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Biblioteca(s): |
Embrapa Clima Temperado. |
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Data corrente: |
29/11/2021 |
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Data da última atualização: |
29/11/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MAIA, D. S. V.; HAUBERT, L.; WÜRFEL, S. de F. R.; KRONING, I. S.; CARDOSO, M. R. de I.; LOPES, G. V.; FIORENTINI, A. M.; SILVA, W. P. |
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Afiliação: |
DARLA SILVEIRA VOLCAN MAIA; LOUISE HAUBERT; SIMONE DE FATIMA RAUBER WÜRFEL; ISABELA SCHNEID KRONING; MARISA RIBEIRO DE ITAPEMA CARDOSO; GRACIELA VÖLZ LOPES; Â NGELA MARIA FIORENTINI; WLADIMIR PADILHA DA SILVA. |
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Título: |
Listeria monocytogenes in sliced cheese and ham from retail markets in southern Brazil. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
FEMS Microbiology Letters, v. 366, n. 22,p. 1-6, 2019. |
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Idioma: |
Inglês |
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Conteúdo: |
ABSTRACT The aims of this study were to evaluate the occurrence of Listeria monocytogenes and Salmonella spp. in sliced cheese and ham from retail markets in southern Brazil, as well as to perform molecular characterization and to assess the antimicrobial resistance profile of the isolates. Samples (n = 160) of sliced cheese and ham were collected at retail level from the city of Pelotas, Brazil. The isolation of L. monocytogenes and Salmonella spp. was performed and the isolates were confirmed by PCR, submitted to antimicrobial susceptibility testing and pulsed-field gel electrophoresis (PFGE). Listeria monocytogenes was found in 9.4% (15/160) of the samples. All L. monocytogenes isolates were positive for the prs, inlA, inlC and inlJ genes. Salmonella spp. was not isolated. Regarding the antimicrobial susceptibility, one (6.6%) L. monocytogenes isolate was resistant to streptomycin and four (26.6%) to clindamycin. Macrorestriction analysis with ApaI and AscI enzymes yielded two major PFGE groups I and II. All L. monocytogenes isolates showed virulence genes, and some of them were resistant to clinically used antimicrobials, representing a risk to public health. Moreover, PFGE patterns with high similarity were visualized in L. monocytogenes isolates at different times, demonstrating adaptability of the pathogen at retail level in the region. |
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Palavras-Chave: |
Antimicrobial resistance; Foodborne pathogen; PFGE RTE foods; Serotyping; Virulence genes. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02145naa a2200265 a 4500
001 2136775
005 2021-11-29
008 2019 bl uuuu u00u1 u #d
100 1 $aMAIA, D. S. V.
245 $aListeria monocytogenes in sliced cheese and ham from retail markets in southern Brazil.$h[electronic resource]
260 $c2019
520 $aABSTRACT The aims of this study were to evaluate the occurrence of Listeria monocytogenes and Salmonella spp. in sliced cheese and ham from retail markets in southern Brazil, as well as to perform molecular characterization and to assess the antimicrobial resistance profile of the isolates. Samples (n = 160) of sliced cheese and ham were collected at retail level from the city of Pelotas, Brazil. The isolation of L. monocytogenes and Salmonella spp. was performed and the isolates were confirmed by PCR, submitted to antimicrobial susceptibility testing and pulsed-field gel electrophoresis (PFGE). Listeria monocytogenes was found in 9.4% (15/160) of the samples. All L. monocytogenes isolates were positive for the prs, inlA, inlC and inlJ genes. Salmonella spp. was not isolated. Regarding the antimicrobial susceptibility, one (6.6%) L. monocytogenes isolate was resistant to streptomycin and four (26.6%) to clindamycin. Macrorestriction analysis with ApaI and AscI enzymes yielded two major PFGE groups I and II. All L. monocytogenes isolates showed virulence genes, and some of them were resistant to clinically used antimicrobials, representing a risk to public health. Moreover, PFGE patterns with high similarity were visualized in L. monocytogenes isolates at different times, demonstrating adaptability of the pathogen at retail level in the region.
653 $aAntimicrobial resistance
653 $aFoodborne pathogen
653 $aPFGE RTE foods
653 $aSerotyping
653 $aVirulence genes
700 1 $aHAUBERT, L.
700 1 $aWÜRFEL, S. de F. R.
700 1 $aKRONING, I. S.
700 1 $aCARDOSO, M. R. de I.
700 1 $aLOPES, G. V.
700 1 $aFIORENTINI, A. M.
700 1 $aSILVA, W. P.
773 $tFEMS Microbiology Letters$gv. 366, n. 22,p. 1-6, 2019.
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Embrapa Clima Temperado (CPACT) |
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| 1. |  | PIMENTA-MARTINS, M. G. R.; FURTADO, R. F.; DUTRA, R. F.; HENEINE, L. G. D.; DIAS. R. S.; BORGES, M. de F.; ALVES, C. R. Immunosensor based on ink printed electrode for staphylococcal enterotoxin detection. Revista da Universidade Vale do Rio Verde, Três Corações, v. 12, n. 1, p. 895-904, 2014.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 4 |
| Biblioteca(s): Embrapa Agroindústria Tropical. |
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| 4. | | PIMENTA-MARTINS, M. G. R.; FURTADO, R. F.; BORGES, M. de F.; HENEINE, L. G. D.; MATTOSO, L. H. C.; HERRMANN, P. S. de P.; OLIVEIRA, J. E. de; ALVES, C. R. Imunossensor amperométrico para detecção de enterotoxina estafilocócica em queijo. In: WORKSHOP DA REDE DE NANOTECNOLOGIA APLICADA AO AGRONEGÓCIO, 6., 2012, Fortaleza. Anais... São Carlos: Embrapa Instrumentação; Fortaleza: Embrapa Agroindústria Tropical, 2012. p. 76-78. Editores: Maria Alice Martins, MOrsyleide de Freitas Rosa, Men de Sá Moreira de Souza Filho, Nicodemos Moreira dos Santos Junior, Odílio Benedito Garrido de Assis, Caue Ribeiro, Luiz Henrique Capparelli Mattoso.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Instrumentação. |
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| Registros recuperados : 4 | |
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