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Registro Completo |
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Biblioteca(s): |
Embrapa Agroenergia; Embrapa Meio-Norte. |
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Data corrente: |
26/01/2022 |
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Data da última atualização: |
21/02/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SILVA, T. L. C. da; SILVA, V. N. B.; BRAGA, I. de O.; RODRIGUES NETO, J. C.; LEAO, A. P.; RIBEIRO, J. A. de A.; VALADARES, L. F.; ABDELNUR, P. V.; SOUSA, C. A. F. de; SOUZA JUNIOR, M. T. |
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Afiliação: |
THALLITON LUIZ CARVALHO DA SILVA, Programa de Pós-Graduação em Biotecnologia Vegetal, Universidade Federal de Lavras, MG, Brasil.; VIVIANNY NAYSE BELO SILVA, Programa de Pós-Graduação em Biotecnologia Vegetal, Universidade Federal de Lavras, MG, Brasil.; ÍTALO DE OLIVEIRA BRAGA, Programa de Pós-Graduação em Biotecnologia Vegetal, Universidade Federal de Lavras, MG, Brasil.; JORGE CANDIDO RODRIGUES NETO, Instituto de Química, Universidade Federal de Goiás, Goiânia, GO, Brasil.; ANDRE PEREIRA LEAO, CNPAE; JOSE ANTONIO DE AQUINO RIBEIRO, CNPAE; LEONARDO FONSECA VALADARES, CNPAE; PATRICIA VERARDI ABDELNUR, CNPAE; CARLOS ANTONIO FERREIRA DE SOUSA, CPAMN; MANOEL TEIXEIRA SOUZA JUNIOR, CNPAE. |
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Título: |
Integration of metabolomics and transcriptomics data to further characterize Gliricidia sepium (Jacq.) Kunth under high salinity stress. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Plant Genome, e20182, 2021. |
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Idioma: |
Inglês |
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Conteúdo: |
Soil salinity is one abiotic stress that threatens agriculture in more than 100 countries. Gliricidia [Gliricidia sepium (Jacq.) Kunth] is a multipurpose tree known for its ability to adapt to a wide range of soils; however, its tolerance limits and responses to salt stress are not yet well understood. In this study, after characterizing the morphophysiological responses of young gliricidia plants to salinity stress, leaf metabolic and transcription profiles were generated and submitted to single and integrated analyses. RNA from leaf samples were subjected to RNA sequencing using an Illumina HiSeq platform and the paired-end strategy. Polar and lipidic fractions from leaf samples were extracted and analyzed on an ultra-high-performance liquid chromatography (UHPLC) coupled with electrospray ionization quadrupole time-offlight high-resolution mass spectrometry (MS) system. Acquired data were analyzed using the OmicsBox, XCMS Online, MetaboAnalyst, and Omics Fusion platforms. The substrate salinization protocol used allowed the identification of two distinct responses to salt stress: tolerance and adaptation. Single analysis on transcriptome and metabolome data sets led to a group of 5,672 transcripts and 107 metabolites differentially expressed in gliricidia leaves under salt stress. The phenylpropanoid biosynthesis was the most affected pathway, with 15 metabolites and three genes differentially expressed. Results showed that the differentially expressed metabolites and genes from this pathway affect mainly short-term salt stress (STS). The single analysis of the transcriptome identified 12 genes coding for proteins that might play a role in gliricidia response at both STS and long-termsalt stress (LTS). Further studies are needed to reveal the mechanisms behind the adaptation response. MenosSoil salinity is one abiotic stress that threatens agriculture in more than 100 countries. Gliricidia [Gliricidia sepium (Jacq.) Kunth] is a multipurpose tree known for its ability to adapt to a wide range of soils; however, its tolerance limits and responses to salt stress are not yet well understood. In this study, after characterizing the morphophysiological responses of young gliricidia plants to salinity stress, leaf metabolic and transcription profiles were generated and submitted to single and integrated analyses. RNA from leaf samples were subjected to RNA sequencing using an Illumina HiSeq platform and the paired-end strategy. Polar and lipidic fractions from leaf samples were extracted and analyzed on an ultra-high-performance liquid chromatography (UHPLC) coupled with electrospray ionization quadrupole time-offlight high-resolution mass spectrometry (MS) system. Acquired data were analyzed using the OmicsBox, XCMS Online, MetaboAnalyst, and Omics Fusion platforms. The substrate salinization protocol used allowed the identification of two distinct responses to salt stress: tolerance and adaptation. Single analysis on transcriptome and metabolome data sets led to a group of 5,672 transcripts and 107 metabolites differentially expressed in gliricidia leaves under salt stress. The phenylpropanoid biosynthesis was the most affected pathway, with 15 metabolites and three genes differentially expressed. Results showed that the differentially expressed metabolites and gen... Mostrar Tudo |
