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Registro Completo |
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Biblioteca(s): |
Embrapa Instrumentação. |
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Data corrente: |
19/10/2021 |
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Data da última atualização: |
09/06/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SOARES, J. C.; SOARES, A. C.; RODRIGUES, V. C.; OITICICA, P. R. A.; RAYMUNDO-PEREIRA, P. A.; BOTT-NETO, J. L.; BUSCAGLIA, L. A.; CASTRO, L. D. C. de; RIBAS, L. C.; SCABINI, L.; BRAZACA, L. C.; CORREA, D. S.; MATTOSO, L. H. C.; OLIVEIRA, M. C. F. de; CARVALHO, A. C. P. L. de; CARRILHO, E.; BRUNO, O. M.; MELENDEZ, M. E.; OLIVEIRA JR, O. N. |
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Afiliação: |
DANIEL SOUZA CORREA, CNPDIA; LUIZ HENRIQUE CAPPARELLI MATTOSO, CNPDIA. |
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Título: |
Detection of a SARS-CoV-2 sequence with genosensors using data analysis based on information visualization and machine learning techniques. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Materials Chemistry Frontiers, v. 5, 2021. |
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Páginas: |
5658-5670 |
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DOI: |
10.1039/d1qm00665g |
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Idioma: |
Português |
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Conteúdo: |
We report on genosensors to detect an ssDNA sequence from the SARS-CoV-2 genome, which mimics the GU280 gp10 gene (coding the viral nucleocapsid phosphoprotein), using four distinct principles of detection and treating the data with information visualization and machine learning techniques. Genosensors were fabricated on either gold (Au) interdigitated electrodes for electrical and electrochemical measurements or on Au nanoparticles on a glass slide for optical measurements. They contained a matrix of 11-mercaptoundecanoic acid (11-MUA) self-assembled monolayer (SAM) onto which a layer of capture probe (cpDNA) sequence was immobilized. Detection was performed using electrical and electrochemical impedance spectroscopies and localized surface plasmon resonance (LSPR). The highest sensitivity was reached with impedance spectroscopy, including using a low-cost (US$ 100) homemade impedance analyzer. Complementary ssDNA sequences were detected with a detection limit of 0.5 aM (0.3 copy per mL). This performance may be attributed to the high sensitivity of the electrical impedance technique combined with an appropriate arrangement of the sequences on the electrodes and hybridization between the complementary sequences, as inferred from polarizationmodulated infrared reflection absorption spectroscopy (PM-IRRAS). The selectivity of the genosensor was confirmed by plotting the impedance spectroscopy data with a multidimensional projection technique (interactive document mapping, IDMAP), where a clear separation was observed among the samples of the complementary DNA sequence at various concentrations and from buffer samples containing a non-complementary sequence and other DNA biomarkers. The diagnosis of SARS-CoV-2 mimicking sequences was also achieved with machine learning techniques applied to scanning electron microscope images taken from genosensors exposed to distinct concentrations of the complementary ssDNA sequences. In summary, the genosensors proposed here are promising for detecting SARS-CoV2 genetic material (RNA) in biological fluids in point-of-care settings MenosWe report on genosensors to detect an ssDNA sequence from the SARS-CoV-2 genome, which mimics the GU280 gp10 gene (coding the viral nucleocapsid phosphoprotein), using four distinct principles of detection and treating the data with information visualization and machine learning techniques. Genosensors were fabricated on either gold (Au) interdigitated electrodes for electrical and electrochemical measurements or on Au nanoparticles on a glass slide for optical measurements. They contained a matrix of 11-mercaptoundecanoic acid (11-MUA) self-assembled monolayer (SAM) onto which a layer of capture probe (cpDNA) sequence was immobilized. Detection was performed using electrical and electrochemical impedance spectroscopies and localized surface plasmon resonance (LSPR). The highest sensitivity was reached with impedance spectroscopy, including using a low-cost (US$ 100) homemade impedance analyzer. Complementary ssDNA sequences were detected with a detection limit of 0.5 aM (0.3 copy per mL). This performance may be attributed to the high sensitivity of the electrical impedance technique combined with an appropriate arrangement of the sequences on the electrodes and hybridization between the complementary sequences, as inferred from polarizationmodulated infrared reflection absorption spectroscopy (PM-IRRAS). The selectivity of the genosensor was confirmed by plotting the impedance spectroscopy data with a multidimensional projection technique (interactive document mapping, IDM... Mostrar Tudo |
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Palavras-Chave: |
Genetic material (RNA); SsDNA; Viral nucleocapsid phosphoprotein). |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03303naa a2200397 a 4500
001 2135418
005 2022-06-09
008 2021 bl uuuu u00u1 u #d
024 7 $a10.1039/d1qm00665g$2DOI
100 1 $aSOARES, J. C.
