarias del Perú, v. 33, n. 6, e22433, 2022.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 17. | | FELIX, L. A.; GONZAGA, L. W. F.; SOARES, B. C. F.; SUÁREZ, G.; BRANDAO, H. de M.; FACCIOLI-MARTINS, P. Y.; FERRANTE, M. PK/PD integration for intramuscular dose determination of intramuscular sodium cloxacillin for infections caused by Staphylococcus spp in goat. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, v. 75, n. 6, p. 1087-1096, 2023.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Caprinos e Ovinos; Embrapa Gado de Leite. |
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| 19. | | NOBRE, M. R.; LOURES, M. D. de A.; LÉPORI, C.; FERRANTE, M.; GLANZMANN, D.; MARQUES, R.; CÓCERES, V. M.; BRANDAO, H. de M. Avaliação do nanoencapsulamento do metronidazol: resultados preliminares. In: ENCONTRO NACIONAL DA REDE DE PESQUISA E INOVAÇÃO EM SANIDADE E PECUÁRIA LEITEIRA, 2., 2024, Lavras. Mastite, qualidade do leite e queijo minas artesanal: anais do evento. Lavras: Universidade Federal de Lavras, 2024. p. 25.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| 20. | | GUIMARAES, I. B.; ALMEIDA, J. V. F. C.; VIANELLO, J.; BATALHA, B. G.; FERRANTE, M.; LOURES, M. D. de A.; GERN, J. C.; MARTINS, P. Y. F.; GUIMARÃES, A. S.; BRANDAO, H. de M. Farmacocinética de nanopartículas de cloxacilina administradas em caprinos diagnosticados com linfadenite caseosa. In: WORKSHOP DE INICIAÇÃO CIENTÍFICA DA EMBRAPA GADO DE LEITE, 24., 2019, Juiz de Fora. Anais... Juiz de Fora: Embrapa Gado de Leite, 2019. 3 p.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Gado de Leite. |
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| Registros recuperados : 22 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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