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Palavras-Chave: |
Adaptation; Salinization protocol. |
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Categoria do assunto: |
-- |
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URL: |
https://www.alice.cnptia.embrapa.br/alice/bitstream/doc/1139329/1/The-Plant-Genome-2022-Integration.pdf
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Marc: |
LEADER 02601naa a2200253 a 4500
001 2139329
005 2022-02-21
008 2021 bl uuuu u00u1 u #d
100 1 $aSILVA, T. L. C. da
245 $aIntegration of metabolomics and transcriptomics data to further characterize Gliricidia sepium (Jacq.) Kunth under high salinity stress.$h[electronic resource]
260 $c2021
520 $aSoil salinity is one abiotic stress that threatens agriculture in more than 100 countries. Gliricidia [Gliricidia sepium (Jacq.) Kunth] is a multipurpose tree known for its ability to adapt to a wide range of soils; however, its tolerance limits and responses to salt stress are not yet well understood. In this study, after characterizing the morphophysiological responses of young gliricidia plants to salinity stress, leaf metabolic and transcription profiles were generated and submitted to single and integrated analyses. RNA from leaf samples were subjected to RNA sequencing using an Illumina HiSeq platform and the paired-end strategy. Polar and lipidic fractions from leaf samples were extracted and analyzed on an ultra-high-performance liquid chromatography (UHPLC) coupled with electrospray ionization quadrupole time-offlight high-resolution mass spectrometry (MS) system. Acquired data were analyzed using the OmicsBox, XCMS Online, MetaboAnalyst, and Omics Fusion platforms. The substrate salinization protocol used allowed the identification of two distinct responses to salt stress: tolerance and adaptation. Single analysis on transcriptome and metabolome data sets led to a group of 5,672 transcripts and 107 metabolites differentially expressed in gliricidia leaves under salt stress. The phenylpropanoid biosynthesis was the most affected pathway, with 15 metabolites and three genes differentially expressed. Results showed that the differentially expressed metabolites and genes from this pathway affect mainly short-term salt stress (STS). The single analysis of the transcriptome identified 12 genes coding for proteins that might play a role in gliricidia response at both STS and long-termsalt stress (LTS). Further studies are needed to reveal the mechanisms behind the adaptation response.
653 $aAdaptation
653 $aSalinization protocol
700 1 $aSILVA, V. N. B.
700 1 $aBRAGA, I. de O.
700 1 $aRODRIGUES NETO, J. C.
700 1 $aLEAO, A. P.
700 1 $aRIBEIRO, J. A. de A.
700 1 $aVALADARES, L. F.
700 1 $aABDELNUR, P. V.
700 1 $aSOUSA, C. A. F. de
700 1 $aSOUZA JUNIOR, M. T.
773 $tPlant Genome, e20182, 2021.
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Registro original: |
Embrapa Agroenergia (CNPAE) |
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| 5. |  | BACCHIN, A. L. G.; CUNHA, F. M. da; ZANGIROLAMI, T. C.; FARINAS, C. S. Quantificação indireta do crescimento celular de aspergillus niger em meio contendo bagaço de cana-de-açúcar visando fermentação em biorreator do tipo airlift. In: JORNADA CIENTÍFICA - EMBRAPA SÃO CARLOS, 2., 2010, São Carlos, SP. Anais... São Carlos: Embrapa Instrumentação Agropecuária: Embrapa Pecuária Sudeste, 2010. p. 109. (Embrapa Instrumentação Agropecuária. Documentos, 50).| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação. |
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| 6. | | CUNHA, F. M.; BACCHIN, A. L. G.; HORTA, A. C. L.; ZANGIROLAMI, T. C.; BADINO, A. C.; FARINAS, C. S. Indirect method for quantification of cellular biomass in a solids-containing medium used as pre-culture for cellulase production. Biotechnology and Bioprocess Engineering, [S. l.], v. 17, p. 100-108, 2012.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
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| Registros recuperados : 6 | |
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