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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
22/03/2023 |
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Data da última atualização: |
23/03/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SOUSA, A. L. M. de; PINHEIRO, R. R.; ARAUJO, J. F.; PEIXOTO, R. M.; AZEVEDO, D. A. A. de; LIMA, A. M. C.; CANUTO, K. M.; RIBEIRO, P. R. V.; SOUZA, A. S. de Q.; SOUZA, S. C. R.; AMORIM, S. L. de; AMARAL, G. P.; SOUZA, V. de; MORAIS, S. M. de; ANDRIOLI, A.; TEIXEIRA, M. F. da S. |
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Afiliação: |
ANA LIDIA MADEIRA DE SOUSA, Laboratory of Virology (LABOVIR), State University of Ceará (UECE); RAYMUNDO RIZALDO PINHEIRO, CNPC; JUSCILANIA FURTADO ARAUJO, University Center INTA (UNINTA), Tianguá, CE, Brazil; RENATO MESQUITA PEIXOTO, Vale do Salgado University Center (UNIVS) - Icó, CE, Brazil; Terra Nordeste College (FATENE) - Caucaia, CE, Brazil; DALVA ALANA ARAGAO DE AZEVEDO; ANA MILENA CESAR LIMA, National Council for Scientific and Technological Development (DCR-CNPq/FUNCAP), Embrapa; KIRLEY MARQUES CANUTO, CNPAT; PAULO RICELI VASCONCELOS RIBEIRO, CNPAT; ANA SHEILA DE QUEIROZ SOUZA; SAMARA CRISTINA ROCHA SOUZA; SARA LUCENA DE AMORIM; GABRIEL PAULA AMARAL, State University of Acaraú Valley - Sobral, CE, Brazil; VIVIANE DE SOUZA, CNPC; SELENE MAIA DE MORAIS, Laboratory of Chemistry and Natural Products (LQPN), Ceará State University, Fortaleza, CE, Brazil; ALICE ANDRIOLI PINHEIRO, CNPC; MARIA FATIMA DA SILVA TEIXEIRA, Laboratory of Virology (LABOVIR), State University of Ceará (UECE), Fortaleza, CE, Brazil. |
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Título: |
In vitro antiviral efect of ethanolic extracts from Azadirachta indica and Melia azedarach against goat lentivirus in colostrum and milk. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Scientific Reports, v. 13, article 4677, Mar. 2023. |
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DOI: |
https://doi.org/10.1038/s41598-023-31455-5 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: This study aimed to evaluate, in vitro, the use of leaf extracts of Azadirachta indica (A. indica) and Melia azedarach (M. azedarach) as antivirals against caprine lentivirus (CLV) in colostrum and milk of goat nannies. These were collected from eight individuals and infected with the standard strain of CLV. Samples were then subdivided into aliquots and treated with 150 µg/mL of crude extract, and with ethyl acetate and methanol fractions for 30, 60, and 90 min. Next, somatic cells from colostrum and milk were co-cultured with cells from the ovine third eyelid. After this step, viral titers of the supernatants collected from treatments with greater efficacy in co-culture were assessed. The organic ethyl acetate fractions of both plants at 90 min possibly inhibited the viral activity of CLV by up to a thousandfold in colostrum. In milk, this inhibition was up to 800 times for the respective Meliaceae. In conclusion, the ethanolic fraction of ethyl acetate from both plants demonstrated efficacy against CLV in samples from colostrum and milk when subjected to treatment, which was more effective in colostrum. |
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Palavras-Chave: |
Antiviral plants; CAEV; Caprine Lentivirus; CLV; Composto bioativo; Ethanolic extract; Extrato de plantas; SRLV. |
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Thesagro: |
Azadirachta Indica; Extrato Vegetal; Fitoterapia; Melia Azedarach; Nim; Planta Medicinal. |
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Thesaurus Nal: |
Antiviral properties; Goat diseases; Medicinal plants; Phytotherapy; Plant extracts; Sheep diseases. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1152602/1/CNPC-2023-In-vitro-antiviral-efect-of.pdf
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Marc: |
LEADER 02726naa a2200553 a 4500
001 2152602
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008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1038/s41598-023-31455-5$2DOI
100 1 $aSOUSA, A. L. M. de
245 $aIn vitro antiviral efect of ethanolic extracts from Azadirachta indica and Melia azedarach against goat lentivirus in colostrum and milk.$h[electronic resource]
260 $c2023
520 $aAbstract: This study aimed to evaluate, in vitro, the use of leaf extracts of Azadirachta indica (A. indica) and Melia azedarach (M. azedarach) as antivirals against caprine lentivirus (CLV) in colostrum and milk of goat nannies. These were collected from eight individuals and infected with the standard strain of CLV. Samples were then subdivided into aliquots and treated with 150 µg/mL of crude extract, and with ethyl acetate and methanol fractions for 30, 60, and 90 min. Next, somatic cells from colostrum and milk were co-cultured with cells from the ovine third eyelid. After this step, viral titers of the supernatants collected from treatments with greater efficacy in co-culture were assessed. The organic ethyl acetate fractions of both plants at 90 min possibly inhibited the viral activity of CLV by up to a thousandfold in colostrum. In milk, this inhibition was up to 800 times for the respective Meliaceae. In conclusion, the ethanolic fraction of ethyl acetate from both plants demonstrated efficacy against CLV in samples from colostrum and milk when subjected to treatment, which was more effective in colostrum.
650 $aAntiviral properties
650 $aGoat diseases
650 $aMedicinal plants
650 $aPhytotherapy
650 $aPlant extracts
650 $aSheep diseases
650 $aAzadirachta Indica
650 $aExtrato Vegetal
650 $aFitoterapia
650 $aMelia Azedarach
650 $aNim
650 $aPlanta Medicinal
653 $aAntiviral plants
653 $aCAEV
653 $aCaprine Lentivirus
653 $aCLV
653 $aComposto bioativo
653 $aEthanolic extract
653 $aExtrato de plantas
653 $aSRLV
700 1 $aPINHEIRO, R. R.
700 1 $aARAUJO, J. F.
700 1 $aPEIXOTO, R. M.
700 1 $aAZEVEDO, D. A. A. de
700 1 $aLIMA, A. M. C.
700 1 $aCANUTO, K. M.
700 1 $aRIBEIRO, P. R. V.
700 1 $aSOUZA, A. S. de Q.
700 1 $aSOUZA, S. C. R.
700 1 $aAMORIM, S. L. de
700 1 $aAMARAL, G. P.
700 1 $aSOUZA, V. de
700 1 $aMORAIS, S. M. de
700 1 $aANDRIOLI, A.
700 1 $aTEIXEIRA, M. F. da S.
773 $tScientific Reports$gv. 13, article 4677, Mar. 2023.
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Embrapa Caprinos e Ovinos (CNPC) |
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| 1. |  | SOUSA, A. L. M. de; PINHEIRO, R. R.; ARAUJO, J. F.; PEIXOTO, R. M.; AZEVEDO, D. A. A. de; LIMA, A. M. C.; CANUTO, K. M.; RIBEIRO, P. R. V.; SOUZA, A. S. de Q.; SOUZA, S. C. R.; AMORIM, S. L. de; AMARAL, G. P.; SOUZA, V. de; MORAIS, S. M. de; ANDRIOLI, A.; TEIXEIRA, M. F. da S. In vitro antiviral efect of ethanolic extracts from Azadirachta indica and Melia azedarach against goat lentivirus in colostrum and milk. Scientific Reports, v. 13, article 4677, Mar. 2023.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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