| |
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Hortaliças. Para informações adicionais entre em contato com biblioteca@embrapa.br. |
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Hortaliças. |
|
Data corrente: |
15/02/2023 |
|
Data da última atualização: |
15/02/2023 |
|
Tipo da produção científica: |
Nota Técnica/Nota Científica |
|
Autoria: |
BARBOSA, J. C.; ECKSTEIN, B.; INOUE-NAGATA, A. K.; BERGAMIN FILHO, A.; BEDENDO, I. P. |
|
Afiliação: |
JÚLIO CÉSAR BARBOSA, ESALQ / UNIVERSIDADE DE SÃO PAULO; BARBARA ECKSTEIN, Cenargen; ALICE KAZUKO INOUE NAGATA, CNPH; ARMANDO BERGAMIN FILHO, ESALQ / UNIVERSIDADE DE SÃO PAULO; IVAN PAULO BEDENDO, ESALQ / UNIVERSIDADE DE SÃO PAULO. |
|
Título: |
Molecular delineation of a phytoplasma representative of the novel 16SrVII-G subgroup found in citrus trees with huanglongbing symptoms. |
|
Ano de publicação: |
2022 |
|
Fonte/Imprenta: |
Journal of Plant Diseases and Protection, v. 129, p. 419-424, 2022. |
|
DOI: |
https://doi.org/10.1007/s41348-021-00532-0 |
|
Idioma: |
Inglês |
|
Conteúdo: |
Leaf symptoms of chlorosis and blotchy mottling, typically associated with agents of huanglongbing, were found in commercial citrus orchard located in the state of São Paulo, the main citrus producer region in Brazil. Affected trees tested negative for bacteria ?Ca. Liberibacter americanus? and ?Ca. Liberibacter asiaticus?. So, the total DNA was submitted to nested PCR employing the primer pairs P1/Tint followed by R16F2n/R16R2. Genomic fragments of 1.2 kb were amplified, sequenced and analyzed by computer-simulated RFLP, which confirmed the presence of a 16SrVII phytoplasma in symptomatic plants. Collective restriction patterns allowed distinguish the detected phytoplasma from all other members of the 16SrVII group using enzymes BfaI and MseI. In addition, the citrus phytoplasma showed a similarity coefficient lower than 0.97, when compared with all representative strains of subgroups within of the 16SrVII group, characterizing, therefore, a new subgroup. In agreement, the phylogenetic tree displayed that this strain emerges from a distinct branch in relation to those 16SrVII phytoplasmas currently described. Based on these features, the strain identified in the present study may be delineated as a new phytoplasma representative of the novel 16SrVII-G subgroup. Moreover, the results implicate citrus as a new host for a phytoplasma belonging to the group 16SrVII. |
|
Palavras-Chave: |
Chlorosis and blotchy mottling; Phloem bacteria; Phytopathogenic mollicutes; Wall-less bacteria. |
|
Thesagro: |
Fruta Cítrica. |
|
Thesaurus Nal: |
Candidatus Liberibacter americanus; Candidatus Liberibacter asiaticus. |
|
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
|
Marc: |
LEADER 02321naa a2200265 a 4500
001 2151789
005 2023-02-15
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s41348-021-00532-0$2DOI
100 1 $aBARBOSA, J. C.
245 $aMolecular delineation of a phytoplasma representative of the novel 16SrVII-G subgroup found in citrus trees with huanglongbing symptoms.$h[electronic resource]
260 $c2022
520 $aLeaf symptoms of chlorosis and blotchy mottling, typically associated with agents of huanglongbing, were found in commercial citrus orchard located in the state of São Paulo, the main citrus producer region in Brazil. Affected trees tested negative for bacteria ?Ca. Liberibacter americanus? and ?Ca. Liberibacter asiaticus?. So, the total DNA was submitted to nested PCR employing the primer pairs P1/Tint followed by R16F2n/R16R2. Genomic fragments of 1.2 kb were amplified, sequenced and analyzed by computer-simulated RFLP, which confirmed the presence of a 16SrVII phytoplasma in symptomatic plants. Collective restriction patterns allowed distinguish the detected phytoplasma from all other members of the 16SrVII group using enzymes BfaI and MseI. In addition, the citrus phytoplasma showed a similarity coefficient lower than 0.97, when compared with all representative strains of subgroups within of the 16SrVII group, characterizing, therefore, a new subgroup. In agreement, the phylogenetic tree displayed that this strain emerges from a distinct branch in relation to those 16SrVII phytoplasmas currently described. Based on these features, the strain identified in the present study may be delineated as a new phytoplasma representative of the novel 16SrVII-G subgroup. Moreover, the results implicate citrus as a new host for a phytoplasma belonging to the group 16SrVII.
650 $aCandidatus Liberibacter americanus
650 $aCandidatus Liberibacter asiaticus
650 $aFruta Cítrica
653 $aChlorosis and blotchy mottling
653 $aPhloem bacteria
653 $aPhytopathogenic mollicutes
653 $aWall-less bacteria
700 1 $aECKSTEIN, B.
