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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
20/10/2022 |
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Data da última atualização: |
14/11/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ALVES, M. N.; LAUDECIR L.; GIRARDI, E. A.; MIRANDA, M.; WULFF, N. A.; CARVALHO, E. V.; LOPES, SÍLVIO A.; FERRO, J. A.; OLLITRAUL, P.; PEÑA, L. |
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Afiliação: |
MÔNICA N. ALVES, FUNDO DE DEFESA DA CITRICULTURA; LAUDECIR L. RAIOL-JUNIOR, FUNDO DE DEFESA DA CITRICULTURA; EDUARDO AUGUSTO GIRARDI, CNPMF; MAÉVA MIRANDA, INSTITUT AGRO, MONTPELLIER; NELSON A. WULFF, FUNDO DE DEFESA DA CITRICULTURA; EVERTON V. CARVALHO, FUNDO DE DEFESA DA CITRICULTURA; SÍLVIO A. LOPES, FUNDO DE DEFESA DA CITRICULTURA; JESUS A. FERRO, FACULDADE DE CIÊNCIAS AGRÁRIAS E VETERINÁRIAS; PATRICK OLLITRAUL, INSTITUT, MONTPELLIER; LEANDRO PEÑA, FUNDO DE DEFESA DA CITRICULTURA. |
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Título: |
Insight into resistance to 'Candidatus Liberibacter asiaticus,' associated with Huanglongbing, in Oceanian citrus genotypes. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Frontiers in Plant Science, September 2022. |
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Idioma: |
Inglês |
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Conteúdo: |
Huanglongbing (HLB), the most destructive citrus disease, is associated with unculturable, phloem-limited Candidatus Liberibacter species, mainly Ca. L. asiaticus (Las). Las is transmitted naturally by the insect Diaphorina citri. In a previous study, we determined that the Oceanian citrus relatives Eremocitrus glauca, Microcitrus warburgiana, Microcitrus papuana, and Microcitrus australis and three hybrids among them and Citrus were full-resistant to Las. After 2 years of evaluations, leaves of those seven genotypes remained Las-free even with their susceptible rootstock being infected. However, Las was detected in their stem bark above the scion-rootstock graft union. Aiming to gain an understanding of the full-resistance phenotype, new experiments were carried out with the challenge-inoculated Oceanian citrus genotypes through which we evaluated: (1) Las acquisition by D. citri fed onto them; (2) Las infection in sweet orange plants grafted with bark or budwood from them; (3) Las infection in sweet orange plants top-grafted onto them; (4) Las infection in new shoots from rooted plants of them; and (5) Las infection in new shoots of them after drastic back-pruning. Overall, results showed that insects that fed on plants from the Oceanian citrus genotypes, their canopies, new flushes, and leaves from rooted cuttings evaluated remained quantitative real-time polymerase chain reaction (qPCR)-negative. Moreover, their budwood pieces were unable to infect sweet orange through grafting. Furthermore, sweet orange control leaves resulted infected when insects fed onto them and graft-receptor susceptible plants. Genomic and morphological analysis of the Oceanian genotypes corroborated that E. glauca and M. warburgiana are pure species while our M. australis accession is an M. australis × M. inodora hybrid and M. papuana is probably a M. papuana × M. warburgiana hybrid. E. glauca × C. sinensis hybrid was found coming from a cross between E. glauca and mandarin or tangor. Eremocitrus × Microcitrus hybrid is a complex admixture of M. australasica, M. australis, and E. glauca while the last hybrid is an M. australasica × M. australis admixture. Confirmation of consistent full resistance in these genotypes with proper validation of their genomic parentages is essential to map properly genomic regions for breeding programs aimed to generate new Citrus-like cultivars yielding immunity to HLB. MenosHuanglongbing (HLB), the most destructive citrus disease, is associated with unculturable, phloem-limited Candidatus Liberibacter species, mainly Ca. L. asiaticus (Las). Las is transmitted naturally by the insect Diaphorina citri. In a previous study, we determined that the Oceanian citrus relatives Eremocitrus glauca, Microcitrus warburgiana, Microcitrus papuana, and Microcitrus australis and three hybrids among them and Citrus were full-resistant to Las. After 2 years of evaluations, leaves of those seven genotypes remained Las-free even with their susceptible rootstock being infected. However, Las was detected in their stem bark above the scion-rootstock graft union. Aiming to gain an understanding of the full-resistance phenotype, new experiments were carried out with the challenge-inoculated Oceanian citrus genotypes through which we evaluated: (1) Las acquisition by D. citri fed onto them; (2) Las infection in sweet orange plants grafted with bark or budwood from them; (3) Las infection in sweet orange plants top-grafted onto them; (4) Las infection in new shoots from rooted plants of them; and (5) Las infection in new shoots of them after drastic back-pruning. Overall, results showed that insects that fed on plants from the Oceanian citrus genotypes, their canopies, new flushes, and leaves from rooted cuttings evaluated remained quantitative real-time polymerase chain reaction (qPCR)-negative. Moreover, their budwood pieces were unable to infect sweet orange through g... Mostrar Tudo |
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Palavras-Chave: |
HLB; Huanglongbing. |
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Thesagro: |
Doença de Planta; Fruta Cítrica. |
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Thesaurus Nal: |
Candidatus Liberibacter; Citrus; Diaphorina citri; Plant diseases and disorders. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03362naa a2200325 a 4500
001 2147499
005 2022-11-14
008 2022 bl uuuu u00u1 u #d
100 1 $aALVES, M. N.
