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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
05/11/2021 |
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Data da última atualização: |
05/11/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OKINO, C. H.; CAMINOTTO, L. C. F.; KAPRITCHKOFF, R. T. I.; NICIURA, S. C. M.; ESTEVES, S. N.; SANTOS, I. B. DOS; TOSCANO, J. H. B.; OLIVEIRA, M. C. de S.; CHAGAS, A. C. de S. |
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Afiliação: |
CINTIA HIROMI OKINO, CPPSE; LARA CRISTINA FRIZONI CAMINOTTO, UNICEP; RAFAELA TAMI IKEDA KAPRITCHKOFF, UNESP; SIMONE CRISTINA MEO NICIURA, CPPSE; SERGIO NOVITA ESTEVES, CPPSE; ISABELLA BARBOSA DOS SANTOS, UNESP; JOAO HENRIQUE BARBOSA TOSCANO, UNESP; MARCIA CRISTINA DE SENA OLIVEIRA, CPPSE; ANA CAROLINA DE SOUZA CHAGAS, CPPSE. |
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Título: |
Comparison of ovine B-globin haplotype sequences and a new multiplex PCR for identification. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Veterinary Parasitology, v.300, 109592, dec. 2021. |
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Páginas: |
5 p. |
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DOI: |
https://doi.org/10.1016/j.vetpar.2021.109592 |
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Idioma: |
Inglês |
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Conteúdo: |
Deletion of pre-adult BC-globin in sheep harboring BB haplotype of B-globin was associated to decreased tolerance to anemia and hypoxia, and consequently, reduced resistance to Haemonchus contortus infection, which is closely related to severe anemia. Recently, a qPCR using hydrolysis probe was successfully developed for B-globin haplotype identification, and association between resistance against H. contortus and presence of ?A allele was observed in Morada Nova sheep. Thus, this study aimed to better investigate the differences between B-globin haplotypes and to develop a conventional multiplex PCR, as an alternative to qPCR assay for B-globin haplotype identification. A total of 333 Morada Nova lambs had their blood collected and tested by both qPCR and new multiplex PCR, and 100 % of agreement was observed between the results. Since different primers were designed for such assay development, including different target genes, high specificity of both methods may be also highlighted. Three A haplotype samples were submitted to DNA Sanger sequencing of B-globin gene and compared to sequences previously deposited in Genbank. One nucleotide deletion in intronic region was observed only in AA haplotype of Morada Nova animals, while in BB animals the nucleotide remained present. To the best of our knowledge, this is the first report of multiplex conventional PCR for ovine B-globin haplotype identification. The advantages of the developed conventional PCR are reduced reagents costs (less than a half price) and wider reachability, since even labs without real time PCR thermocyclers are able to offer this assay. Therefore, it may become an important tool for sheep producers to improve genetic selection of parasite resistant animals. MenosDeletion of pre-adult BC-globin in sheep harboring BB haplotype of B-globin was associated to decreased tolerance to anemia and hypoxia, and consequently, reduced resistance to Haemonchus contortus infection, which is closely related to severe anemia. Recently, a qPCR using hydrolysis probe was successfully developed for B-globin haplotype identification, and association between resistance against H. contortus and presence of ?A allele was observed in Morada Nova sheep. Thus, this study aimed to better investigate the differences between B-globin haplotypes and to develop a conventional multiplex PCR, as an alternative to qPCR assay for B-globin haplotype identification. A total of 333 Morada Nova lambs had their blood collected and tested by both qPCR and new multiplex PCR, and 100 % of agreement was observed between the results. Since different primers were designed for such assay development, including different target genes, high specificity of both methods may be also highlighted. Three A haplotype samples were submitted to DNA Sanger sequencing of B-globin gene and compared to sequences previously deposited in Genbank. One nucleotide deletion in intronic region was observed only in AA haplotype of Morada Nova animals, while in BB animals the nucleotide remained present. To the best of our knowledge, this is the first report of multiplex conventional PCR for ovine B-globin haplotype identification. The advantages of the developed conventional PCR are reduced reagents co... Mostrar Tudo |
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Palavras-Chave: |
Allele; B haplotype; Beta globin C; Morada Nova; Resistance. |
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Thesaurus Nal: |
Sheep. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02671naa a2200313 a 4500
001 2135834
005 2021-11-05
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.vetpar.2021.109592$2DOI
100 1 $aOKINO, C. H.
