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Registro Completo |
Biblioteca(s): |
Embrapa Instrumentação. |
Data corrente: |
18/03/2022 |
Data da última atualização: |
18/03/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SOARES, J. C.; SOARES, A. C.; ANGELIM, M. K. S. C.; PROENÇA-MODENA, J. L.; VIEIRA, P. M. M.; MATTOSO, L. H. C.; OLIVEIRA JR, O. N. |
Afiliação: |
LUIZ HENRIQUE CAPPARELLI MATTOSO, CNPDIA. |
Título: |
Diagnostics of SARS-CoV-2 infection using electrical impedance spectroscopy with an immunosensor to detect the spike protein. |
Ano de publicação: |
2022 |
Fonte/Imprenta: |
Talanta, v. 239, 123076, 2022. |
Páginas: |
1 - 7 |
ISSN: |
0039-9140 |
DOI: |
https://doi.org/10.1016/j.talanta.2021.123076 |
Idioma: |
Inglês |
Conteúdo: |
Mass testing for the diagnostics of COVID-19 has been hampered in many countries owing to the high cost of the methodologies to detect genetic material of SARS-CoV-2. In this paper, we report on a low-cost immunosensor capable of detecting the spike protein of SARS-CoV-2, including in samples of inactivated virus. Detection is performed with electrical impedance spectroscopy using an immunosensor that contains a monolayer film of carboxymethyl chitosan as matrix, coated with an active layer of antibodies specific to the spike protein. In addition to a low limit of detection of 0.179 fg/mL within an almost linear behavior from 10− 20 g/mL to 10− 14 g/ mL, the immunosensor was highly selective. For the samples with the spike protein could be distinguished in multidimensional projection plots from samples with other biomarkers and analytes that could be interfering species for healthy and infected patients. The excellent analytical performance of the immunosensors was validated with the distinction between control samples and those containing inactivated SARS-CoV-2 at different concentrations. The mechanism behind the immunosensor performance is the specific antibody-protein interaction, as confirmed with the changes induced in C?H stretching and protein bands in polarization-modulated infrared reflection absorption spectra (PM-IRRAS). Because impedance spectroscopy measurements can be made with low-cost portable instruments, the immunosensor proposed here can be applied in point-of-care diagnostics for mass testing even in places with limited resources. MenosMass testing for the diagnostics of COVID-19 has been hampered in many countries owing to the high cost of the methodologies to detect genetic material of SARS-CoV-2. In this paper, we report on a low-cost immunosensor capable of detecting the spike protein of SARS-CoV-2, including in samples of inactivated virus. Detection is performed with electrical impedance spectroscopy using an immunosensor that contains a monolayer film of carboxymethyl chitosan as matrix, coated with an active layer of antibodies specific to the spike protein. In addition to a low limit of detection of 0.179 fg/mL within an almost linear behavior from 10− 20 g/mL to 10− 14 g/ mL, the immunosensor was highly selective. For the samples with the spike protein could be distinguished in multidimensional projection plots from samples with other biomarkers and analytes that could be interfering species for healthy and infected patients. The excellent analytical performance of the immunosensors was validated with the distinction between control samples and those containing inactivated SARS-CoV-2 at different concentrations. The mechanism behind the immunosensor performance is the specific antibody-protein interaction, as confirmed with the changes induced in C?H stretching and protein bands in polarization-modulated infrared reflection absorption spectra (PM-IRRAS). Because impedance spectroscopy measurements can be made with low-cost portable instruments, the immunosensor proposed here can be ap... Mostrar Tudo |
Palavras-Chave: |
Impedance spectroscopy; Information visualization; SARS-CoV-2; Spike protein. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02452naa a2200277 a 4500 001 2141031 005 2022-03-18 008 2022 bl uuuu u00u1 u #d 022 $a0039-9140 024 7 $ahttps://doi.org/10.1016/j.talanta.2021.123076$2DOI 100 1 $aSOARES, J. C. 245 $aDiagnostics of SARS-CoV-2 infection using electrical impedance spectroscopy with an immunosensor to detect the spike protein.$h[electronic resource] 260 $c2022 300 $a1 - 7 520 $aMass testing for the diagnostics of COVID-19 has been hampered in many countries owing to the high cost of the methodologies to detect genetic material of SARS-CoV-2. In this paper, we report on a low-cost immunosensor capable of detecting the spike protein of SARS-CoV-2, including in samples of inactivated virus. Detection is performed with electrical impedance spectroscopy using an immunosensor that contains a monolayer film of carboxymethyl chitosan as matrix, coated with an active layer of antibodies specific to the spike protein. In addition to a low limit of detection of 0.179 fg/mL within an almost linear behavior from 10− 20 g/mL to 10− 14 g/ mL, the immunosensor was highly selective. For the samples with the spike protein could be distinguished in multidimensional projection plots from samples with other biomarkers and analytes that could be interfering species for healthy and infected patients. The excellent analytical performance of the immunosensors was validated with the distinction between control samples and those containing inactivated SARS-CoV-2 at different concentrations. The mechanism behind the immunosensor performance is the specific antibody-protein interaction, as confirmed with the changes induced in C?H stretching and protein bands in polarization-modulated infrared reflection absorption spectra (PM-IRRAS). Because impedance spectroscopy measurements can be made with low-cost portable instruments, the immunosensor proposed here can be applied in point-of-care diagnostics for mass testing even in places with limited resources. 653 $aImpedance spectroscopy 653 $aInformation visualization 653 $aSARS-CoV-2 653 $aSpike protein 700 1 $aSOARES, A. C. 700 1 $aANGELIM, M. K. S. C. 700 1 $aPROENÇA-MODENA, J. L. 700 1 $aVIEIRA, P. M. M. 700 1 $aMATTOSO, L. H. C. 700 1 $aOLIVEIRA JR, O. N. 773 $tTalanta$gv. 239, 123076, 2022.
