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Registro Completo |
Biblioteca(s): |
Embrapa Semiárido. |
Data corrente: |
17/02/2023 |
Data da última atualização: |
01/03/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
BASTOS, B. D. M.; SILVA, N. P. da S.; GONÇALVES, P. R.; CANDIDO, A. E. C. M.; BARBERINO, R. de S.; MONTE, A. P. O. do; NOGUEIRA, D. M.; CORDEIRO, M. F.; MATOS, M. H. T. de; LOPES JUNIOR, E. S. |
Afiliação: |
BRUNA DIAS MANGUEIRA BASTOS, UNIVASF; MARIA NAIARA PEREIRA DA SILVA, UNIVASF; PRICILA RAMOS GONÇALVES, UNIVASF; ANA ELISA CUSTÓDIO MONTES CÂNDIDO, UNIVASF; RICÁSSIO DE SOUSA BARBERINO, UNIVASF; ALANE PAINS OLIVEIRA DO MONTE, UNIVASF; DANIEL MAIA NOGUEIRA, CPATSA; MABEL FREITAS CORDEIRO, UNIVASF; MARIA HELENA TAVARES DE MATOS, UNIVASF; EDILSON SOARES LOPES JÚNIOR, UNIVASF. |
Título: |
Effect of different gonadotropins on in vitro maturation of sheep oocytes. |
Ano de publicação: |
2022 |
Fonte/Imprenta: |
Semina: Ciências Agrárias, v. 43, N. 6, p. 2731-2742, nov./dez. 2022. |
ISSN: |
1676-546X |
DOI: |
https://doi.org/10.5433/1679-0359.2022v43n6p2731 |
Idioma: |
Inglês |
Conteúdo: |
The aim of this study was to examine the effect of replacing the use of follicle-stimulating hormone (FSH) with equine chorionic gonadotropin (eCG) and human chorionic gonadotropin (hCG) on the in vitro maturation (IVM) of sheep oocytes. After sheep ovaries were collected (n=300), the cumulus-oocyte complexes were aspirated, selected, and divided into four groups according to the IVM medium: CON group, in which the basic IVM medium was used; and eCG, hCG, and FSH groups, in which the oocytes were immersed in basic IVM medium with 10 IU/mL eCG, 10 IU/mL hCG, and 10 μg/mL FSH-p, respectively. In vitro maturation of the oocytes was performed at 38.5 °C, in a humidified atmosphere of 5% CO2 in air, for 24 h. Subsequently, the oocytes were evaluated for the degree of cumulus-cell expansion, chromatin configuration, GSH levels, and active mitochondria. There were no significant differences for the rate of cumulus cell expansion. The percentage of oocytes in MII was higher in the eCG group than in the CON and hCG groups (P<0.05) and similar to that of the FSH group. In conclusion, eCG can be used as a substitute for FSH in IVM of sheep oocytes. |
Palavras-Chave: |
GSH; Hoechst 33342; Hormônio folículo estimulante; Metafase II; Oócitos ovinos. |
Thesagro: |
Hormônio Animal; Maturação Artificial; Ovino. |
Thesaurus Nal: |
Follicle-stimulating hormone; Metaphase; Sheep. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1151836/1/Efeito-de-diferentes-gonadotrofinas-na-maturacao.-2022.pdf
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Marc: |
LEADER 02307naa a2200385 a 4500 001 2151836 005 2023-03-01 008 2022 bl uuuu u00u1 u #d 022 $a1676-546X 024 7 $ahttps://doi.org/10.5433/1679-0359.2022v43n6p2731$2DOI 100 1 $aBASTOS, B. D. M. 245 $aEffect of different gonadotropins on in vitro maturation of sheep oocytes.$h[electronic resource] 260 $c2022 520 $aThe aim of this study was to examine the effect of replacing the use of follicle-stimulating hormone (FSH) with equine chorionic gonadotropin (eCG) and human chorionic gonadotropin (hCG) on the in vitro maturation (IVM) of sheep oocytes. After sheep ovaries were collected (n=300), the cumulus-oocyte complexes were aspirated, selected, and divided into four groups according to the IVM medium: CON group, in which the basic IVM medium was used; and eCG, hCG, and FSH groups, in which the oocytes were immersed in basic IVM medium with 10 IU/mL eCG, 10 IU/mL hCG, and 10 μg/mL FSH-p, respectively. In vitro maturation of the oocytes was performed at 38.5 °C, in a humidified atmosphere of 5% CO2 in air, for 24 h. Subsequently, the oocytes were evaluated for the degree of cumulus-cell expansion, chromatin configuration, GSH levels, and active mitochondria. There were no significant differences for the rate of cumulus cell expansion. The percentage of oocytes in MII was higher in the eCG group than in the CON and hCG groups (P<0.05) and similar to that of the FSH group. In conclusion, eCG can be used as a substitute for FSH in IVM of sheep oocytes. 650 $aFollicle-stimulating hormone 650 $aMetaphase 650 $aSheep 650 $aHormônio Animal 650 $aMaturação Artificial 650 $aOvino 653 $aGSH 653 $aHoechst 33342 653 $aHormônio folículo estimulante 653 $aMetafase II 653 $aOócitos ovinos 700 1 $aSILVA, N. P. da S. 700 1 $aGONÇALVES, P. R. 700 1 $aCANDIDO, A. E. C. M. 700 1 $aBARBERINO, R. de S. 700 1 $aMONTE, A. P. O. do 700 1 $aNOGUEIRA, D. M. 700 1 $aCORDEIRO, M. F. 700 1 $aMATOS, M. H. T. de 700 1 $aLOPES JUNIOR, E. S. 773 $tSemina: Ciências Agrárias$gv. 43, N. 6, p. 2731-2742, nov./dez. 2022.
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Embrapa Semiárido (CPATSA) |
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1. | | BASTOS, B. D. M.; SILVA, N. P. da S.; GONÇALVES, P. R.; CANDIDO, A. E. C. M.; BARBERINO, R. de S.; MONTE, A. P. O. do; NOGUEIRA, D. M.; CORDEIRO, M. F.; MATOS, M. H. T. de; LOPES JUNIOR, E. S. Effect of different gonadotropins on in vitro maturation of sheep oocytes. Semina: Ciências Agrárias, v. 43, N. 6, p. 2731-2742, nov./dez. 2022.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
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