245 $aComparison of ovine B-globin haplotype sequences and a new multiplex PCR for identification.$h[electronic resource]
260 $c2021
300 $a5 p.
520 $aDeletion of pre-adult BC-globin in sheep harboring BB haplotype of B-globin was associated to decreased tolerance to anemia and hypoxia, and consequently, reduced resistance to Haemonchus contortus infection, which is closely related to severe anemia. Recently, a qPCR using hydrolysis probe was successfully developed for B-globin haplotype identification, and association between resistance against H. contortus and presence of ?A allele was observed in Morada Nova sheep. Thus, this study aimed to better investigate the differences between B-globin haplotypes and to develop a conventional multiplex PCR, as an alternative to qPCR assay for B-globin haplotype identification. A total of 333 Morada Nova lambs had their blood collected and tested by both qPCR and new multiplex PCR, and 100 % of agreement was observed between the results. Since different primers were designed for such assay development, including different target genes, high specificity of both methods may be also highlighted. Three A haplotype samples were submitted to DNA Sanger sequencing of B-globin gene and compared to sequences previously deposited in Genbank. One nucleotide deletion in intronic region was observed only in AA haplotype of Morada Nova animals, while in BB animals the nucleotide remained present. To the best of our knowledge, this is the first report of multiplex conventional PCR for ovine B-globin haplotype identification. The advantages of the developed conventional PCR are reduced reagents costs (less than a half price) and wider reachability, since even labs without real time PCR thermocyclers are able to offer this assay. Therefore, it may become an important tool for sheep producers to improve genetic selection of parasite resistant animals.
650 $aSheep
653 $aAllele
653 $aB haplotype
653 $aBeta globin C
653 $aMorada Nova
653 $aResistance
700 1 $aCAMINOTTO, L. C. F.
700 1 $aKAPRITCHKOFF, R. T. I.
700 1 $aNICIURA, S. C. M.
700 1 $aESTEVES, S. N.
700 1 $aSANTOS, I. B. DOS
700 1 $aTOSCANO, J. H. B.
700 1 $aOLIVEIRA, M. C. de S.
700 1 $aCHAGAS, A. C. de S.
773 $tVeterinary Parasitology$gv.300, 109592, dec. 2021.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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| Registros recuperados : 21 | |
| 2. |  | PIZOLOTTO, C. A.; MACIEL, J. L. N.; FERNANDES, J. M. C. Sobrevivência saprofítica de Magnaporthe oryzae em restos culturais de trigo. In: MOSTRA DE INICIAÇÃO CIENTÍFICA, 11., MOSTRA DE PÓS-GRADUAÇÃO DA EMBRAPA TRIGO, 8., 2016, Passo Fundo. Resumos... Brasília, DF: Embrapa, 2016. p. 53.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Trigo. |
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| 3. | | PIZOLOTTO, C. A.; MACIEL, J. L. N.; FERNANDES, J. M. C.; BOLLER, W. Saprotrophic survival of Magnaporthe oryzae in infested wheat residues. In: INTERNATIONAL SYMPOSIUM ON FUSARIUM HEAD BLIGHT, 5.; INTERNATIONAL WORKSHOP ON WHEAT BLAST, 2., 2016, Florianópolis. Book of abstracts... Passo Fundo: Ed. Universidade de Passo Fundo: Embrapa Trigo; Viçosa, MG: Universidade Federal de Viçosa, 2016. p. 150, abstr. P86. 2nd International Workshop on Wheat Blast.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Trigo. |