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8. |  | ALVES, J.; PETRUCIO, M. M.; MATTEI, R. M.; MATTHIENSEN, A. Sucessão sazonal do fitoplâncton em um reservatório subtropical oligotrófico. In: CONGRESSO BRASILEIRO DE LIMNOLOGIA, 17.; CONGRESSO IBERO-AMERICANO DE LIMNOLOGIA, 2., 2019, Florianópolis. Anais... [Rio Claro, SP]: ABLmno; Concórdia: Embrapa Suínos e Aves, 2020. p. 149.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Suínos e Aves. |
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10. |  | MATTEI, R. M.; GROHSKOPF, M. A.; CORREA, J. C.; RAIZER, D.; NICOLOSO, R. da S. Cinco anos de produtividade de milho adubado com cama de aves ou fertilizantes mineral em plantio direto. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 32.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 16.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 14.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 11., 2016, Goiânia. Rumo aos novos desafios: [anais]. Viçosa, MG: Sociedade Brasileira de Ciência do Solo, 2016. Fertbio 2016.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Suínos e Aves. |
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12. |  | RAIZER, D.; HENTZ, P.; CORREA, J. C.; FONTANELI, R. S.; MATTEI, R. M. Doses de cama de aves e dejeto de suínos para sistema de integração lavoura-pecuária. In: SIMPÓSIO INTERNACIONAL SOBRE GERENCIAMENTO DE RESÍDUOS AGROPECUÁRIOS E AGROINDUSTRIAIS, 4., 2015, Rio de Janeiro, RJ. Anais... Brasília: Embrapa, 2015.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Suínos e Aves. |
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13. |  | PALHARES, J. C. P.; MULLER, S.; MATTEI, R. M.; KICH, J. D.; SIGUA, G. C. Microbiological water quality assessment of watershed associated with swine production in Santa Catarina, Brazil. In: INTERNATIONAL ANNUAL MEETINGS, 2007, New Orleans. A century of integrating crops, soils and environment: abstracts. News Orleans: ASA / CSSA / SSSA, 2007. 1 CD-ROM. Projeto n. 03.06.52.000-03.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Suínos e Aves. |
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14. |  | GROHSKOPF, M. A.; REBELLATTO, A.; CORREA, J. C.; MATTEI, R. M.; RIGO, A. Z. Fertilizantes organominerais ou minerais nas formas sólidas ou fluídas na adubação do milho. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 32.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 16.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 14.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 11., 2016, Goiânia. Rumo aos novos desafios: [anais]. Viçosa, MG: Sociedade Brasileira de Ciência do Solo, 2016. Fertbio 2016.Tipo: Resumo em Anais de Congresso |
Biblioteca(s): Embrapa Suínos e Aves. |
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15. |  | HIGARASHI, M. M.; NICOLOSO, R. da S.; OLIVEIRA, P. A. V. de; MATTEI, R. M. Greenhouse gases emissions from deep pit stored swine manure in Southern Brazil. In: SIMPÓSIO INTERNACIONAL SOBRE GERENCIAMENTO DE RESÍDUOS AGROPECUÁRIOS E AGROINDUSTRIAIS, 3., 2013, São Pedro, SP. Anais... São Pedro, SP: SBERA, 2013.Tipo: Artigo em Anais de Congresso |
Biblioteca(s): Embrapa Suínos e Aves. |
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17. |  | CORREA, J. C.; GROHSKOPF, M. A.; NICOLOSO, R. da S.; LOURENÇO, K. S.; MATTEI, R. M. Organic, organomineral, and mineral fertilizers with urease and nitrification inhibitors for wheat and corn under no-tillage. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 51, n. 8, p. 916-924, ago. 2016.Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
Biblioteca(s): Embrapa Suínos e Aves; Embrapa Unidades Centrais. |
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19. |  | MATTEI, R. M.; CESTONARO, T.; CORREA, J. C.; GROHSKOPF, M. A.; RIGO, A. Z. Resposta do feijoeiro à adubação com diferentes tipos de cama de aves. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 32.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 16.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 14.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 11., 2016, Goiânia. Rumo aos novos desafios: [anais]. Viçosa, MG: Sociedade Brasileira de Ciência do Solo, 2016. Fertbio 2016.Tipo: Resumo em Anais de Congresso |
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