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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
08/12/2022 |
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Data da última atualização: |
12/12/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CARVALHO, M. de J. da S. de; SOUZA, A. da S.; EMANUELA BARBOSA SANTOS; SOARES FILHO, W. dos S.; LEDO, C. A. da S.; COSTA, E. M. R.; SOUZA, F. V. D. |
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Afiliação: |
MARIANE DE JESUS DA SILVA DE CARVALHO, UNIVERSIDADE FEDERAL DO RECÔNCAVO DA BAHIA; ANTONIO DA SILVA SOUZA, CNPMF; EMANUELA BARBOSA SANTOS, UNIVERSIDADE FEDERAL DO CEARÁ; WALTER DOS SANTOS SOARES FILHO, CNPMF; CARLOS ALBERTO DA SILVA LEDO, CNPMF; EVA MARIA RODRIGUES COSTA; FERNANDA VIDIGAL DUARTE SOUZA, CNPMF. |
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Título: |
In vitro conservation of 'Florida Rough' lemon plants. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Ciência Rural, Santa Maria, v.52:12, e20210276, 2022. |
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ISSN: |
1678-4596 |
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DOI: |
https://doi.org/10.1590/0103-8478cr20210276 |
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Idioma: |
Inglês |
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Conteúdo: |
The establishment of minimum growth conditions is essential for in vitro germplasm conservation. Changes to the basic medium and carbon source concentrations are important factors for reducing plant growth in vitro. This study adjusted a protocol for the in vitro conservation of ?Florida Rough? lemon plants. Microcuttings (approximately 1 cm) from plants that were previously cultivated in vitro were inoculated into test tubes with 10 mL of woody plant medium (WPM) at different concentrations (1/1, 1/2 and 1/4) and supplemented with 0, 12.5, 25 and 50 g.L-1 of sucrose, solidified with 7 g.L-1 agar and adjusted to pH 5.8. The experiment was completely randomized in a 3 x 4 factorial design with 15 replications and was maintained under controlled conditions for 360 days. After this period the plant height in cm (PH), the plant dry mass in g (PDM) and the 21 numbers of green leaves (NGL), senescent leaves (NSL) and microcuttings (NM) were evaluated. The variables that best explained the observed behavior of the ?Florida Rough? lemon plants were NGL and PH, with values of 61.63 and 35.08%, respectively. The original concentration of the WPM with the addition of 25 g. L-1 of sucrose yielded the best ?Florida Rough? lemon plant growth reduction in vitro while maintaining the physiologically health of the plants. |
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Thesagro: |
Cultura In Vitro; Limão. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1149407/1/In-vitro-conservation.pdf
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Marc: |
LEADER 02052naa a2200241 a 4500
001 2149407
005 2022-12-12
008 2022 bl uuuu u00u1 u #d
022 $a1678-4596
024 7 $ahttps://doi.org/10.1590/0103-8478cr20210276$2DOI
100 1 $aCARVALHO, M. de J. da S. de
245 $aIn vitro conservation of 'Florida Rough' lemon plants.$h[electronic resource]
260 $c2022
520 $aThe establishment of minimum growth conditions is essential for in vitro germplasm conservation. Changes to the basic medium and carbon source concentrations are important factors for reducing plant growth in vitro. This study adjusted a protocol for the in vitro conservation of ?Florida Rough? lemon plants. Microcuttings (approximately 1 cm) from plants that were previously cultivated in vitro were inoculated into test tubes with 10 mL of woody plant medium (WPM) at different concentrations (1/1, 1/2 and 1/4) and supplemented with 0, 12.5, 25 and 50 g.L-1 of sucrose, solidified with 7 g.L-1 agar and adjusted to pH 5.8. The experiment was completely randomized in a 3 x 4 factorial design with 15 replications and was maintained under controlled conditions for 360 days. After this period the plant height in cm (PH), the plant dry mass in g (PDM) and the 21 numbers of green leaves (NGL), senescent leaves (NSL) and microcuttings (NM) were evaluated. The variables that best explained the observed behavior of the ?Florida Rough? lemon plants were NGL and PH, with values of 61.63 and 35.08%, respectively. The original concentration of the WPM with the addition of 25 g. L-1 of sucrose yielded the best ?Florida Rough? lemon plant growth reduction in vitro while maintaining the physiologically health of the plants.
650 $aCultura In Vitro
650 $aLimão
700 1 $aSOUZA, A. da S.
700 1 $aEMANUELA BARBOSA SANTOS
700 1 $aSOARES FILHO, W. dos S.
700 1 $aLEDO, C. A. da S.
700 1 $aCOSTA, E. M. R.
700 1 $aSOUZA, F. V. D.
773 $tCiência Rural, Santa Maria$gv.52:12, e20210276, 2022.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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| 1. |  | CARVALHO, M. de J. da S. de; SOUZA, A. da S.; EMANUELA BARBOSA SANTOS; SOARES FILHO, W. dos S.; LEDO, C. A. da S.; COSTA, E. M. R.; SOUZA, F. V. D. In vitro conservation of 'Florida Rough' lemon plants. Ciência Rural, Santa Maria, v.52:12, e20210276, 2022.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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