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| 8. | | GARCEZ, J. G.; DE MORI, C.; TOMM, G. O.; PIZOLOTTO, C. A.; BOLLER, W.; SCARANTTI, V. Custo de produção de canola: estudo de caso de propriedade em Bossoroca, RS, safra 2013. In: SIMPÓSIO LATINO AMERICANO DE CANOLA, 1., 2014, Passo Fundo. Anais... Brasília, DF: Embrapa, 2014. 5 p. Poster 4.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Trigo. |
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| 10. | | PIZOLOTTO, C. A.; MACIEL, J. L. N.; FERNANDES, J. M. C.; BOLLER, W. In vivo sensitivity of wheat Magnaporthe oryzae to fungicides. In: INTERNATIONAL SYMPOSIUM ON FUSARIUM HEAD BLIGHT, 5.; INTERNATIONAL WORKSHOP ON WHEAT BLAST, 2., 2016, Florianópolis. Book of abstracts... Passo Fundo: Ed. Universidade de Passo Fundo: Embrapa Trigo; Viçosa, MG: Universidade Federal de Viçosa, 2016. p. 151, abstr. P87. 2nd International Workshop on Wheat Blast.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Trigo. |
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| 12. | | FERREIRA, A.; SBALCHEIRO, C. C.; VENÂNCIO, W. S.; PIZOLOTTO, C.; DALLAGNOL, L. J.; CASAROTTO, G.; ZANATTA, M.; CÓL, M. C. de; GUTERRES, C. W.; DEL PONTE, E. M. Eficiência de fungicidas para controle de oídio do trigo na Rede de Ensaios Cooperativos, safra 2024. Passo Fundo: Embrapa Trigo, 2024. (Embrapa Trigo. Boletim de pesquisa e desenvolvimento, 126).| Tipo: Boletim de Pesquisa e Desenvolvimento |
| Biblioteca(s): Embrapa Trigo. |
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| 13. | | SANTANA, F. M.; SBALCHEIRO, C. C.; VENANCIO, W. S.; UTIAMADA, C. M.; SENGER, M.; CHAGAS, D. F.; PIZOLOTTO, C.; CASAROTTO, G.; COSTA, A. A. Eficiência de fungicidas para controle de oídio do trigo: resultados dos ensaios cooperativos, safra 2021. Passo Fundo: Embrapa Trigo, 2023. 20 p. (Embrapa Trigo. Circular técnica, 83).| Biblioteca(s): Embrapa Trigo. |
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| 14. | | FERREIRA, A.; SBALCHEIRO, C. C.; DEL PONTE, E. M.; CÓL, M. C. de; SUSSEL, A. A. B.; UTIAMADA, C. M.; PIZOLOTTO, C.; DALLAGNOL, L. J.; VENANCIO, W. S. Eficiência de fungicidas para manchas foliares do trigo: Rede de Ensaios Cooperativos, safra 2023. Passo Fundo: Embrapa Trigo, 2024. 9 p. (Embrapa Trigo. Boletim de pesquisa e desenvolvimento, 115).| Tipo: Boletim de Pesquisa e Desenvolvimento |
| Biblioteca(s): Embrapa Trigo. |
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| 15. | | PIZOLOTTO, C. A.; MACIEL, J. L. N.; FERNANDES, J. M. C.; FERREIRA, J. R.; TORRES, G. A. M.; CONSOLI, L.; BOLLER, W. Reação à brusone de genótipos de trigo portadores da translocação 2NS/AS de Aegilops ventricosa. In: REUNIÃO DA COMISSÃO BRASILEIRA DE PESQUISA DE TRIGO E TRITICALE, 11.; FÓRUM NACIONAL DE TRIGO, 2017, Cascavel. Resumos expandidos... Cascavel: Coodetec, 2017. p. 151-155.| Tipo: Artigo em Anais de Congresso |
| Biblioteca(s): Embrapa Trigo. |