245 $aInsight into resistance to 'Candidatus Liberibacter asiaticus,' associated with Huanglongbing, in Oceanian citrus genotypes.$h[electronic resource]
260 $c2022
520 $aHuanglongbing (HLB), the most destructive citrus disease, is associated with unculturable, phloem-limited Candidatus Liberibacter species, mainly Ca. L. asiaticus (Las). Las is transmitted naturally by the insect Diaphorina citri. In a previous study, we determined that the Oceanian citrus relatives Eremocitrus glauca, Microcitrus warburgiana, Microcitrus papuana, and Microcitrus australis and three hybrids among them and Citrus were full-resistant to Las. After 2 years of evaluations, leaves of those seven genotypes remained Las-free even with their susceptible rootstock being infected. However, Las was detected in their stem bark above the scion-rootstock graft union. Aiming to gain an understanding of the full-resistance phenotype, new experiments were carried out with the challenge-inoculated Oceanian citrus genotypes through which we evaluated: (1) Las acquisition by D. citri fed onto them; (2) Las infection in sweet orange plants grafted with bark or budwood from them; (3) Las infection in sweet orange plants top-grafted onto them; (4) Las infection in new shoots from rooted plants of them; and (5) Las infection in new shoots of them after drastic back-pruning. Overall, results showed that insects that fed on plants from the Oceanian citrus genotypes, their canopies, new flushes, and leaves from rooted cuttings evaluated remained quantitative real-time polymerase chain reaction (qPCR)-negative. Moreover, their budwood pieces were unable to infect sweet orange through grafting. Furthermore, sweet orange control leaves resulted infected when insects fed onto them and graft-receptor susceptible plants. Genomic and morphological analysis of the Oceanian genotypes corroborated that E. glauca and M. warburgiana are pure species while our M. australis accession is an M. australis × M. inodora hybrid and M. papuana is probably a M. papuana × M. warburgiana hybrid. E. glauca × C. sinensis hybrid was found coming from a cross between E. glauca and mandarin or tangor. Eremocitrus × Microcitrus hybrid is a complex admixture of M. australasica, M. australis, and E. glauca while the last hybrid is an M. australasica × M. australis admixture. Confirmation of consistent full resistance in these genotypes with proper validation of their genomic parentages is essential to map properly genomic regions for breeding programs aimed to generate new Citrus-like cultivars yielding immunity to HLB.
650 $aCandidatus Liberibacter
650 $aCitrus
650 $aDiaphorina citri
650 $aPlant diseases and disorders
650 $aDoença de Planta
650 $aFruta Cítrica
653 $aHLB
653 $aHuanglongbing
700 1 $aLAUDECIR L.
700 1 $aGIRARDI, E. A.
700 1 $aMIRANDA, M.
700 1 $aWULFF, N. A.
700 1 $aCARVALHO, E. V.
700 1 $aLOPES, SÍLVIO A.
700 1 $aFERRO, J. A.
700 1 $aOLLITRAUL, P.
700 1 $aPEÑA, L.
773 $tFrontiers in Plant Science, September 2022.
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| 2. |  | PEDROSO, M. T. M. Debatendo a expressão "agricultura familiar". In: GUEDES, I. M. R.; VIDAL, M. C. (Ed.). Curso internacional sobre produção sustentável de hortaliças: módulo 2 produção sustentável de hortaliças. Embrapa: Brasília, DF, 2012. p. 10-11.| Tipo: Capítulo em Livro Técnico-Científico |
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