700 1 $aINOUE-NAGATA, A. K.
700 1 $aBERGAMIN FILHO, A.
700 1 $aBEDENDO, I. P.
773 $tJournal of Plant Diseases and Protection$gv. 129, p. 419-424, 2022.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Hortaliças (CNPH) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
| Registros recuperados : 8 | |
| 1. |  | SANTOS, D. M.; CARVALHO, M. A. R.; FRÉZARD, F.; BEMQUERER, M. P.; PIMENTA, A. M. C.; DE LIMA, M. E. Activity and structure of the des-HIS16-LyeTx I, a new synthetic peptide with antimicrobial activity. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE BIOQUÍMICS E BIOLOGIA MOLECULAR - SBBq, 41., Foz do Iguaçú, Brasil. Resumos... Foz do Iguaçu: SBBq, 2012.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
|    |
| 2. | | PIMENTA, A. M. C.; RATES, B.; BLOCH JUNIOR, C.; GOMES, P. C.; SANTORO, M. M.; LIMA, M. E. de; RICHARDSON, M.; CORDEIRO, M. do N. Electrospray ionization quadrupole time-of-flight and matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometric analyses to solve micro heterogeneity in post-translationally modified peptides from Phoneutria nigriventer (Aranea, Ctenidae) venom. Rapid Communications in Mass Spectrometry, Chichester, UK, v. 19, p. 31-37, 2005.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
|  |
| 3. | | RATES, B.; SILVA, L. P. da; IRENO, I. C.; LEITE, F. S. F.; BORGES, M. H.; BLOCH JUNIOR, C.; LIMA, M. E. DE; PIMENTA, A. M. C. Peptidomic dissection of the skin secretion of Phasmahyla jandaia (Bokermann and Sazima, 1978) (Anura, Hylidae, Phyllomedusinae). Toxicon, v. 57, n. 1, p. 35-52, 2011.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
| |
| 4. | | RIFFEL, A.; S. FILHO, B. F.; OLIVEIRA, J. S.; RIBEIRO, T. F. L.; SANTOS, D. M.; PIMENTA, A. M. C.; SANTANA, A. E. G. The interaction between Diatraea saccharalis(Lapidoptera, Crambidae) and sugarcane: changes in plant's proteome. In: LATIN AMERICAN MEETING OF CHEMICAL ECOLOGY, 2., 2012, Huerta Grande. Abstracts... Huerta Grande: Latin American Association of Chemical Ecology, 2012. Resumo em anais.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
| |
| 5. | | RICHARDSON, M.; PIMENTA, A. M. C.; BEMQUERER, M. P.; SANTORO, M. M.; BEIRAO, P. S. L.; LIMA, M. E.; FIGUEIREDO, S. G.; BLOCH JUNIOR, C.; VASCONCELOS, E. A. R.; CAMPOS, F. A. P.; GOMES, P. C.; CORDEIRO, M. N. Comparison of the partial proteomes of the venoms of Brazilian spiders of the genus Phoneutria. Comparative Biochemistry and Physiology, Part C, v. 142, p. 173-187, 2006.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: -- - A |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
| |
| 6. | | LÚCIO, A. D.; CAMPOS, F. V.; RICHARDSON, M.; CORDEIRO, M. N.; MAZZONI, M. S. C.; LIMA, M. E. de; PIMENTA, A. M. C.; BEMQUERER, M. P.; FIGUEIREDO, S. G.; GOMES, P. C.; BEIRÃO, P. S. L. A new family of small (4kDa) neurotoxins from the venoms of spiders of the genus Phoneutria. Protein e Peptide Letters, v. 15, p.700-708, 2008.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: Internacional - A |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
| |
| 7. | | CISCOTTO, P. H. C.; RATES, B.; SILVA, D. A. F.; RICHARDSON, M.; SILVA, L. P. da; ANDRADE, H.; DONATO, M. F.; COTTA, G. A.; MARIA, W. S.; RODRIGUES, R. J.; SANCHEZ, E.; LIMA, M. E. de; PIMENTA, A. M. C. Venomic analysis and evaluation of antivenom cross-reactivity of South American Micrurus species. Journal of Proteomics, v. 74, p. 1810-1825, 2011.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 4 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
| |
| 8. | | SANTOS, D. M.; VERLY, R. M.; PILÓ VELOSO, D.; MARIA, M. de; CARVALHO, M. A. R. de; CISALPINO, P. S.; SOARES, B. M.; DINIZ, C. G.; FARIAS, L. M.; MOREIRA, D. F. F.; FRÉZARD, F.; BEMQUERER, M. P.; PIMENTA, A. M. C.; LIMA, M. E. de. LyeTx I, a potent antimicrobial peptide from the venom of the spider Lycosa erythrognatha. Amino Acids, v. 39, p. 135-144, 2010.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
| |
| Registros recuperados : 8 | |
|
| Expressão de busca inválida. Verifique!!! |
|
|