245 $aDetection of a SARS-CoV-2 sequence with genosensors using data analysis based on information visualization and machine learning techniques.$h[electronic resource]
260 $c2021
300 $a5658-5670
520 $aWe report on genosensors to detect an ssDNA sequence from the SARS-CoV-2 genome, which mimics the GU280 gp10 gene (coding the viral nucleocapsid phosphoprotein), using four distinct principles of detection and treating the data with information visualization and machine learning techniques. Genosensors were fabricated on either gold (Au) interdigitated electrodes for electrical and electrochemical measurements or on Au nanoparticles on a glass slide for optical measurements. They contained a matrix of 11-mercaptoundecanoic acid (11-MUA) self-assembled monolayer (SAM) onto which a layer of capture probe (cpDNA) sequence was immobilized. Detection was performed using electrical and electrochemical impedance spectroscopies and localized surface plasmon resonance (LSPR). The highest sensitivity was reached with impedance spectroscopy, including using a low-cost (US$ 100) homemade impedance analyzer. Complementary ssDNA sequences were detected with a detection limit of 0.5 aM (0.3 copy per mL). This performance may be attributed to the high sensitivity of the electrical impedance technique combined with an appropriate arrangement of the sequences on the electrodes and hybridization between the complementary sequences, as inferred from polarizationmodulated infrared reflection absorption spectroscopy (PM-IRRAS). The selectivity of the genosensor was confirmed by plotting the impedance spectroscopy data with a multidimensional projection technique (interactive document mapping, IDMAP), where a clear separation was observed among the samples of the complementary DNA sequence at various concentrations and from buffer samples containing a non-complementary sequence and other DNA biomarkers. The diagnosis of SARS-CoV-2 mimicking sequences was also achieved with machine learning techniques applied to scanning electron microscope images taken from genosensors exposed to distinct concentrations of the complementary ssDNA sequences. In summary, the genosensors proposed here are promising for detecting SARS-CoV2 genetic material (RNA) in biological fluids in point-of-care settings
653 $aGenetic material (RNA)
653 $aSsDNA
653 $aViral nucleocapsid phosphoprotein)
700 1 $aSOARES, A. C.
700 1 $aRODRIGUES, V. C.
700 1 $aOITICICA, P. R. A.
700 1 $aRAYMUNDO-PEREIRA, P. A.
700 1 $aBOTT-NETO, J. L.
700 1 $aBUSCAGLIA, L. A.
700 1 $aCASTRO, L. D. C. de
700 1 $aRIBAS, L. C.
700 1 $aSCABINI, L.
700 1 $aBRAZACA, L. C.
700 1 $aCORREA, D. S.
700 1 $aMATTOSO, L. H. C.
700 1 $aOLIVEIRA, M. C. F. de
700 1 $aCARVALHO, A. C. P. L. de
700 1 $aCARRILHO, E.
700 1 $aBRUNO, O. M.
700 1 $aMELENDEZ, M. E.
700 1 $aOLIVEIRA JR, O. N.
773 $tMaterials Chemistry Frontiers$gv. 5, 2021.
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Registro original: |
Embrapa Instrumentação (CNPDIA) |
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
08/12/2016 |
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Data da última atualização: |
11/07/2017 |
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Tipo da produção científica: |
Comunicado Técnico/Recomendações Técnicas |
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Autoria: |
LIEBSCH, D.; OLIVEIRA, E. B. de; MOREIRA, J. M. M. A. P.; MIKICH, S. B. |
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Afiliação: |
DIETER LIEBSCH, UNIVERSIDADE FEDERAL DO PARANÁ; EDILSON BATISTA DE OLIVEIRA, CNPF; JOSE MAURO M AVILA PAZ MOREIRA, CNPF; SANDRA BOS MIKICH, CNPF. |
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Título: |
Inventário e aplicativo computacional para avaliação de danos causados por macaco-prego em plantios de pínus. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Colombo: Embrapa Florestas, 2016. |
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Páginas: |
8 p. |
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Série: |
(Embrapa Florestas. Comunicado técnico, 384). |
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Idioma: |
Português |
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Palavras-Chave: |
Macaco-prego; Sapajus nigritus; SisPinus. |
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Thesagro: |
Pinus Elliottii; Pinus Taeda. |
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Thesaurus NAL: |
Pinus. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/151468/1/CT-384-1353.pdf
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Marc: |
LEADER 00710nam a2200229 a 4500
001 2058247
005 2017-07-11
008 2016 bl uuuu u0uu1 u #d
100 1 $aLIEBSCH, D.
245 $aInventário e aplicativo computacional para avaliação de danos causados por macaco-prego em plantios de pínus.$h[electronic resource]
260 $aColombo: Embrapa Florestas$c2016
300 $a8 p.
490 $a(Embrapa Florestas. Comunicado técnico, 384).
650 $aPinus
650 $aPinus Elliottii
650 $aPinus Taeda
653 $aMacaco-prego
653 $aSapajus nigritus
653 $aSisPinus
700 1 $aOLIVEIRA, E. B. de
700 1 $aMOREIRA, J. M. M. A. P.
700 1 $aMIKICH, S. B.
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