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| 16. | | SANTANA, F. M.; THEISEN, G.; SBALCHEIRO, C. C.; SENGER, M.; CHAGAS, D. F.; SCHIPANSKI, C. A.; CASAROTTO, G.; VENANCIO, W. S.; PIZOLOTTO, C.; UTIAMADA, C. M.; COSTA, A. A.; PADUA, J. M. V.; GUTERRES, C. W. Eficiência de fungicidas para controle de manchas foliares do trigo: resultados da Rede de Ensaios Cooperativos do Trigo, safras 2020 e 2021. Pelotas: Embrapa Clima Temperado, 2024. 17 p. (Embrapa Clima Temperado. Boletim de Pesquisa e Desenvolvimento, 372).| Tipo: Boletim de Pesquisa e Desenvolvimento |
| Biblioteca(s): Embrapa Clima Temperado. |
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| 17. | | FERREIRA, A.; SBALCHEIRO, C. C.; SANTANA, F. M.; DEL PONTE, E. M.; SENGER, M.; GUTERRES, C. W.; PIZOLOTTO, C.; VENANCIO, W. S.; MARTINS, F. C.; KUHNEM, P. R.; CHAGAS, D. F.; SCHIPANSKI, C. A.; ZANATTA, M.; CASAROTTO, G.; MACHADO, F. J.; FORCELINI, C. A.; ROEHRIG, R.; CÓL, M. C. de. Eficiência de fungicidas para controle de giberela do trigo: resultados da Rede de Ensaios Cooperativos do Trigo - safra 2022. Passo Fundo: Embrapa Trigo, 2023. 18 p. (Embrapa Trigo. Circular técnica, 80). ODS 2. ODS 12.| Biblioteca(s): Embrapa Clima Temperado; Embrapa Trigo. |
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| 18. | | FERREIRA, A.; SBALCHEIRO, C. C.; SANTANA, F. M.; DEL PONTE, E. M.; CASAROTTO, G.; CHAGAS, D. F.; SCHIPANSKI, C. A.; PIZOLOTTO, C.; VENANCIO, W. S.; GUTERRES, C. W.; SENGER, M.; FORCELINI, C. A.; ROEHRIG, R.; ZANATTA, M.; PÁDUA, J. M. V.; UTIAMADA, C. M.; SUSSEL, A. A. B.; CÓL, M. C. de. Eficiência de fungicidas para controle de manchas foliares do trigo: resultados da Rede de Ensaios Cooperativos do Trigo - safra 2022. Passo Fundo: Embrapa Trigo, 2023. 16 p. (Embrapa Trigo. Circular técnica, 82). ODS 2. ODS 12.| Biblioteca(s): Embrapa Trigo. |
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| 19. | | MEYER, M. C.; CAMPOS, H. D.; GODOY, C. V.; UTIAMADA, C. M.; SATO, L. N.; CHAGAS, D. F.; SCHIPANSKI, C. A.; GALDINO, J. V.; SENGER, M.; BRUSTOLIN, R.; PIZOLOTTO, C. A.; DIAS, A. R.; NUNES JUNIOR, J.; LOBO JUNIOR, M.; TORMEN, N. R.; JULIATTI, F. C.; MARTINS, M. C.; SOUZA, T. P. de; OLIVEIRA, M. C. N. de. Eficiência de fungicidas para controle de mofo-branco (Sclerotinia sclerotiorum) em soja, na safra 2021/2022: resultados sumarizados dos experimentos cooperativos. Londrina: Embrapa Soja, 2022. 14 p. (Embrapa Soja. Circular técnica, 189).| Biblioteca(s): Embrapa Arroz e Feijão; Embrapa Soja. |
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| 20. | | FERREIRA, A.; SBALCHEIRO, C. C.; SANTANA, F. M.; DEL PONTE, E. M.; CHAGAS, D. F.; SCHIPANSKI, C. A.; PIZOLOTTO, C.; VENANCIO, W. S.; CASAROTTO, G.; SENGER, M.; GUTERRES, C. W.; MUHL, A.; ZANATTA, M.; UTIAMADA, C. M.; FORCELINI, C. A.; ROEHRIG, R.; CÓL, M. C. de. Eficiência de fungicidas para controle de oídio do trigo: resultados da Rede de Ensaios Cooperativos do Trigo - safra 2022. Passo Fundo: Embrapa Trigo, 2023. 16 p. (Embrapa Trigo. Circular técnica, 81). ODS 2. ODS 12.| Biblioteca(s): Embrapa Trigo. |
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| Registros recuperados : 21 | |
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