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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
25/11/2021 |
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Data da última atualização: |
25/11/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OLIVEIRA, C. S.; MARQUES, S. C. S.; GUEDES, P. H. E.; FEUCHARD, V. L.; CAMARGO, A. J. R.; FREITAS, C. de; CAMARGO, L. S. de A. |
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Afiliação: |
CLARA SLADE OLIVEIRA, CNPGL; SHEILA C. S. MARQUES; PEDRO H. E. GUEDES; VIVIANE L. FEUCHARD, Pesagro; AGOSTINHO J. R. CAMARGO, Pesagro; CELIO DE FREITAS, CNPGL; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL. |
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Título: |
Thermal-treatment protocol to induce thermotolerance in bovine embryos. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Reproduction, Fertility and Development, v. 33, n. 7, p. 497-501, 2021. |
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DOI: |
https://doi.org/10.1071/RD20309 |
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Idioma: |
Inglês |
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Conteúdo: |
Artificial reproduction in dairy cattle is challenged by summer temperatures in tropical environments. We describe a treatment based on mild temperature increasesto induce thermotolerance and improve the embryo?s performance under heat stress conditions. A protocol was established to induce upregulation of heat shock protein A (HSPA, formerly known as HSP70) but not impair embryonic development. Thermal treatment(TT) had no effect onmorula/blastocyst rate or blastocyst quality (cell number and apoptosis). Heatshock given one day after TTrevealed higher(P ¼ 0.00)survivalratesin TT blastocysts compared with Control. Treated embryos were transferred to recipients and no detrimental effects were observed regarding pregnancy rates, length, fetal growth or calf weight. Our results demonstrated that the established TT protocol could induce a thermal response by the embryo and is safe for further development. |
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Palavras-Chave: |
Choque térmico; Embryo survival; Estresse térmico; HSP; IVF; IVP; Sobrevivência embrionária. |
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Thesagro: |
Bovino. |
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Thesaurus Nal: |
Heat shock response; Thermal stress. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
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001 2136546
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008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1071/RD20309$2DOI
100 1 $aOLIVEIRA, C. S.
245 $aThermal-treatment protocol to induce thermotolerance in bovine embryos.$h[electronic resource]
260 $c2021
520 $aArtificial reproduction in dairy cattle is challenged by summer temperatures in tropical environments. We describe a treatment based on mild temperature increasesto induce thermotolerance and improve the embryo?s performance under heat stress conditions. A protocol was established to induce upregulation of heat shock protein A (HSPA, formerly known as HSP70) but not impair embryonic development. Thermal treatment(TT) had no effect onmorula/blastocyst rate or blastocyst quality (cell number and apoptosis). Heatshock given one day after TTrevealed higher(P ¼ 0.00)survivalratesin TT blastocysts compared with Control. Treated embryos were transferred to recipients and no detrimental effects were observed regarding pregnancy rates, length, fetal growth or calf weight. Our results demonstrated that the established TT protocol could induce a thermal response by the embryo and is safe for further development.
650 $aHeat shock response
650 $aThermal stress
650 $aBovino
653 $aChoque térmico
653 $aEmbryo survival
653 $aEstresse térmico
653 $aHSP
653 $aIVF
653 $aIVP
653 $aSobrevivência embrionária
700 1 $aMARQUES, S. C. S.
700 1 $aGUEDES, P. H. E.
700 1 $aFEUCHARD, V. L.
700 1 $aCAMARGO, A. J. R.
700 1 $aFREITAS, C. de
700 1 $aCAMARGO, L. S. de A.
773 $tReproduction, Fertility and Development$gv. 33, n. 7, p. 497-501, 2021.
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Embrapa Gado de Leite (CNPGL) |
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| 1. |  | MUNIZ, E. C.; PICKLER, L.; LOURENÇO, M. C.; KRAIESKI, A. L.; MESA, D.; WESTPHAL, P.; SANTINI, E. Avaliação da resposta imunológica da mucosa intestinal de frangos de corte desafiados com diferentes sorovares de Salmonella. Pesquisa Veterinária Brasileira, Brasília, DF, v. 35, n. 3, p. 241-248, mar. 2015.| Biblioteca(s): Embrapa Unidades Centrais. |
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